Navigation Links
Berkeley researchers lay groundwork for cell version of DNA chip

rough the adaptation of a technique, developed earlier by Bertozzi and her research group, called the "Staudinger ligation." Bertozzi's Staudinger ligation utilizes the sugar complexes called "oligosaccharides" that reside on the surface of every cell, and a reaction between two types of molecules, a nitrogen-containing azide, and a phosphorous-containing phosphine, as a means of attaching probes and reagents to cell surfaces, or to link different molecules inside of the cells.

"The Staudinger ligation has been shown to be both cell friendly and precise, so we exploited the reaction to very specifically link a DNA molecule to the surface of a living cell," said Chandra. "DNA is not normally found on the surfaces of cells, so the ability to deliver the DNA strands, keep them there, and use them for a productive purpose is a significant advance."

Chandra, working under the direction of Francis and Bertozzi, used the Staudinger ligation to create a phosphine-DNA conjugate that makes it possible for the DNA to adhere to a cell's surface. The phosphine-DNA conjugate provides an outer layer of adhesion that is independent of the cell's natural stickiness. As a bonus, the researchers are even able to bind their phosphine-DNA conjugates to cells that are not naturally sticky, providing the first known method for attaching non-adherent cell types to other surfaces. This could prove to be a real advantage.

"For example, it should be possible to array multiple types of non-adherent cells on a chip to create a mini-immune system for evaluating potential therapies," said Chandra.

To test the ability of their phosphine-DNA conjugates to bind to cells, Chandra and his colleagues conducted a series of experiments with human embryonic kidney cells. From their results, they estimated that following treatment, each individual cell was coated with approximately 270,000 DNA molecules. Similar results were obtained using a line of human T-cells, cal
'"/>

Source:DOE/Lawrence Berkeley National Laboratory


Page: 1 2 3 4

Related biology news :

1. Octopuses occasionally stroll around on two arms, UC Berkeley biologists report
2. ORNL, UC Berkeley unravel real-world clues to Earths mysteries
3. UC Berkeley researchers create a biologically-inspired artificial compound eye
4. NYU researchers simulate molecular biological clock
5. Vital step in cellular migration described by UCSD medical researchers
6. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
7. UCSD researchers maintain stem cells without contaminated animal feeder layers
8. Why do insects stop breathing? To avoid damage from too much oxygen, say researchers
9. New protein discovered by Hebrew University researchers
10. First real-time view of developing neurons reveals surprises, say Stanford researchers
11. Agilent Technologies releases automated literature search tool for biology researchers
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Berkeley researchers lay groundwork for cell version DNA chip

(Date:8/19/2014)... Some infants born with neonatal abstinence syndrome ... more difficult time going through withdrawal than others, ... While genetic and epigenetic (when genes are turned ... as potential factors, researchers at Boston University School ... conducted a first of its kind study to ...
(Date:8/19/2014)... today,s crocodiles colonised the seas during warm phases and ... Anglo-French study which establishes a link between marine crocodilian ... period of more than 140 million years. , ... Universit de Lyon, France and formerly from the University ... Nature Communications ., Today, crocodiles are ,cold-blooded, animals that ...
(Date:8/19/2014)... Health Science Center at San Antonio and The University ... awarding of more than $750,000 in grants through the ... Launched in 2003, SALSI is a model of how ... science center join forces for greater research and graduate ... a focus on public health issues and diseases of ...
Breaking Biology News(10 mins):In-utero methadone, Subutex exposure could alter gene expression, cause severe Neonatal Abstience Syndrome 2Evolution of marine crocodilians constrained by ocean temperatures 2San Antonio Life Sciences Institute awards $750,000 to innovators 2San Antonio Life Sciences Institute awards $750,000 to innovators 3
... image of one of the proteins that serves as an on-off ... cell suggests there may be a sort of main power switch ... cells discern light, set the heart racing, or detect pain. ... Nature by a research collaboration involving this year,s Nobel laureates ...
... San Diego Calif. Ludwig researchers Arshad Desai and Christopher ... experiment to parse the molecular details of cell division about ... as a control that, in theory, had no chance of ... Intrigued, Campbell and Desai began exploring how it had defied ...
... a collection of craggy peaks, the highesta mountain known ... soldiers on duty in this mountainous terrain must often ... However, quick climbs without adapting to altitude can lead ... by headache, fatigue, gastrointestinal distress, nausea, and insomnia. ...
Cached Biology News:Structure of cell signaling molecule suggests general on-off switch 2A check on tension 22 days of staging as effective as 4 for high-altitude climbs 22 days of staging as effective as 4 for high-altitude climbs 3
(Date:8/20/2014)... that American Indians largely were wiped out by diseases ... brought to the New World by European explorers., One ... lived in the Americas shortly before Europeans arrived, and ... diseases., But new research led by anthropological geneticists Anne ... the University of Tubingen in Germany indicates the diagnosis ...
(Date:8/20/2014)... 2014  PAREXEL International Corporation (NASDAQ: PRXL ... the Baird Healthcare Conference in New York.  Ingo ... will be making a presentation on PAREXEL and discussing ... 3, 2014. A live webcast of the ... PAREXEL,s website at www.PAREXEL.com in the Upcoming ...
(Date:8/20/2014)... WriteResult®, a leading provider of Electronic Patient Outcome ... myPROpad™ ePRO tablet with the addition of a touchscreen ... widely used and well-respected Patient Reported Outcome (PRO) instruments ... used during clinical trials and in other health care ... to demonstrate Health-Related Quality of Life outcomes (HRQoL) resulting ...
(Date:8/19/2014)... A Geisinger researcher on a personal crusade ... for Disease Control and Prevention (CDC) award recognizing his ... Joseph Boscarino , Ph.D. MPH, senior ... of researchers nominated for the Centers for Disease Control ... Data Methods and Study Design. "Hepatitis C ...
Breaking Biology Technology:Unusual discovery leads to fascinating tuberculosis theory 2Unusual discovery leads to fascinating tuberculosis theory 3PAREXEL International To Present At Baird Healthcare Conference 2EQ-5D™ Assessment Now Available for iPad 2Geisinger investigator nominated for federal award 2Geisinger investigator nominated for federal award 3
... Obstructive ... Sleep Apnea, Advanced ... Employee Productivity; Minimize Litigation Risks, CARLSBAD, Calif., Sept. 5 ... manage was the number,of hours a driver spent behind the wheel. ...
... WEST BRIDGEWATER, Mass., Sept. 5 Pressure,BioSciences, Inc. ... has received,approval to CE Mark the Barocycler NEP2320, ... (PCT) instrument. The,Company also announced that it has ... indicating that the Barocycler NEP2320 has been found ...
... a Proven Leader at Driving Growth, SAN FRANCISCO, ... equity firm that focuses on investments in selected segments,of ... software, today announced the appointment of Michael L. Hurt,to ... Mr. Hurt, CEO,of Altra Industrial Motion (Nasdaq: AIMC ...
Cached Biology Technology:Time For the Trucking and Heavy Machinery Industries to Wake Up! 2Time For the Trucking and Heavy Machinery Industries to Wake Up! 3Time For the Trucking and Heavy Machinery Industries to Wake Up! 4Time For the Trucking and Heavy Machinery Industries to Wake Up! 5Pressure BioSciences, Inc. Announces CE Mark Approval and Compliance with the IEC 61010-1 Standard for a Second PCT-based Instrument, the Barocycler NEP2320 2Pressure BioSciences, Inc. Announces CE Mark Approval and Compliance with the IEC 61010-1 Standard for a Second PCT-based Instrument, the Barocycler NEP2320 3Pressure BioSciences, Inc. Announces CE Mark Approval and Compliance with the IEC 61010-1 Standard for a Second PCT-based Instrument, the Barocycler NEP2320 4Genstar Names Michael Hurt to Its Strategic Advisory Board 2
Attachments: Clip bar for tubes 10 to 19 mm in diameter Overall Dimension Height IC: 6 (15.2) Overall Dimension Length IC: 18.5 (46.9) Overall Dimension Width IC: 4 (10.2)...
BD FastImmune EDTA Solution. Applications: Flow Cytometry Storage Temperature: Refrigerate(2 to 8C)...
... GRADE ,5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside C 14 H ... 6 ,M.W.= 408.63 ,Assay: Greater ... in DMF) ,Biological Activity: Blue color ... Analysis: Agrees with theoretical values ,Thin ...
Antibodies were affinity purified using epitopes specific to RFC1 immobilized on solid support....
Biology Products: