Navigation Links
Bioelectronic ‘signatures?hold key to DNA mutations

A new method to identify DNA mutations may shepherd in an era of small, portable, electronic devices for the rapid screening and identification of genes that harbor disease.

Joseph Wang, director of the Center for Bioelectronics and Biosensors at the Biodesign Institute at ASU, led a team effort that successfully merged work in the fields of biosensors, electronics, and nanotechnology to fashion nanocrystals that can act as “DNA biosensors?by electronically recognizing subtle mutations in the DNA. This creates enormous potential for applications such as the diagnosis and treatment of genetic diseases, detection of infectious agents and reliable forensic analysis.

Wang, who recently was recruited to the Biodesign Institute and serves a joint appointment as professor in the chemical and materials department at the Ira A. Fulton School of Engineering and the Department of Chemistry, is a renowned expert in nanomaterial-based biosensors that operate at the scale of a thousand times smaller than the width of a human hair. He wrote 660 papers and has 12 patents to his credit, including involvement in the development of the first noninvasive biosensor for diabetes, the FDA approved Gluco Watch, which monitors glucose levels through human sweat.

“The ultimate goal is to make something similar to a hand-held glucose monitor for future genetic testing,?Wang says. “The electronic detection of DNA is a thing of beauty. You can make it small, low-power, inexpensive and robust.?/p>

Among the keys to unearthing the mysteries behind individual genetic variation and diseases like cancer are fine differences ?single nucleotide polymorphisms, or “SNPs??buried within the 3 billion chemical bases of DNA comprising the human genome. Not every SNP found will necessarily cause a mutation or determine our eye or hair color ?but, on average, SNPs occur about once in every 1,000 DNA bases, adding up to 3 million potential individual differences across the human geno me. Wang’s method allows for an accurate, ultra-sensitive, rapid and low-cost identification of these SNP variants.

“The novelty of the approach is the combination of the nanocrystal tagging of DNA to create electro-diverse signatures and combining them with a fast, portable, low-cost electronic detection,?Wang says.

To achieve the desired results, Wang and his researchers custom-made several individual nanocrystals, known as quantum dots, from four heavy metal salts of lead, cadmium, zinc and copper. Such nanocrystals were selected owing to their ability to yield distinct electronic signatures, with four well-resolved current peaks. Next, the nanocrystals are piggybacked onto individual DNA bases; these DNA bases ?each carrying a single nanocrystal ?bind to a DNA sample and cause minute electrical current changes in the nanocrystal that can be measured with an electrode. The individual base-conjugated nanocrystals are added sequentially to any DNA sample of interest, generating an electronic “fingerprint?that rapidly identifies all possible combinations of SNPs.

Wang’s results recently were featured as the cover story in the March issue of Analytical Chemistry and were published earlier this winter in the Journal of the American Chemical Society. The article can be found on the Web at (http://pubs3.acs.org/acs/journals/doilookup?in_doi=10.1021/ja043780a).


'"/>

Source:Arizona State University


Related biology news :

1. Novel technology detects human DNA mutations
2. Multi-species genome comparison sheds new light on evolutionary processes, cancer mutations
3. New view of cancer: Epigenetic changes come before mutations
4. ASU researchers wire DNA to identify mutations
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/3/2016)... May 3, 2016  Neurotechnology, a provider of ... MegaMatcher Automated Biometric Identification System (ABIS) , a ... projects. MegaMatcher ABIS can process multiple complex biometric ... combination of fingerprint, face or iris biometrics. It ... and MegaMatcher Accelerator , which have ...
(Date:4/26/2016)... 2016 Research and Markets has ... Market 2016-2020"  report to their offering.  , ,     ... The analysts forecast the global multimodal biometrics market ... the period 2016-2020.  Multimodal biometrics is ... as the healthcare, BFSI, transportation, automotive, and government ...
(Date:4/14/2016)... Israel , April 14, 2016 ... Authentication and Malware Detection, today announced the appointment of ... assumed the new role. Goldwerger,s leadership appointment ... on the heels of the deployment of its platform ... BioCatch,s behavioral biometric technology, which discerns unique cognitive and ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... Apellis Pharmaceuticals, Inc. today announced positive ... its complement C3 inhibitor, APL-2. The trials were ... studies designed to assess the safety, tolerability, pharmacokinetics ... healthy adult volunteers. Forty subjects were ... dose (ranging from 45 to 1,440mg) or repeated ...
(Date:6/23/2016)... 23, 2016 On Wednesday, June 22, ... down 0.22%; the Dow Jones Industrial Average edged 0.27% lower ... 2,085.45, down 0.17%. Stock-Callers.com has initiated coverage on the following ... Therapeutics (NASDAQ: NKTR ), Aralez Pharmaceuticals Inc. (NASDAQ: ... BIND ). Learn more about these stocks by accessing ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... a new line of intelligent tools designed, tuned and optimized exclusively for Okuma ... 12–17 in Chicago. The result of a collaboration among several companies with expertise ...
(Date:6/22/2016)... DIEGO , June 22, 2016 ... that will allow them to produce up to ... from one lot within one week. These high-quality, ... time laboriously preparing cells and spend more time ... possible through a proprietary, high-volume manufacturing process that ...
Breaking Biology Technology: