Navigation Links
Berkeley researchers lay groundwork for cell version of DNA chip

rough the adaptation of a technique, developed earlier by Bertozzi and her research group, called the "Staudinger ligation." Bertozzi's Staudinger ligation utilizes the sugar complexes called "oligosaccharides" that reside on the surface of every cell, and a reaction between two types of molecules, a nitrogen-containing azide, and a phosphorous-containing phosphine, as a means of attaching probes and reagents to cell surfaces, or to link different molecules inside of the cells.

"The Staudinger ligation has been shown to be both cell friendly and precise, so we exploited the reaction to very specifically link a DNA molecule to the surface of a living cell," said Chandra. "DNA is not normally found on the surfaces of cells, so the ability to deliver the DNA strands, keep them there, and use them for a productive purpose is a significant advance."

Chandra, working under the direction of Francis and Bertozzi, used the Staudinger ligation to create a phosphine-DNA conjugate that makes it possible for the DNA to adhere to a cell's surface. The phosphine-DNA conjugate provides an outer layer of adhesion that is independent of the cell's natural stickiness. As a bonus, the researchers are even able to bind their phosphine-DNA conjugates to cells that are not naturally sticky, providing the first known method for attaching non-adherent cell types to other surfaces. This could prove to be a real advantage.

"For example, it should be possible to array multiple types of non-adherent cells on a chip to create a mini-immune system for evaluating potential therapies," said Chandra.

To test the ability of their phosphine-DNA conjugates to bind to cells, Chandra and his colleagues conducted a series of experiments with human embryonic kidney cells. From their results, they estimated that following treatment, each individual cell was coated with approximately 270,000 DNA molecules. Similar results were obtained using a line of human T-cells, cal
'"/>

Source:DOE/Lawrence Berkeley National Laboratory


Page: 1 2 3 4

Related biology news :

1. Octopuses occasionally stroll around on two arms, UC Berkeley biologists report
2. ORNL, UC Berkeley unravel real-world clues to Earths mysteries
3. UC Berkeley researchers create a biologically-inspired artificial compound eye
4. NYU researchers simulate molecular biological clock
5. Vital step in cellular migration described by UCSD medical researchers
6. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
7. UCSD researchers maintain stem cells without contaminated animal feeder layers
8. Why do insects stop breathing? To avoid damage from too much oxygen, say researchers
9. New protein discovered by Hebrew University researchers
10. First real-time view of developing neurons reveals surprises, say Stanford researchers
11. Agilent Technologies releases automated literature search tool for biology researchers
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Berkeley researchers lay groundwork for cell version DNA chip

(Date:4/21/2015)... BURNABY, Canada , April 21, 2015 ... spending and evolving government policies are boosting access control ...   According to a recently published report ... & Opportunities, 2020 ", the access control systems market ... to nearly USD1.2 billion by 2020.The access control systems ...
(Date:4/20/2015)... PASADENA, Calif. , April 20, 2015 ... San Gabriel Valley to implant a new miniaturized, wireless ... HF System is the first and only FDA-approved heart ... reduce hospital admissions when used by physicians to manage ... a sensor that is implanted in the pulmonary artery ...
(Date:4/20/2015)... , April 20, 2015 The ... Glenbeigh Records Management (GRM), Ireland,s ... Dubai Islamic Bank. Having built up an impressive track record ... a leading player within the records management sector in ... to double is GCC staffbase and employ a further eight ...
Breaking Biology News(10 mins):Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 2Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 3Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3
... the next big innovation in sustainable climate technology? ... for Business Law and Regulation at Case Western Reserve ... do just that. "Technology-induced prizes have a long ... on a Climate Prize Rewarding Energy Innovation to Achieve ...
... ST. AUGUSTINE, TRINIDAD AND TOBAGOFlower color in plants ... carotenoids. Research has found that the ultimate color displayed ... also on other factors, including cell shape, presence of ... role of pH in creating color has allowed plant ...
... Ill. Researchers have identified a protein long known to ... growth. Their study, in the journal Oncogene , is ... accomplishes this feat. "People suggested that Runx3 might be ... it is down-regulated in a lot of breast cancer cell ...
Cached Biology News:CWRU law professor eyes prize-based incentives to generate climate innovation 2Engineering blue-hued flowers 2Team identifies new breast cancer tumor suppressor and how it works 2
(Date:4/21/2015)... discovery company, Novogen, today announced that its subsidiary joint ... received notification from the U.S. Food and Drug Administration ... granted Orphan Drug Designation for ovarian cancer. ... experimental drug and has been instigated in a number ... and Australia to encourage ...
(Date:4/21/2015)... 21, 2015 Simply “trying” to ensure ... is not enough, the European Commission’s Digital Agenda (DG ... ensuring that digital technologies help deliver the growth which ... will help ensure that women’s voices are heard ... the international society for optics and photonics , are ...
(Date:4/21/2015)... NEW YORK , April 21, 2015  This ... acquisition deals in the pharmaceuticals and biotechnology industry. The ... value. Trends are also observed based on the geographic ... nature of cross-border deals and takes into account various ... stock premium rise of the target company. ...
(Date:4/21/2015)... , April 21, 2015 The North American ... of 5.8% from 2014 to 2019. Based on geography, this ... , and Mexico . The U.S. ... Canada . The major factors driving the growth of ... industry. These industries are the prime users of chromatography techniques. ...
Breaking Biology Technology:Cantrixil Receives Orphan Drug Designation from FDA 2Cantrixil Receives Orphan Drug Designation from FDA 3Cantrixil Receives Orphan Drug Designation from FDA 4Cantrixil Receives Orphan Drug Designation from FDA 5Just to try is not enough, say organizations working to promote women in science and technology 2Just to try is not enough, say organizations working to promote women in science and technology 3Funding Patterns in the Global Pharma and Biotech Industry 2North American Chromatography systems Market by Types, & by End-Users - Forecast to 2019 2North American Chromatography systems Market by Types, & by End-Users - Forecast to 2019 3
... ET; 8:00 a.m. PT, VANCOUVER and SAN DIEGO, ... a clinical-stage developer of drugs for infectious,diseases, will host ... at 11:00 a.m. ET (8:00 a.m. PT) to discuss ... 2008 and provide an update on,company activities. To participate ...
... on Detroit Area, DETROIT, March 11 ... its new study, Affordable Broadband:,Empowering Communities Across the ... to education, health care and civic,involvement in minority ... Empowerment Council (DEC) that was launched,earlier this year ...
... March 11 Haemonetics,Corporation announced today the appointment ... Chief Marketing Officer. In this position, Mr. Allen,will ... portfolio in addition to his current responsibilities as ... commented, "Pete Allen was an,architect of our strategic ...
Cached Biology Technology:Study Finds Broadband Access Key to Empowerment of Minority Communities 2Haemonetics(R) Appoints Peter Allen as Chief Marketing Officer 2
... Disrupts biological cells for DNA ... 96 well plates (includes 96 well ... consistent lysis across the entire plate ... wells). Can be used for ...
Request Info...
GBL Antibody...
Dnase and Rnase free...
Biology Products: