Navigation Links
Bioelectronic ‘signatures?hold key to DNA mutations

A new method to identify DNA mutations may shepherd in an era of small, portable, electronic devices for the rapid screening and identification of genes that harbor disease.

Joseph Wang, director of the Center for Bioelectronics and Biosensors at the Biodesign Institute at ASU, led a team effort that successfully merged work in the fields of biosensors, electronics, and nanotechnology to fashion nanocrystals that can act as “DNA biosensors?by electronically recognizing subtle mutations in the DNA. This creates enormous potential for applications such as the diagnosis and treatment of genetic diseases, detection of infectious agents and reliable forensic analysis.

Wang, who recently was recruited to the Biodesign Institute and serves a joint appointment as professor in the chemical and materials department at the Ira A. Fulton School of Engineering and the Department of Chemistry, is a renowned expert in nanomaterial-based biosensors that operate at the scale of a thousand times smaller than the width of a human hair. He wrote 660 papers and has 12 patents to his credit, including involvement in the development of the first noninvasive biosensor for diabetes, the FDA approved Gluco Watch, which monitors glucose levels through human sweat.

“The ultimate goal is to make something similar to a hand-held glucose monitor for future genetic testing,?Wang says. “The electronic detection of DNA is a thing of beauty. You can make it small, low-power, inexpensive and robust.?/p>

Among the keys to unearthing the mysteries behind individual genetic variation and diseases like cancer are fine differences ?single nucleotide polymorphisms, or “SNPs??buried within the 3 billion chemical bases of DNA comprising the human genome. Not every SNP found will necessarily cause a mutation or determine our eye or hair color ?but, on average, SNPs occur about once in every 1,000 DNA bases, adding up to 3 million potential individual differences across the human geno me. Wang’s method allows for an accurate, ultra-sensitive, rapid and low-cost identification of these SNP variants.

“The novelty of the approach is the combination of the nanocrystal tagging of DNA to create electro-diverse signatures and combining them with a fast, portable, low-cost electronic detection,?Wang says.

To achieve the desired results, Wang and his researchers custom-made several individual nanocrystals, known as quantum dots, from four heavy metal salts of lead, cadmium, zinc and copper. Such nanocrystals were selected owing to their ability to yield distinct electronic signatures, with four well-resolved current peaks. Next, the nanocrystals are piggybacked onto individual DNA bases; these DNA bases ?each carrying a single nanocrystal ?bind to a DNA sample and cause minute electrical current changes in the nanocrystal that can be measured with an electrode. The individual base-conjugated nanocrystals are added sequentially to any DNA sample of interest, generating an electronic “fingerprint?that rapidly identifies all possible combinations of SNPs.

Wang’s results recently were featured as the cover story in the March issue of Analytical Chemistry and were published earlier this winter in the Journal of the American Chemical Society. The article can be found on the Web at (http://pubs3.acs.org/acs/journals/doilookup?in_doi=10.1021/ja043780a).


'"/>

Source:Arizona State University


Related biology news :

1. Novel technology detects human DNA mutations
2. Multi-species genome comparison sheds new light on evolutionary processes, cancer mutations
3. New view of cancer: Epigenetic changes come before mutations
4. ASU researchers wire DNA to identify mutations
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:1/21/2017)... , Jan 20, 2017 Research and ... Biometrics Market 2017-2021" report to their offering. ... The global voice recognition biometrics ... 2017-2021. The report covers the present scenario and ... 2017-2021. To calculate the market size, the report considers the revenue ...
(Date:1/18/2017)... -- In vitro diagnostic (IVD) companies were very active in ... Kalorama Information expects that trend to continue – though ... uncertainty in reimbursement and healthcare reform in ... acquisitions landscape. Instead of looking to buy technology, the ... of their home country and also to increase their ...
(Date:1/12/2017)... SAN DIEGO , Jan. 12, 2017  Trovagene, ... circulating tumor DNA (ctDNA) technologies, today announced that it ... Europe and the Middle ... biopsy tests.  This milestone marks the first wave of ... tests for urine and blood samples. The ...
Breaking Biology News(10 mins):
(Date:2/16/2017)... Biotherapeutics (OTCMKTS: IMMG), an early-stage biotechnology company harnessing the power ... triple negative breast cancer (TNBC), announced today their completion of ... The YEi Start in France ... their business in France and Europe.  ... complete an intensive one week immersion in France ...
(Date:2/16/2017)... 2017   Capricor Therapeutics, Inc. (NASDAQ: ... developing first-in-class biological therapies for cardiac and other ... to terminate its license agreement with the Mayo ... Cenderitide. "Our decision to return these ... our efforts to advance our core cell and ...
(Date:2/16/2017)...  Champions Oncology, Inc. (NASDAQ: CSBR ), ... advanced technology solutions and products to personalize the development ... of new cohorts of PDX models to their existing ... Champions, product line in hepatocellular cancer, breast cancer, castrate ... non-small cell lung cancer (including EGFR mutation; ALK/ROS1 positive) ...
(Date:2/16/2017)... ... February 16, 2017 , ... ... industry leading Biochemistry Services specifically targeting the rapidly growing needs of the ... the biochemical and biosimilar characterization , product-related impurity characterization, aggregation ...
Breaking Biology Technology: