Navigation Links
Color-blind method opens new doors in DNA sequencing

nce.

Most sequencing today is done with one laser and optics to separate the dyes into the four colors, blue, green, yellow, and red. A common problem with the technique is that the color of light emitted by the dyes is similar. Even with complex computer programs to assist in deciphering the signals, this "cross-talk" between the dyes results in subtle variations that can cause nucleotides to be miscalled.

The new method developed at BCM and Rice, called pulsed multiline excitation, uses four lasers, each matched to a particular dye. PME enables the researchers to take advantage of the entire visible spectrum, eliminating the problem of cross-talk between dyes, said Metzker.

Because there are four lasers, scientists can manipulate the system so that each dye gives the same intensity of fluorescent signal, eliminating the need for further software processing to yield readable sequence information.

"Genome sequencing, by its very nature, is a process that begs for precision, and the number of mistakes that can be tolerated is extremely low," said Curl, University Professor, the Kenneth S. Pitzer-Schlumberger Professor of Natural Sciences and professor of chemistry. "Our new method does away with identification problems altogether, because the imaging is very clean."

Metzker said, "We have built a highly sensitive instrument for the measuring of fluorescence, because PME gives brighter signals and collects more of that signal by eliminating the need for a prism to separate the light into colors."

Metzker is also seeking to develop a chip-based imager than could be used in his overall project on sequencing-by-synthesis (SBS), which is funded by the National Human Genome Research Institute. SBS could lead to the ability to sequence an individual's own genome rapidly and inexpensively.

Metzker and the major developers of this technology filed a patent on PME in 2001, which has been exclusively licensed to LaserGen f
'"/>

Source:Baylor College of Medicine


Page: 1 2 3

Related biology news :

1. New imaging method gives early indication if brain cancer therapy is effective, U-M study shows
2. Studies reveal methods viruses use to sidestep immune system
3. An entropy-based gene selection method for cancer classification using microarray data
4. New methods of gene delivery using lasers
5. Breakthrough method in nanoparticle synthesis paves the way for new pharmaceutical and biomedical applications
6. Shift of weather patterns necessitates rethinking of reforestation methods
7. Scientists use manufacturing methods to reconstruct mastodon
8. Researchers develop promising new gene network analysis method
9. New method shows it is possible to grow bone for grafts within a patients body
10. Researchers develop new method for facile identification of proteins in bacterial cells
11. A novel method to measure circadian cycles
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Color blind method opens new doors DNA sequencing

(Date:5/21/2015)... 2015 The Sync Project™ , a ... health, today announced a collaborative partnership with Berklee,s ... center on collaboration on original research, joint course development ... the collaboration, The Sync Project and BerkleeICE are exploring ... in the 2015-2016 academic year.  The ...
(Date:5/19/2015)... , May 19, 2015 ... announced the addition of the  "Genetic Testing ... ,     (Logo: http://photos.prnewswire.com/prnh/20130307/600769) , ,A recent report, ... in-depth analysis of the current and future ... gene-based tests, their working principles and types ...
(Date:5/11/2015)... 11, 2015  Through a well-rounded UAS delegation representing private ... a strong showing at AUVSI,s Unmanned 2015 conference last week ... Ohio,s UAS industry met with over 200 ... points along the UAS ecosystem. "Our message ... President for Aerospace Rich Knoll . "If you want ...
Breaking Biology News(10 mins):The Sync Project Announces Partnership with Berklee College of Music's Institute for Creative Entrepreneurship 2The Sync Project Announces Partnership with Berklee College of Music's Institute for Creative Entrepreneurship 3Global Genetic Testing Market Outlook 2018 2Ohio Flies High at Unmanned '15, Sets Stage for Ohio UAS Conference 2
... This three-day international symposium is designed to bring ... examine how new advances in plant science research and ... African agriculture. The symposium will present recent advances in ... for the development of agriculture in Africa, as well ...
... associated with the discovery of the oldest known, nearly complete ... took its first steps toward bipedalism not on the open, ... Charles Darwin hypothesized, but in a wooded landscape. ... said University of Illinois anthropology professor Stanley Ambrose, a co-author ...
... weekend warriors: the simple act of exercise and not ... a new University of Florida study finds. People ... gaining strength or boosting cardiovascular fitness feel just as ... said Heather Hausenblas, a UF exercise psychologist. Her study ...
Cached Biology News:Agriculture: Africa's 'engine for growth' 2Early hominid first walked on 2 legs in the woods 2Early hominid first walked on 2 legs in the woods 3UF study: Exercise improves body image for fit and unfit alike 2UF study: Exercise improves body image for fit and unfit alike 3
(Date:5/29/2015)... 29, 2015 Results from a Phase ... and its ability to reduce the size of melanoma ... published in the Journal of Clinical Oncology. , ... IIIB, IIIC and IV melanoma, a potentially fatal form ... was to evaluate the safety and efficacy of the ...
(Date:5/28/2015)... DUBLIN , May 28, 2015 /PRNewswire/ ... http://www.researchandmarkets.com/research/7k46bt/therapeutic_drug ) has announced the addition of ... Monitoring - Technologies, Markets, and Companies" ... http://photos.prnewswire.com/prnh/20130307/600769) , This report deals ... specialty, aimed at improving patient care by ...
(Date:5/28/2015)... DUBLIN , May 27, 2015 Research ... the "Animal Genetics Market by Products, by Testing ... their offering. The global animal genetics market ... 2014 and grow at a CAGR of 8-9% during ... This market is mainly driven by increasing animal protein ...
(Date:5/28/2015)... , May 28, 2015 Celsion Corporation ... it has received commitments from two institutional healthcare investors ... the Company,s common stock in an at-the-market registered direct ... purchase common stock. The Company entered into ... which the Company agreed to sell an aggregate of ...
Breaking Biology Technology:Study Published in Journal of Clinical Oncology Reports Talimogene Laherparepvec Improvesdurable Responses in Patients with Advanced Melanoma 2Global Therapeutic Drug Monitoring Market 2015-2024 2Global Animal Genetics (Canine, Equine, Poultry, Porcine, Bovine, and Genetic Material) Market 2015 - Global Forecast to 2020 2Celsion Corporation Announces $8 Million At-The-Market Registered Direct Offering 2Celsion Corporation Announces $8 Million At-The-Market Registered Direct Offering 3
... 23, 2010Scientists at the Gladstone Institute of Neurological Disease ... in Alzheimer,s disease (AD) and other neurodegenerative diseases. The ... "We examined a protein called tau that ... Gan, PhD, senior author on the study. "Tau forms ...
... LONDON and SAN FRANCISCO, September 22, 2010 ... ... ... Results are keyed by longUrl, so we need to grab the first one. for (var r in ...
... SINGAPORE, Sept. 23 S*BIO Pte Ltd today ... that will support the continued development of S*BIO,s ... commitment reflects the international investment community,s strong interest ... through clinical development and the quality of our ...
Cached Biology Technology:Gladstone scientists identify strategy to reduce toxic proteins associated with Alzheimer's disease 2Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 2Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 3Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 4Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 5Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 6Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 7Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 8Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 9Data Published Today Reveal That Novel Oral Therapy Fostamatinib Demonstrates Positive Response in Rheumatoid Arthritis Patients 10S*BIO Announces US$5 Million Investment by Mitsui Ventures 2S*BIO Announces US$5 Million Investment by Mitsui Ventures 3
... S1 Nuclease degrades single-stranded DNA ... products. Double-stranded nucleic acids (DNA:DNA, DNA:RNA ... except with extremely high concentrations of ... to remove single-stranded termini from double-stranded ...
SP6 RNA Polymerase, 50 l, 20 U/l ,Cap Scribe Buffer, 100 l, 5x conc....
Miniature FRENCH Pressure Cell with 3.7ml capacity; pressure up to 20,000 psi....
...
Biology Products: