Navigation Links
Biofuel cells without the bio cells

Proteins keep cells humming. Some are enzymes that taxi electrons to chemicals outside the cell, to discharge excess energy generated during metabolism. This maintains energy flow in the cell and, in turn, keeps the cell alive.

The process has worked a little like that slogan for a hyper-electrified desert gambling town: What happens in the cellular environs stays there.

Now, scientists for the first time have observed this electricity-shuttling process taking place sans cells, in purified proteins removed from the outer membrane of the versatile, metal-altering soil bacterium Shewanella oneidensis. Reporting in the current advance online edition of the Journal of the American Chemical Society, they suggest that proteins rendered portable from the organisms that spawned them could make miniature bioreactor cells feasible.

"We show that you can directly transfer electrons to a mineral using a purified protein, and I don't think anyone had shown that before," said Thomas Squier, senior author and lab fellow at the Department of Energy's Pacific Northwest National Laboratory.

The feat is the bacterial equivalent of removing lungs and coaxing the disembodied tissue to breathe.

Squier and principal authors Yijia Xiong and Liang Shi, PNNL staff scientists, discovered that the proteins, outer membrane c-type cytochrome A, or OmcA, formed a dense coating on the iron-rich mineral hematite. The metal in the mineral acts as an "acceptor," or dumping point, for thousands of trillions of electrons per square centimeter shuttled by the OmcA-donor. The function is a relic of respiration, in which the cell depends on the protein to dump electrons to maintain a steady flow of energy and prevent the organism-damaging accumulation of electrons.

PNNL staff scientist and co-author Uljana Mayer devised new tagging methods that enabled the team to isolate sufficient amounts of protein. The tags also allowed fast measurements of protein- mineral binding.

The researchers supplied the protein with energy--directly as electrons or in the form of a natural cellular fuel called NADH--and only during binding detected charge-transfer from protein to mineral, through a combination of techniques that included FCS, or fluorescent correlation spectroscopy, and confocal microscopy. These yielded a "fluorescence intensity trace" whose brightness depended entirely on whether hematite was available to bind with OmcA in solution. No hematite, dim; hematite, bright.

How bright?

"The peak current, or flux, doesn't run long, just a few seconds," Squier said, "but flux is at least as good as what you would find in the most efficient bioreactors, which rely on living bacteria."

Biological fuel cells, or biofuel cells, are not yet powerful enough to be commercially viable but they offer the promise of breaking down sewage and other biological waste while generating electricity directly from the same process. An example, Squier said, is a self-powering sewage treatment plant.

Using pure protein opens up the possibility of shrinking biofuel cells to power small electronic devices, Squier said. Whole-organism biofuel cells require engineers to design a space-adding membrane that prevents unwanted reactions between fuel, the charge-transporting agent and the electron-accepting metal, the latter being the electrode that carries the electricity to the device. In purified protein fuel cells, the seal made by the protein coating on the electrode effectively acts in place of the membrane.


'"/>

Source:DOE/Pacific Northwest National Laboratory


Related biology news :

1. Biofuels can replace about 30 percent of fuel needs with significant research and policy effort
2. Biofuels researcher searches for new sources
3. Wisconsin scientists grow critical nerve cells
4. Spleen may be source of versatile stem cells
5. Researchers discover way to make cells in the eye sensitive to light
6. Priming embryonic stem cells to fulfill their promise
7. Lack of enzyme turns fat cells into fat burners
8. Poor prenatal nutrition permanently damages function of insulin-producing cells in the pancreas
9. Elusive HIV shape change revealed; Key clue to how virus infects cells
10. Mouse brain cells rapidly recover after Alzheimers plaques are cleared
11. Enzyme allows B cells to resist death, leading to leukemia
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:11/29/2016)... , November 29, 2016 Nearly one billion matches ... Reading ... DERMALOG ... of an efficient Identity Management. (PRNewsFoto/DERMALOG Identification Systems) ... DERMALOG is Germany's largest Multi-Biometric supplier: The company's ...
(Date:11/22/2016)... According to the new market research report "Biometric System Market ... Multi-Factor), Component (Hardware and Software), Function (Contact and Non-contact), Application, and Region ... to grow from USD 10.74 Billion in 2015 to reach USD 32.73 ... Continue Reading ... ...
(Date:11/19/2016)... -- Securus Technologies, a leading provider of civil and ... and monitoring, announced today that it has offered a ... independent technology judge determine who has the largest and ... calling platform, and the best customer service. ... what we do – which clearly is not the ...
Breaking Biology News(10 mins):
(Date:12/8/2016)... ... December 08, 2016 , ... Microbial genomics ... Awards. uBiome is one of just six company finalists in the Health & ... to uBiome, companies nominated as finalists in this year’s awards include Google, SpaceX, ...
(Date:12/8/2016)... -- Soligenix, Inc. (OTCQB: SNGX) (Soligenix or the Company), ... products to treat rare diseases where there is an ... hosting an Investor Webcast Event Friday, December 16, 2016, ... defense regulators (IDRs) as a new drug class, as ... recently announced and published Phase 2 clinical data for ...
(Date:12/8/2016)... Eutilex Co. Ltd. today announced that it ... A financing. This financing round included participation from DS ... Bio Angel. This new funding brings the total capital ... since its founding in 2015. The ... commercialization of its immuno-oncology programs, expand its R&D capabilities ...
(Date:12/7/2016)... ... 07, 2016 , ... ACEA Biosciences, Inc. presented today updated ... trial for its lead drug candidate, AC0010, at the World Conference on Lung ... determine the safety, antitumor activity, and recommended phase II dosage of AC0010 in ...
Breaking Biology Technology: