Navigation Links
Cloned mice created from fully differentiated cells, a milestone in cloning research

rchers compared the efficiency for cloning mice using a fully differentiated blood cell called a granulocyte with its ancestor cells at different stages: hematopoietic stem cells, which are found in bone marrow and give rise to all red and white blood cells, and progenitor cells. Granulocytes are well characterized white blood cells unique for their segmented nuclei and the numerous granules in the cells' cytoplasm.

Surprisingly, the granulocytes were the most efficient donor cells for nuclear transfer among the different lineage cells, with 35 to 39 percent becoming a blastocyst, an early embryo consisting of about 100 to 150 cells, compared to 11 percent for the progenitor cells and only 4 percent for the stem cells. Only the granulocytes were able to produce two live cloned pups, although both died within a few hours of birth. As a control, the researchers performed nuclear transfer using embryonic stem cells; 49 percent developed to the blastocyst stage and 18 cloned pups were born.

"Our results clearly demonstrate that there is no apparent advantage in using either adult stem cells or progenitor cells over fully differentiated cells as nuclear donors. To the contrary, we found that cloned pups can be produced from adult, fully differentiated somatic cells, a conclusion that goes against popular opinion and current hypotheses," says Dr. Yang, animal science professor, director of the University of Connecticut's Center for Regenerative Biology and co-corresponding author of the study.

"Even we were surprised to find fully differentiated cells were more efficient for cloning, because granulocytes are not capable of dividing. In fact, we repeated our experiments six times just to be sure. Now we can say with near certainty that a fully differentiated cell such as a granulocyte retains the genetic capacity for becoming like a seed that can give rise to all cell types necessary for the development of an entire organism," adds co-correspondin
'"/>

Source:University of Pittsburgh Medical Center


Page: 1 2 3 4

Related biology news :

1. Cloned stem cells prove identical to fertilized stem cells
2. Molecular research suggest shift needed in how some drugs are created
3. All placebos not created alike
4. Pinball protons created by ultraviolet rays and other causes can lead to DNA damage
5. Polynesia explorers created worldwide web of scientific knowledge
6. Sperm created in the laboratory from embryonic stem cells produce viable progeny
7. Researcher examines polymers created with poultry feathers
8. Early-stage sperm cells created from human bone marrow
9. New, automated tool successfully classifies and relates proteins in unprecedented way
10. Biomarkers isolated from saliva successfully predict oral and breast cancer
11. UN successfully tests green pesticide against locusts
Post Your Comments:
(Date:5/21/2015)... 2015 The Sync Project™ , a ... health, today announced a collaborative partnership with Berklee,s ... center on collaboration on original research, joint course development ... the collaboration, The Sync Project and BerkleeICE are exploring ... in the 2015-2016 academic year.  The ...
(Date:5/19/2015)... , May 19, 2015 ... announced the addition of the  "Genetic Testing ... ,     (Logo: http://photos.prnewswire.com/prnh/20130307/600769) , ,A recent report, ... in-depth analysis of the current and future ... gene-based tests, their working principles and types ...
(Date:5/11/2015)... , May 11, 2015  Through a well-rounded UAS delegation ... had a strong showing at AUVSI,s Unmanned 2015 conference ... of Ohio,s UAS industry met with ... from all points along the UAS ecosystem. ... (DDC) Vice President for Aerospace Rich Knoll . "If ...
Breaking Biology News(10 mins):The Sync Project Announces Partnership with Berklee College of Music's Institute for Creative Entrepreneurship 2The Sync Project Announces Partnership with Berklee College of Music's Institute for Creative Entrepreneurship 3Global Genetic Testing Market Outlook 2018 2Ohio Flies High at Unmanned '15, Sets Stage for Ohio UAS Conference 2
... called ,pestilences, three are historically recognized as due to ... 19th to 21st centuries AD there were microbiological evidences ... pestis . "For a long time scholars from different ... of the past pandemics. Only ancient DNA analyses carried ...
... , May 9, 2013 Wexford Science & ... in the Wake Forest Innovation Quarter , today ... buildings currently underway represents an additional $150 million investment ... Wexford,s total investment in the Innovation Quarter to more ...
... NY and San Diego, CA A large, multi-institutional research ... a sweeping analysis in the current issue of the journal ... off to direct early human development. Led by Bing Ren ... The Salk Institute for Biological Studies and James Thomson of ...
Cached Biology News:Scientists confirm that the Justinianic Plague was caused by the bacterium Yersinia pestis 2Wexford Science & Technology Makes $150 Million Investment in Wake Forest Innovation Quarter 2Wexford Science & Technology Makes $150 Million Investment in Wake Forest Innovation Quarter 3Mapping the embryonic epigenome 2Mapping the embryonic epigenome 3
(Date:5/26/2015)... May 26, 2015 Innovacyn, a ... in the human and animal health industries, announced ... Future Innovators of Agriculture College Scholarship Award. The ... who exhibits a passionate commitment to the agriculture ... This year’s winners, Alexis Pedrow from Anderson County ...
(Date:5/26/2015)... 2015  Sangamo BioSciences, Inc. (NASDAQ: SGMO ) announced ... will provide an update on the progress of Sangamo,s ZFP ... company,s business strategy at 1:30pm ET on Monday, June 1, ... is being held in New York, NY ... may be accessed via a link on the Sangamo BioSciences ...
(Date:5/26/2015)... China , May 26, 2015 Tianyin ... company that specializes in the patented biopharmaceutical, modernized traditional ... (API), announced that the Company,s Annual Meeting of Shareholders ... scheduled to be held at the Company,s office located ... conference call, at 9:00 AM (local time in ...
(Date:5/26/2015)... , May 26, 2015   PDI ... solutions, today announced that Oakwood Healthcare in ... as the winning entry in Delivering ... professionals who display excellence in building collaborative relationships ... teams in healthcare facilities.  Oakwood ...
Breaking Biology Technology:Innovacyn Announces Winners Of This Year’s Vetericyn® College Scholarship Award 2Innovacyn Announces Winners Of This Year’s Vetericyn® College Scholarship Award 3Sangamo BioSciences Announces Presentation At The Jefferies 2015 Global Healthcare Conference 2TPI Adjourned Its 2014 Annual Shareholder Meeting and Received Notice of Non-Compliance with NYSE MKT Continued Listing Requirements 2TPI Adjourned Its 2014 Annual Shareholder Meeting and Received Notice of Non-Compliance with NYSE MKT Continued Listing Requirements 3PDI Announces Winner in Delivering the Difference Contest 2PDI Announces Winner in Delivering the Difference Contest 3
... Dec. 20 CuraGen Corporation,(Nasdaq: CRGN ), ... the appointment of Sean Cassidy, as Vice,President and ... announced today that David M. Wurzer will step ... pursue other opportunities, but will remain,with the Company ...
... Human Genome,Sciences, Inc. (Nasdaq: HGSI ) ... in a Phase 2 clinical trial of HGS-ETR1 ... carboplatin as,first-line therapy in patients with advanced non-small ... who are newly diagnosed with non-small cell,lung cancer ...
... Northwest,Biotherapeutics, Inc. (OTC Bulletin Board: NWBO; ... today that Lonza Group AG has,withdrawn all ... and other NWBT DCVax(R) products other than ... vaccine products infringed,certain gene expression technology patents ...
Cached Biology Technology:CuraGen Corporation Announces Appointment of Sean Cassidy as CFO 2CuraGen Corporation Announces Appointment of Sean Cassidy as CFO 3CuraGen Corporation Announces Appointment of Sean Cassidy as CFO 4Human Genome Sciences Initiates Second Randomized Phase 2 Trial of HGS-ETR1 in Combination With Chemotherapy 2Human Genome Sciences Initiates Second Randomized Phase 2 Trial of HGS-ETR1 in Combination With Chemotherapy 3Human Genome Sciences Initiates Second Randomized Phase 2 Trial of HGS-ETR1 in Combination With Chemotherapy 4Human Genome Sciences Initiates Second Randomized Phase 2 Trial of HGS-ETR1 in Combination With Chemotherapy 5Human Genome Sciences Initiates Second Randomized Phase 2 Trial of HGS-ETR1 in Combination With Chemotherapy 6Lonza Withdraws Majority of Claims in Patent Lawsuit Against Northwest Biotherapeutics 2Lonza Withdraws Majority of Claims in Patent Lawsuit Against Northwest Biotherapeutics 3Lonza Withdraws Majority of Claims in Patent Lawsuit Against Northwest Biotherapeutics 4
Request Info...
... Provides a buffered anti-freeze environment for enzyme ... a liquid, low viscosity solution at -20C ... necessary and sampling is convenient , ... long-term storage Protect enzyme conjugates during ...
Request Info...
... system, which is based on a new flow-through ... performing in situ hybridization of tissues on microscope ... the study of gene expression, in situ hybridization ... and can be used to validate and confirm ...
Biology Products: