Navigation Links
Berkeley researchers lay groundwork for cell version of DNA chip

led Jurkat cells, which are naturally non-adherent. Without the reactive phosphines, there was no detectable attachment of DNA to cell surfaces above background levels.

Once it was established that cell surfaces could be coated with single-stranded DNA, Chandra worked with Douglas to demonstrate that this adhesion system could be used to attach cells to a non-biological surface. Douglas is a student under Mathies, director of UCB's Center for Analytical Biotechnology. Chandra and Douglas used a commercial chemical handle, the sulfur-based thiolate ion, to anchor single-stranded DNA onto gold pads, which were incorporated into microfluidic chips through standard photolithography. After the DNA-coated Jurkat cells were rinsed over the chips, fluorescence microscopy revealed that only those cells coated with single-stranded DNA complementary to the anchored DNA adhered to the gold pads. Cells that were otherwise identical but bearing mismatched DNA sequences were washed away.

"The adhesion of cells is very quick and far stronger than will be needed for most applications," said Chandra. "We just let them incubate for 35 minutes, rinse, and we're ready to go. We've kept our cells alive on the chip for up to 25 hours and the same proportion of cells survive using our method as cells cultured under identical conditions."

The next step, Chandra said, is to develop procedures for making complex arrays of cells quickly. The researchers also need to demonstrate that devices based on this cell-adhesion system will be useful for high-throughput screening applications.


'"/>

Source:DOE/Lawrence Berkeley National Laboratory


Page: 1 2 3 4

Related biology news :

1. Octopuses occasionally stroll around on two arms, UC Berkeley biologists report
2. ORNL, UC Berkeley unravel real-world clues to Earths mysteries
3. UC Berkeley researchers create a biologically-inspired artificial compound eye
4. NYU researchers simulate molecular biological clock
5. Vital step in cellular migration described by UCSD medical researchers
6. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
7. UCSD researchers maintain stem cells without contaminated animal feeder layers
8. Why do insects stop breathing? To avoid damage from too much oxygen, say researchers
9. New protein discovered by Hebrew University researchers
10. First real-time view of developing neurons reveals surprises, say Stanford researchers
11. Agilent Technologies releases automated literature search tool for biology researchers
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Berkeley researchers lay groundwork for cell version DNA chip

(Date:10/16/2014)... Institute of Marine Science have received a 3-year, ... identify the streams and wetlands most vulnerable to ... local governments and citizens conserve these important ecosystems. ... of tidal-marsh observations first gathered by VIMS scientists ... can today,s researchers accurately map the slow but ...
(Date:10/16/2014)... respiratory tract infections and worldwide claims the lives ... and Ghent University have succeeded in developing a ... infection. , Xavier Saelens (VIB/UGent): "We discovered ... for the development of a novel approach to ... in numerous small children and elderly people." , ...
(Date:10/15/2014)... assess the pandemic risk from strains of influenza virus ... allow ourselves to become complacent that the most substantial ... scientists. , Influenza pandemics arise when a new virus ... widespread immunity – spreads in the human population. There ... years, the worst of which – the 1918 Spanish ...
Breaking Biology News(10 mins):EPA grant will help localities conserve headwater wetlands 2EPA grant will help localities conserve headwater wetlands 3New perspectives for development of an RSV vaccine 2Scientists 'must not become complacent' when assessing pandemic threat from flu viruses 2
... A large collaborative study has added to the ... a person will be. The research, published by Cell ... of Human Genetics , identifies uncommon and previously unknown ... the genetic architecture of other complex traits. Although ...
... Most agricultural crops require large quantities of ... for growers is finding ways to balance the ... potentially harmful nitrates that can leach into ground ... "low-input" approaches is challenging researchers to identify genotypes ...
... traditionally relied heavily on hand thinning, a necessary but ... costs and a limited workforce, peach and other stone ... string thinners to minimize the need for hand thinning. ... when it was evaluated in four U.S. growing regions; ...
Cached Biology News:Large-scale analysis identifies new genetic alterations associated with height 2Improving nitrogen use efficiency lessens environmental impact 2Hybrid string blossom thinner tested in peach orchards 2
(Date:10/20/2014)... , Oct. 20, 2014 PureTech , ... tackling big healthcare problems, announced today the closing ... with participation from Invesco Perpetual, a $120 Billion ... to drive PureTech,s existing pipeline forward and to ... has the scientific creativity to really go for ...
(Date:10/20/2014)... 20, 2014 Local veterinary surgeon, Dr. ... investigational study of donor stem cells for dogs with ... therapy and has performed clinical stem cell therapy for ... to determine if a single injection of donor stem ... help reduce pain and inflammation in the treated joints. ...
(Date:10/19/2014)... 19, 2014 NextCODE Health, which enables clinicians and ... real time, today announced the launch of its new genomic ... at the American Society of Human Genetics (ASHG) annual meeting ... sign up and apply for free beta access, visit ... benefits can be viewed here . The ...
(Date:10/19/2014)... (PRWEB) October 19, 2014 The Asia-Pacific ... Analytics market in Asia-Pacific with analysis and forecast of ... million in 2014 to $208 million by 2019, at ... 2014 to 2019. , Browse through the TOC of ... idea of the in-depth analysis provided. It also provides ...
Breaking Biology Technology:PureTech Announces Expansion with Oversubscribed $55M Funding Round 2PureTech Announces Expansion with Oversubscribed $55M Funding Round 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 6Cascade Veterinary Referral Center Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 2The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 2The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 4
... Calif., March 1, 2011 MAP Pharmaceuticals, Inc. (Nasdaq: ... year ended December 31, 2010. The net loss for ... to $13.4 million during the same period in 2009. The net ... compared to $9.0 million for the year ended December 31, 2009. ...
... NBS ), an international biopharmaceutical company with operations ... Smith, the Company,s Chairman and CEO, will present on Tuesday, ... Annual Analyst Conference. The Conference will be held at the ... York. Dr. Smith will present as part of Track 2: ...
... 2011 /PRNewswire-Asia-FirstCall/ -- ShengdaTech, Inc. ("ShengdaTech" or the "Company") ... nano-precipitated calcium carbonate ("NPCC") in China, today announced that ... Anhui Guo, COO and acting CFO and Mr. A. ... & Renshaw Annual China Investment Conference to be held ...
Cached Biology Technology:MAP Pharmaceuticals Reports 2010 Fourth Quarter and Year End Financial Results 2MAP Pharmaceuticals Reports 2010 Fourth Quarter and Year End Financial Results 3MAP Pharmaceuticals Reports 2010 Fourth Quarter and Year End Financial Results 4MAP Pharmaceuticals Reports 2010 Fourth Quarter and Year End Financial Results 5MAP Pharmaceuticals Reports 2010 Fourth Quarter and Year End Financial Results 6MAP Pharmaceuticals Reports 2010 Fourth Quarter and Year End Financial Results 7NeoStem to Present at Wall Street Forum 2011 Financial Conference on Tuesday, March 1 2NeoStem to Present at Wall Street Forum 2011 Financial Conference on Tuesday, March 1 3NeoStem to Present at Wall Street Forum 2011 Financial Conference on Tuesday, March 1 4ShengdaTech to Present at Rodman & Renshaw Annual China Investment Conference in Shanghai 2
Reactivity: Human...
... EasyComp Transformation Kit is designed for ... cells. Advantages over commonly used transformation ... LiCl methods include:- Cells can be made ... - Competent cell preparation and transformation solutions ...
...
... Enhancer System is used in ... optimize PCR of problematic and/or ... Enhancer Solution with 10X PCRX ... specificity broader magnesium concentration broader ...
Biology Products: