Navigation Links
Crammed with charged DNA, pressure rises inside virus

It could be an artist’s depiction of someone’s stomach before and after a rather decadent meal. But it is a 3-D cryoelectron microscope reconstruction of the cross-section of a virus, before and after cramming itself full of its own DNA.

The virus, phi29, has a tiny motor that pumps its DNA into the capsid—outer shell—during the assembly process. The potential energy of the tightly coiled DNA may help phi29 inject its genetic material into the bacterial cells it infects. Now a team led by physicists at the University of California, San Diego has used laser tweezers to measure the forces exerted by the motor as it pushes the DNA into the capsid.

“The virus’ motor has to do mechanical work to overcome two factors that create resistance,” said Douglas Smith, an assistant professor of physics at UCSD who headed the team that published the discovery this week in the early on-line edition of Proceedings of the National Academy of Sciences. “First, the DNA must be forced to bend. Second, the electrostatic repulsion of the DNA’s negatively charged backbone must be overcome. We found that the positively charged ions in the solution are critical to overcoming this repulsion. Without the right combination of positively charged ions, the virus could not force all of its DNA into the capsid.”

The researchers discovered that the forces in the capsid are slightly higher than predicted by theoretical calculations. They say this may be because the packed DNA is less ordered than assumed in the calculations.


'"/>

Source:University of California - San Diego


Page: 1

Related biology news :

1. Gene therapy to lower blood pressure just enough
2. Use of high hydrostatic pressure to inactivate Hepatitis A virus in oysters studied
3. Researchers learn how blood vessel cells cope with their pressure-packed job
4. Genes affecting blood pressure change as children become adults
5. How nice, brown rice: Study shows rice bran lowers blood pressure in rats
6. Blame the brain for high blood pressure
7. Its OK for men with high blood pressure to have a drink or two, new study finds
8. 8 plants from South Africa may hold potential for treating high blood pressure
9. First real-time view of developing neurons reveals surprises, say Stanford researchers
10. Controlling wildlife trade key to preventing health crises, study says
11. Patient-choice C-section rate rises 36%: HealthGrades study
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Crammed with charged DNA pressure rises inside virus

(Date:6/17/2015)... SAN JOSE, Calif. , June 17, 2015 ... leader in human interface solutions, today announced that ... has adopted the Synaptics ® ClearPad ® ... family of display driver integrated circuits (DDICs) for ... Note Pro. By leveraging ClearPad for full in-cell ...
(Date:6/16/2015)... 16, 2015 Fingerprint Cards (FPC) raises ... it will amount to at least 340 MSEK to that ... rapid increase in market growth and orders received the revenue ... that revenue for 2015 will exceed 1,500 MSEK to an ... 2,200 MSEK. Due to receipt of orders of ...
(Date:6/11/2015)... June 11, 2015 Daon, a global ... that its IdentityX Mobile Authentication Platform v4.0 has ... Alliance is an industry consortium launched in 2013 ... simpler, stronger authentication.  In order to receive certification, ... rigorous series of tests that measure compliance and ...
Breaking Biology News(10 mins):Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 2Synaptics Touchscreen and Display Driver IC Solutions Power Xiaomi's Latest Flagship Smartphones 3Fingerprint Cards Raises the Revenue Guidance for Q2 2015 and for the Full Year 2015 2Fingerprint Cards Raises the Revenue Guidance for Q2 2015 and for the Full Year 2015 3Daon's IdentityX Mobile Authentication Platform One of First Products to be Certified by FIDO Alliance 2
... protective delivery vehicle that shuttles friendly bacteria safely through ... boost for the probiotics industry. The new technology ... drugs and even increase calcium absorption, according to research ... at the University of York this week. The ...
... coli strain that hit Germany in May 2011 ... of collaboration across the scientific community should give insight ... the Society for General Microbiology,s Autumn Conference 2011. ... BGI, China. Their sequence was provided in draft form ...
... this math more evident than in vertebrates, which are programmed ... do not. Snakes, of course, have no digits, and birds ... handle on how these developmental changes are orchestrated in the ... Which digits are they: a thumb with index and middle ...
Cached Biology News:New polymer research could boost probiotics industry 2Crowd-sourcing the E. coli O104:H4 outbreak 2
(Date:6/25/2015)... June 25, 2015 ... -Authentifizierungsdienstleistungen, führte am 24. und 25. Juni ... am Main das 2. Eco System Partner ... Thema Sicherheit für Patienten in Europa und ...      (Logo: http://photos.prnewswire.com/prnh/20150610/748687) , Vertreter ...
(Date:6/25/2015)... /PRNewswire/ - Portage Biotech Inc. ("Portage" or "the Company") ... is pleased to announce that it has completed its ... 36.8 million common shares at US$0.14 each. The private ... on June 15, 2015 as per our press release ... closed on June 24, 2015. The Company is debt ...
(Date:6/25/2015)... Nev. , June 25, 2015  PDL BioPharma, Inc. ... P. McLaughlin , the company,s president and chief executive officer, ... in London , England.  The fireside chat-style ... June 30, 2015 at 8:30 a.m. BST / 3:30 a.m. ... webcast of the presentation, go to the Company,s website at ...
(Date:6/25/2015)... ... June 25, 2015 , ... The fact that adult tissue stem cells cannot ... biology research and regenerative medicine. It is a disquieting truth. Even in ... number of stem cells in transplanted therapeutic cell preparations is unknown. The same ...
Breaking Biology Technology:Symposium zur Richtlinie über gefälschte Arzneimittel - mit Aegate in Deutschland 2Symposium zur Richtlinie über gefälschte Arzneimittel - mit Aegate in Deutschland 3Portage Completes Non-brokered Private Placement in Two Tranches for a Total Proceeds of US$ 5,155,080 2Portage Completes Non-brokered Private Placement in Two Tranches for a Total Proceeds of US$ 5,155,080 3PDL BioPharma to Present at the Nasdaq 32nd Investor Program 2Asymmetrex’s Crowdsourcing Campaign For Evaluating A First Biomarker for Counting Adult Tissue Stem Cells Is Gaining Momentum 2Asymmetrex’s Crowdsourcing Campaign For Evaluating A First Biomarker for Counting Adult Tissue Stem Cells Is Gaining Momentum 3Asymmetrex’s Crowdsourcing Campaign For Evaluating A First Biomarker for Counting Adult Tissue Stem Cells Is Gaining Momentum 4
... Dendreon Corporation (Nasdaq: DNDN ) management will host a conference call ... second quarter 2009 financial results. Access to the discussion may be obtained as ... 11:30 AM ET /8:30 AM PT, Date: ... (domestic) or +1-719-325-4873 (international), Webcast: www.dendreon.com (homepage and investor ...
... , , , PITTSBURGH, July 30 ... the three and six months ended June 30, 2009. , , ... Adjusted diluted EPS, which excludes the impact of certain purchase accounting items ... $0.65 for the three and six months ended June 30, 2009, compared to $0.20 ...
... a weakened strain of the malaria parasite that will be ... developed in collaboration with researchers from the US, Japan and ... Malaria kills more than one million people each year and ... of at least 35 million years of healthy, productive human ...
Cached Biology Technology:Dendreon to Hold Conference Call on Tuesday, August 11, 2009 at 11:30 AM ET 2Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 2Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 3Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 4Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 5Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 6Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 7Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 8Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 9Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 10Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 11Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 12Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 13Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 14Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 15Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 16Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 17Mylan Reports Adjusted Diluted EPS of $0.32 for the Quarter Ended June 30, 2009 18First genetically engineered malaria vaccine to enter human trials 2
... 8 micro-bore tubes to 1 outflow ... tips, Rapid (several millisecond) solution change ... prevention , Solid construction 360, 250 ... For mounting on any micromanipulator Available ...
... (BTI-TN-5B1-4) derived from Trichoplusia ni egg ... be capable of expressing significantly higher ... to other insect cells. High Five ... time as adherent cultures- Quick adaptation into ...
... Racks are ideal for storage and transport ... polypropylene racks withstand extreme temperatures, making them ... may also be autoclaved for use in ... side accommodates 0.5ml microcentrifuge tubes, the other ...
... Signet offers the most comprehensive, ... requirements. We have incorporated unprecedented quality ... capabilities. This includes proven detection chemistries ... to the TechMate protocol. It also ...
Biology Products: