Navigation Links
Bioelectronic ‘signatures?hold key to DNA mutations

A new method to identify DNA mutations may shepherd in an era of small, portable, electronic devices for the rapid screening and identification of genes that harbor disease.

Joseph Wang, director of the Center for Bioelectronics and Biosensors at the Biodesign Institute at ASU, led a team effort that successfully merged work in the fields of biosensors, electronics, and nanotechnology to fashion nanocrystals that can act as “DNA biosensors?by electronically recognizing subtle mutations in the DNA. This creates enormous potential for applications such as the diagnosis and treatment of genetic diseases, detection of infectious agents and reliable forensic analysis.

Wang, who recently was recruited to the Biodesign Institute and serves a joint appointment as professor in the chemical and materials department at the Ira A. Fulton School of Engineering and the Department of Chemistry, is a renowned expert in nanomaterial-based biosensors that operate at the scale of a thousand times smaller than the width of a human hair. He wrote 660 papers and has 12 patents to his credit, including involvement in the development of the first noninvasive biosensor for diabetes, the FDA approved Gluco Watch, which monitors glucose levels through human sweat.

“The ultimate goal is to make something similar to a hand-held glucose monitor for future genetic testing,?Wang says. “The electronic detection of DNA is a thing of beauty. You can make it small, low-power, inexpensive and robust.?/p>

Among the keys to unearthing the mysteries behind individual genetic variation and diseases like cancer are fine differences ?single nucleotide polymorphisms, or “SNPs??buried within the 3 billion chemical bases of DNA comprising the human genome. Not every SNP found will necessarily cause a mutation or determine our eye or hair color ?but, on average, SNPs occur about once in every 1,000 DNA bases, adding up to 3 million potential individual differences across the human geno me. Wang’s method allows for an accurate, ultra-sensitive, rapid and low-cost identification of these SNP variants.

“The novelty of the approach is the combination of the nanocrystal tagging of DNA to create electro-diverse signatures and combining them with a fast, portable, low-cost electronic detection,?Wang says.

To achieve the desired results, Wang and his researchers custom-made several individual nanocrystals, known as quantum dots, from four heavy metal salts of lead, cadmium, zinc and copper. Such nanocrystals were selected owing to their ability to yield distinct electronic signatures, with four well-resolved current peaks. Next, the nanocrystals are piggybacked onto individual DNA bases; these DNA bases ?each carrying a single nanocrystal ?bind to a DNA sample and cause minute electrical current changes in the nanocrystal that can be measured with an electrode. The individual base-conjugated nanocrystals are added sequentially to any DNA sample of interest, generating an electronic “fingerprint?that rapidly identifies all possible combinations of SNPs.

Wang’s results recently were featured as the cover story in the March issue of Analytical Chemistry and were published earlier this winter in the Journal of the American Chemical Society. The article can be found on the Web at (http://pubs3.acs.org/acs/journals/doilookup?in_doi=10.1021/ja043780a).


'"/>

Source:Arizona State University


Related biology news :

1. Novel technology detects human DNA mutations
2. Multi-species genome comparison sheds new light on evolutionary processes, cancer mutations
3. New view of cancer: Epigenetic changes come before mutations
4. ASU researchers wire DNA to identify mutations
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/4/2017)... Solutions, a global clinical research organization (CRO), announces the launch of ... 4, 2017. Shadow is designed to assist medical writers and biometrics ... the European Medicines Agency (EMA) in meeting the requirements for de-identifying ... ... ...
(Date:6/23/2017)... ITHACA, N.Y. , June 23, 2017 ... a leader in dairy research, today announced a new ... help reduce the chances that the global milk supply ... this dairy project, Cornell University has become the newest ... Food Supply Chain, a food safety initiative that includes ...
(Date:5/6/2017)... RAM Group , Singaporean based ... in biometric authentication based on a novel  ... to perform biometric authentication. These new sensors are based on ... Ram Group and its partners. This sensor will have ... and security. Ram Group is a next generation ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... ... first-ever genomics analysis platform specifically designed for life science researchers to analyze ... pioneering researcher Rosalind Franklin, who made a major contribution to the discovery ...
(Date:10/11/2017)... ... October 11, 2017 , ... ... will be hosting a Webinar titled, “Pathology is going digital. Is your lab ... digital pathology adoption best practices and how Proscia improves lab economics and realizes ...
(Date:10/11/2017)... ... October 11, 2017 , ... Singh Biotechnology today ... designation to SBT-100, its novel anti-STAT3 (Signal Transducer and Activator of Transcription 3) ... able to cross the cell membrane and bind intracellular STAT3 and inhibit its ...
(Date:10/10/2017)... , Oct. 10, 2017 International research firm Parks ... Strategy, will speak at the TMA 2017 Annual Meeting , October ... trends in the residential home security market and how smart safety and ... Parks ... "The residential ...
Breaking Biology Technology: