Navigation Links
Berkeley researchers lay groundwork for cell version of DNA chip

led Jurkat cells, which are naturally non-adherent. Without the reactive phosphines, there was no detectable attachment of DNA to cell surfaces above background levels.

Once it was established that cell surfaces could be coated with single-stranded DNA, Chandra worked with Douglas to demonstrate that this adhesion system could be used to attach cells to a non-biological surface. Douglas is a student under Mathies, director of UCB's Center for Analytical Biotechnology. Chandra and Douglas used a commercial chemical handle, the sulfur-based thiolate ion, to anchor single-stranded DNA onto gold pads, which were incorporated into microfluidic chips through standard photolithography. After the DNA-coated Jurkat cells were rinsed over the chips, fluorescence microscopy revealed that only those cells coated with single-stranded DNA complementary to the anchored DNA adhered to the gold pads. Cells that were otherwise identical but bearing mismatched DNA sequences were washed away.

"The adhesion of cells is very quick and far stronger than will be needed for most applications," said Chandra. "We just let them incubate for 35 minutes, rinse, and we're ready to go. We've kept our cells alive on the chip for up to 25 hours and the same proportion of cells survive using our method as cells cultured under identical conditions."

The next step, Chandra said, is to develop procedures for making complex arrays of cells quickly. The researchers also need to demonstrate that devices based on this cell-adhesion system will be useful for high-throughput screening applications.


'"/>

Source:DOE/Lawrence Berkeley National Laboratory


Page: 1 2 3 4

Related biology news :

1. Octopuses occasionally stroll around on two arms, UC Berkeley biologists report
2. ORNL, UC Berkeley unravel real-world clues to Earths mysteries
3. UC Berkeley researchers create a biologically-inspired artificial compound eye
4. NYU researchers simulate molecular biological clock
5. Vital step in cellular migration described by UCSD medical researchers
6. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
7. UCSD researchers maintain stem cells without contaminated animal feeder layers
8. Why do insects stop breathing? To avoid damage from too much oxygen, say researchers
9. New protein discovered by Hebrew University researchers
10. First real-time view of developing neurons reveals surprises, say Stanford researchers
11. Agilent Technologies releases automated literature search tool for biology researchers
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Berkeley researchers lay groundwork for cell version DNA chip

(Date:7/25/2014)... July 25, 2014 In the first broad-scale estimate of ... scientists and collaborators calculated that trees are saving more ... incidences of acute respiratory symptoms. , While trees, pollution ... less than 1 percent, the impacts of that improvement ... the reduced air pollution at nearly $7 billion every ...
(Date:7/25/2014)... Terra satellite show the different areas that have been affected ... The burn scars show up as reddish-brown splotches of color ... the remote parts of Eastern Russia in the Sakha Republic. ... instrument, it is still possible to see the smoke rising ... below show the devastating number of fires that have plagued ...
(Date:7/25/2014)... and disrupted brain development is an essential part ... largely unknown. However, thanks to funding from the ... University (GW) researcher Anthony-Samuel LaMantia, Ph.D. may be ... to these key questions in the field of ... and physiology at the GW School of Medicine ...
Breaking Biology News(10 mins):First national study finds trees saving lives, reducing respiratory problems 2GW researcher receives grant to answer the how and why of autism during development 2
... Fairbanks, Alaska -- As the Arctic warms, greenhouse gases ... significantly higher levels than previous estimates, according to survey ... 30 issue of the journal Nature. Permafrost thaw ... deforestation, say the authors, but the effect on ...
... of North Carolina at Chapel Hill have shown that it ... an effort to create the ultimate vehicle for gene therapy. ... side effects from using a "chimeric" virus to deliver replacement ... dystrophy. "This trial demonstrates that gene therapy is no ...
... , MONTREAL, November 30, 2011 Take a Petri dish ... distinctive of the toxins that make up the fossil fuel. ... sit for two weeks in an incubator, and surprise, the ... petroleum!" says Mohamed Hijri, a professor of biological sciences and ...
Cached Biology News:Abrupt permafrost thaw increases climate threat 2Clinical trial for muscular dystrophy demonstrates safety of customized gene therapy 2Petroleum-eating mushrooms 2Petroleum-eating mushrooms 3Petroleum-eating mushrooms 4
(Date:7/28/2014)... BRYAN, Texas and COLLEGE STATION, Texas ... hotel theatlashotel.com will be a 177-key, 4-star ... Traditions in Bryan , Texas.  ... As the first hotel within ... will include 177 rooms, a mixture of standard rooms and suites, ...
(Date:7/26/2014)... Albany, NY (PRWEB) July 26, 2014 ... 2020 provides key market data on the Canada ... in millions of US dollars, volume (in units) ... segments - High-End Endoscopy Visualization Systems, Mid-Range Endoscopy ... Browse Full Report at http://www.marketresearchreports.biz/analysis/213254 . , ...
(Date:7/25/2014)... in each person,s digestive tract. Scientists believe that some ... harmful infections, but their role in human health is ... the role of these bacteria, a team of researchers ... fluctuations in the bacterial populations of two research subjects ... July 25 issue of the journal Genome Biology ...
(Date:7/25/2014)... According to a new market report published ... Industry Analysis, Size, Share, Growth, Trends and Forecast, 2014 ... at USD 161,056.5 million in 2014 and is expected ... to 2020, to reach an estimated value of USD ... is witnessing a significant growth due to the increasing ...
Breaking Biology Technology:Texas' Biocorridor Announces the Atlas Hotel, Centerpiece of Mixed Use Community for Biotech Industry 2Texas' Biocorridor Announces the Atlas Hotel, Centerpiece of Mixed Use Community for Biotech Industry 3Texas' Biocorridor Announces the Atlas Hotel, Centerpiece of Mixed Use Community for Biotech Industry 4Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 2Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 3Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 4Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 5Monitoring the rise and fall of the microbiome 2Monitoring the rise and fall of the microbiome 3Biological Drugs Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 2Biological Drugs Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 3
... , , HONG KONG, Dec. ... Company") (NYSE: CO ), China,s leading provider of ... stem cell storage services, today announced that the underwriters of ... portion of their over-allotment option, which will result in the ...
... EMERYVILLE, Calif., Dec. 29 Bionovo, Inc. (Nasdaq: BNVI ... on the anti-tumor mechanism of BN107. The results of ... , describe the potential molecular mechanisms mediating the selective pro-apoptotic ... (ER-) breast cancer cells. , Despite favorable advances that treatment ...
... , , , NEW YORK, Dec. 28 ... New Jersey, recently started using Twitter, the popular social networking tool, as ... new products and beauty trends to his patients and anyone else interested ... my patients on a more personal level. It provides a fun, easy ...
Cached Biology Technology:China Cord Blood Corporation Announces Partial Exercise of Over-Allotment Option 2China Cord Blood Corporation Announces Partial Exercise of Over-Allotment Option 3Bionovo Announces Publication Describing First Novel Dual mTOR Inhibitor, BN107, for the Treatment of Breast Cancer 2Bionovo Announces Publication Describing First Novel Dual mTOR Inhibitor, BN107, for the Treatment of Breast Cancer 3New York Plastic Surgeon Transforms His Practice Using Twitter Status Updates 2New York Plastic Surgeon Transforms His Practice Using Twitter Status Updates 3
5(S)-HETE-d8 is used as an internal standard for the quantification of 5(S)-HETE by stable isotope dilution mass spectrometry....
Full-length cDNA clone CS0DJ013YG01 of T cells (Jurkat cell line) of Homo sapiens (human). [Source:Uniprot/SPTREMBL;Acc:Q86SZ3] Antigen: Recombinant Protein Epitope Signature Tag (PrEST)....
...
...
Biology Products: