Navigation Links
Conserved amino acids play both structural and mechanistic roles in sandwich-like protein

The question of whether amino acids in sandwich-like proteins are there to stabilize the structure or to speed up the protein-folding process is best answered by "all of the above," according to researchers at Rice University in Houston.

This discovery, reported in today's issue of the Proceedings of the National Academy of Sciences, could benefit future research on treatments for diseases related to misfolded proteins, such as Alzheimer's and Huntington's.

The Rice scientists studied azurin ?a copper-containing protein essential to electron transfer. Azurin is part of a group of proteins that fold into a sandwich-like structure consisting of two sheets of amino acids meshed together. Nearly 70 superfamilies of proteins of varying makeup have this sandwich-like structure, but they all have eight particular amino acids in common. Previous studies had shown that these eight amino acids were important to define the sandwich-like structure, but the exact role was unknown.

"Why are these eight amino acids invariant across all sandwich-like proteins?" asked principal investigator Pernilla Wittung-Stafshede, associate professor of biochemistry and cell biology. "Are they conserved to direct the protein-folding reaction, or are they selected to stabilize the final protein structure? In our paper, we unravel an unprecedented answer to this question."

Wittung-Stafshede and graduate student Corey Wilson analyzed the purpose of six of the eight amino acids by exchanging a nonessential amino acid for each of them and monitoring the effect on the protein structure. (For technical reasons, the other two amino acids could not be studied.) The researchers found that three of the amino acids are important for stabilizing the final structure of the protein, and three serve to direct the process of protein folding.

"We directly demonstrated that in one protein within the large sandwich-like protein family, evolution has indeed preserved amino acid
'"/>

Source:Rice University


Page: 1 2

Related biology news :

1. New technique helps researchers determine amino-acid charge
2. Use of amino acid supplement following a heart attack provides no benefit, may be harmful
3. Changes in amino acids in the 1918 influenza virus cut transmission
4. Amino acids in nectar enhance butterfly fecundity: A long awaited link
5. Researchers create pigs that produce heart-healthy omega-3 fatty acids
6. A new window into structural plasticity in the adult visual cortex
7. Meditation associated with structural changes in brain
8. New technology used to construct the first map of structural variation in the human genome
9. Researchers image molecular motor structural changes
10. Scripps research study reveals structural dynamics of single prion molecules
11. Flies on speed offer insight into the roles of dopamine in sleep and arousal
Post Your Comments:
(Date:1/22/2015)... Wis. , Jan. 15, 2015  BellBrook Labs, ... drug discovery, announced the launch of a TR-FRET ... the company,s Transcreener UDP Assay, a high throughput ... The new assay will allow for sensitive detection ...
(Date:1/22/2015)... , January 22, 2015 , ... year  The European Patent Office to present a video ... British nominations to be featured: Christofer Toumazou and ... former finalists and winners of the Award   Starting ...
(Date:12/22/2014)... Inc. (NASDAQ: NXTD and NXTDW) ("NXT-ID" or ... mobile commerce market, announces it has retained famous pickpocket, entertainer ... International CES debut of the Wocket™ biometric smart wallet.  ... NXT-ID booth January 6th and 7 th , 2015, demonstrating ...
Breaking Biology News(10 mins):BellBrook Labs Launches a High Throughput Screening Assay for Glycosyltransferases with TR-FRET Readout 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4
... in The FASEB Journal ( https://www.fasebj.org ) ... genetic disease that causes increased susceptibility to blue light, ... or Goldman-Favre Syndrome. In the report, scientists found that ... of rods and cones in the retina was reduced ...
... Annual Meeting & OTO EXPO of the American Academy ... the largest meeting of ear, nose, and throat doctors ... San Francisco, CA. Featuring more than 386 scientific ... hours for attendees, the annual meeting is a unique ...
... group of institutions led by the Marine Science Institute (MSI) at ... nearly $7 million grant to better understand how oil spills disperse ... ecology of the Gulf. The grant, which is the largest ... Gulf of Mexico Research Initiative (GRI) established by BP in the ...
Cached Biology News:Scientists unravel the cause of rare genetic disease: Goldman-Favre Syndrome explained 22011 AAO-HNSF miniseminars 22011 AAO-HNSF miniseminars 32011 AAO-HNSF miniseminars 42011 AAO-HNSF miniseminars 52011 AAO-HNSF miniseminars 62011 AAO-HNSF miniseminars 7Marine Science Institute receives $7 Million grant to study the impact of the Deepwater Horizon 2
(Date:1/22/2015)... NEW BRUNSWICK , Nueva Jersey , 22 ... Award for Biomedical Research abre hoy su llamada ... las personas cuya investigación científica ha hecho, o tiene ... de la salud humana. Las nominaciones se aceptarán hasta ...
(Date:1/22/2015)... , Jan. 22, 2015   GenoSpace , a precision ... tools to enable the broad use of genomic, imaging and ... the appointment of Michelle Munson , CEO of Aspera, ... Logo - http://photos.prnewswire.com/prnh/20150122/170713 ...
(Date:12/24/2014)... , Dec. 24, 2014  United Therapeutics Corporation (NASDAQ: ... MDT ) has submitted a pre-market approval ... for the use of Medtronic,s SynchroMed ® II ... for use with United Therapeutics, Remodulin ® (treprostinil) ...
(Date:12/24/2014)... Wash. , Dec. 24, 2014   BioLife Solutions , ... and marketer of proprietary clinical grade hypothermic storage ... shipping products for cells and tissues  ("BioLife" or the ... Meeting of Stockholders on May 4, 2015 (the "Annual Meeting"). ...
Breaking Biology Technology:El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4GenoSpace Expands Board with Appointment of Michelle Munson 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 3United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 4BioLife Solutions Sets Date for Annual Meeting of Stockholders 2
... WASHINGTON and NEW YORK, Feb. 6, 2012 ... Diagnostics are collaborating with leading academic medical ... and cerebrospinal fluid-based molecular diagnostics technology in ... explore the capabilities of Exosome RNA biofluid-based ...
... 2012 UV Flu Technologies, Inc. ( OTCBB: ... ahead of schedule in its continuing program to eliminate all debt. ... the remaining and final convertible note, in the amount of $32,500 ... we have made in the last twelve months is nothing short ...
... LOUIS, Feb. 6, 2012 RiverVest Venture Partners ... for more than $1 billion, generated upfront returns of up ... "RiverVest has really separated itself from the pack," ... Healthcare & Life Sciences Fund, which is an investor in ...
Cached Biology Technology:Accelerate Brain Cancer Cure and Exosome Diagnostics Collaborate to Advance Clinical Studies of Exosome Biofluid Molecular Diagnostics Technology in Brain Cancer 2Accelerate Brain Cancer Cure and Exosome Diagnostics Collaborate to Advance Clinical Studies of Exosome Biofluid Molecular Diagnostics Technology in Brain Cancer 3Accelerate Brain Cancer Cure and Exosome Diagnostics Collaborate to Advance Clinical Studies of Exosome Biofluid Molecular Diagnostics Technology in Brain Cancer 4UV Flu Technologies Continues Aggressive Program to Pay off Convertible Debt 2UV Flu Technologies Continues Aggressive Program to Pay off Convertible Debt 3RiverVest Venture Partners Sells Four Successful Life Science Companies for More than $1 Billion 2RiverVest Venture Partners Sells Four Successful Life Science Companies for More than $1 Billion 3
MAb to Vasopressin Preservative: NaN3...
... Anti-AP-2 α, clone 8G8/5 GenBank ... Bacterially made human AP-2alpha/gamma fusion protein corresponding ... residues 278-450 of human AP-2γ Formulation: 0.1M ... azide before the addition of glycerol to ...
Mouse monoclonal [1C6/2] to Rad17 ( Abpromise for all tested applications). entrezGeneID: 5884 SwissProtID: Q9UPF5...
MMP-2 / MMP-9 Inhibitor IV is a potent, selective, slow binding and mechanism based inhibitor of human gelatinases. • Molecular weight: 306.4 • Molecular Formula: C(15)H(14)O(3)S(2). &bul...
Biology Products: