Navigation Links
Berkeley researchers lay groundwork for cell version of DNA chip

led Jurkat cells, which are naturally non-adherent. Without the reactive phosphines, there was no detectable attachment of DNA to cell surfaces above background levels.

Once it was established that cell surfaces could be coated with single-stranded DNA, Chandra worked with Douglas to demonstrate that this adhesion system could be used to attach cells to a non-biological surface. Douglas is a student under Mathies, director of UCB's Center for Analytical Biotechnology. Chandra and Douglas used a commercial chemical handle, the sulfur-based thiolate ion, to anchor single-stranded DNA onto gold pads, which were incorporated into microfluidic chips through standard photolithography. After the DNA-coated Jurkat cells were rinsed over the chips, fluorescence microscopy revealed that only those cells coated with single-stranded DNA complementary to the anchored DNA adhered to the gold pads. Cells that were otherwise identical but bearing mismatched DNA sequences were washed away.

"The adhesion of cells is very quick and far stronger than will be needed for most applications," said Chandra. "We just let them incubate for 35 minutes, rinse, and we're ready to go. We've kept our cells alive on the chip for up to 25 hours and the same proportion of cells survive using our method as cells cultured under identical conditions."

The next step, Chandra said, is to develop procedures for making complex arrays of cells quickly. The researchers also need to demonstrate that devices based on this cell-adhesion system will be useful for high-throughput screening applications.


'"/>

Source:DOE/Lawrence Berkeley National Laboratory


Page: 1 2 3 4

Related biology news :

1. Octopuses occasionally stroll around on two arms, UC Berkeley biologists report
2. ORNL, UC Berkeley unravel real-world clues to Earths mysteries
3. UC Berkeley researchers create a biologically-inspired artificial compound eye
4. NYU researchers simulate molecular biological clock
5. Vital step in cellular migration described by UCSD medical researchers
6. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
7. UCSD researchers maintain stem cells without contaminated animal feeder layers
8. Why do insects stop breathing? To avoid damage from too much oxygen, say researchers
9. New protein discovered by Hebrew University researchers
10. First real-time view of developing neurons reveals surprises, say Stanford researchers
11. Agilent Technologies releases automated literature search tool for biology researchers
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Berkeley researchers lay groundwork for cell version DNA chip

(Date:9/19/2014)... fairness did not evolve for the sake of fairness ... of continued cooperation, so say Frans de Waal, PhD, ... about inequity aversion (IA), which is defined as a ... in Science . , Their conclusion comes ... to address their hypothesis that it is the evolution ...
(Date:9/19/2014)... 2014 Nxt-ID, Inc. (Nasdaq: NXTD and ... the growing m-commerce market, updates the "Wocket in Your Pocket" celebrity ... and light middleweight weight classes, Vinny Pazienza (PAZ). ... Game of Thrones actor Ciaran Hinds and ... star Katey Sagal have been cast in Bleed ...
(Date:9/18/2014)... State University researchers have developed a unique method to ... cleanup in rural areas. , The first microbe-powered, ... and quick way to clean up waste from large ... pollution. , Professor Haluk Beyenal and graduate student ... Architecture discuss the system in the online edition of ...
Breaking Biology News(10 mins):Evolution of responses to (un)fairness 2Evolution of responses to (un)fairness 3NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 2NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 3Researchers develop unique waste cleanup for rural areas 2
... laboratory mice that bear aggressive human breast cancers, UC ... growth of tumors -- without damage to surrounding healthy ... issue of the Journal of Nuclear Medicine. , "We ... mice. The next step will be clinical testing in ...
... of hearing have found an underlying molecular cause for ... deafness and the organization of liquid crystals, which are ... the inner ear, sound waves cause the basilar membrane ... trigger nerve impulses that are transmitted to the brain. ...
... in collaboration with colleagues from UC Berkeley and StrataGent ... novel pulsed microjet system engineered to deliver protein drugs ... deeper penetration injection systems cause. The research was published ... of Sciences. , The effort to create needle-free ...
Cached Biology News:UC Davis researchers use heated nanoprobes to destroy breast cancer cells in mice 2Scientists identify molecular cause for one form of deafness 2Researchers design pulsed mircrojet system to deliver protein drugs without pain or bruising 2
(Date:9/20/2014)... Netherlands (PRWEB) September 20, 2014 ... technologies to perform a groundbreaking surgery at the specialized ... the mare's superficial digital flexor tendon was ruptured 90% ... kind of injury is a death sentence for a ... America stem cell treatments . This revolutionary ...
(Date:9/19/2014)... Chips that use light, rather than electricity, to ... and energy efficiency is a growing concern as ... chief components of optical circuits light emitters, ... to build. One promising light source for optical ... optical properties when deposited as a single, atom-thick ...
(Date:9/19/2014)... SAN FRANCISCO , Sept. 19, 2014 /PRNewswire/ ... announced results from preclinical studies characterizing the analgesic ... oral, peripherally-acting kappa opioid receptor agonist molecules. The ... molecule polymer medicinal chemistry platform. The ... described in the medical literature. 1,2 Kappa ...
(Date:9/19/2014)... Cereal grain scientists, quality regulators, ... October 5–8, 2014, for the AACC International (AACCI) ... in Providence, Rhode Island. The comprehensive scientific program ... scientists, chemists, microbiologists, nutritionists, and those interested in ... To date, 1,000 attendees have registered for the ...
Breaking Biology Technology:World’s first tendon replacement using Nanofiber and activated MediVet Stem Cell Fraction 2World’s first tendon replacement using Nanofiber and activated MediVet Stem Cell Fraction 3Toward optical chips 2Toward optical chips 3Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 2Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 3Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 4Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 5Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 2Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 3
... Elekta Neuromag(R),the world-leading equipment for non-invasive ... has been ordered by The,Nebraska Medical Center ... ), With a reputation for excellence, ... Center has earned J.D. Power and Associates,Hospital ...
... response to the,recent comments by certain plaintiff law firms ... (the "Company") (OTC,Bulletin Board: CCYG) asserts that the pending ... so in a court of law. To that end, ... LLP as its counsel and,will defend itself vigorously in ...
... Inc. (Nasdaq: CALP ) today announced that ... Mid Cap Growth Conference in Naples, Florida,will be ... EST. Kevin,Hrusovsky, President and CEO of Caliper, will ... live webcast can be accessed at, http://www.corporate-ir.net/ireye/confLobby.zhtml?ticker=CALP&item_id=17485 ,73. ...
Cached Biology Technology:Elekta Chosen to Deliver Sophisticated Brain Mapping Technology to The Nebraska Medical Center 2Elekta Chosen to Deliver Sophisticated Brain Mapping Technology to The Nebraska Medical Center 3CellCyte Genetics Corp. Continues to Move Its Technology Forward in Light of Plaintiff Law Firm Press Releases 2CellCyte Genetics Corp. Continues to Move Its Technology Forward in Light of Plaintiff Law Firm Press Releases 3
Porcine Coronary Artery Endothelial Cells (PCAEC) (>500,000 cells)...
Proliferin (N-14)...
Recognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts....
... Ligase catalyzes the formation of phosphodiester bonds ... DNAs with 3' hydroxyl and 5' phosphate ... optimizes ligation which can be performed in ... not substrates for this enzyme. A ...
Biology Products: