Navigation Links
Baumann Lab defines proteins that distinguish chromosome ends from DNA double-strand breaks

Peter Baumann, Ph.D., Assistant Investigator, and Nancy Bae, Ph.D., Postdoctoral Research Associate in the Baumann Lab, have published a paper offering insight into the way cells protect chromosome ends from misguided repair.

Published in tomorrow's issue of Molecular Cell, their paper entitled "A RAP1/TRF2 Complex Inhibits Non-Homologous End Joining at Human Telomeric DNA Ends" employed a biochemical assay for double-strand break repair to define the minimal requirements for the protection of telomeric DNA at the ends of chromosomes.

"Surprisingly, we found that neither long single-stranded overhangs nor t-loop formation is essential to prevent illegitimate repair of telomeric ends," said Dr. Bae. "Instead, a short tandem array of telomeric repeats bound by a Rap1/Trf2 complex is sufficient to impede non-homologous end joining in a highly directional manner."

It has long been understood that chromosome ends are distinct from DNA double-strand breaks and that the cellular machinery that repairs DNA breaks does not act on telomeres. But how repair factors are prevented from acting at chromosome ends has been a hotly debated issue. Over the past decade, several telomeric complexes and structures have been identified and proposed to protect chromosome ends, but conclusive evidence that any of these are required for protection has been lacking.

"We set out to define the minimal requirements that would allow the DNA repair machinery to distinguish a chromosome end from a break," said Dr. Baumann. "By establishing an in vitro assay for chromosome end protection and by implicating specific proteins, we have opened the door to elucidate the mechanism by which RAP1/TRF2 inhibits double-strand break repair at chromosome ends."

"These findings are important for establishing a better understanding of tumor development," said Robb Krumlauf, Ph.D., Scientific Director. "Genomic instability and gross chromosomal rearrangements are a hallmark of cancer cells. The mechanisms that initiate and drive these events are only poorly understood, but it is widely accepted that loss of chromosome end protection can initiate genomic instability through bridge-breakage-fusion cycles. It is, therefore, very important to understand the mechanism of chromosome end protection and how and why it fails during tumorigenesis."


'"/>

Source:Stowers Institute for Medical Research


Related biology news :

1. Study defines effective microbicide design for HIV/AIDS prevention
2. New, automated tool successfully classifies and relates proteins in unprecedented way
3. UWs Rosetta software to unlock secrets of many human proteins
4. Global analysis of membrane proteins
5. UNC plant researchers discover proteins interact to form hair-trigger protection against invaders
6. Mad cow proteins successfully detected in blood
7. Researchers develop new method for facile identification of proteins in bacterial cells
8. Hopkins scientists uncover tags that force proteins to cell surface
9. Researchers create functioning artificial proteins using natures rules
10. UCSD discovery may provide novel method to generate medically useful proteins
11. Prostate cancer uses Wnt signaling proteins to promote growth of bone tumors
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/11/2017)... Florida , April 11, 2017 ... a security technology company, announces the appointment of independent Directors ... Bendheim to its Board of Directors, furthering the company,s ... ... of NXT-ID, we look forward to their guidance and benefiting ...
(Date:4/5/2017)... , April 4, 2017 KEY FINDINGS ... anticipated to expand at a CAGR of 25.76% during ... diseases is the primary factor for the growth of ... report: https://www.reportbuyer.com/product/4807905/ MARKET INSIGHTS The global ... product, technology, application, and geography. The stem cell market ...
(Date:3/30/2017)... March 30, 2017 The research team of ... three-dimensional (3D) fingerprint identification by adopting ground breaking 3D fingerprint minutiae ... realm of speed and accuracy for use in identification, crime investigation, ... cost. ... A research ...
Breaking Biology News(10 mins):
(Date:10/10/2017)... , ... October 10, 2017 , ... Dr. Bob Harman, ... his local San Diego Rotary Club. The event entitled “Stem Cells ... and had 300+ attendees. Dr. Harman, DVM, MPVM was joined by two human ...
(Date:10/10/2017)... ... October 10, 2017 , ... The Pittcon Program Committee ... honoring scientists who have made outstanding contributions to analytical chemistry and applied ... the world’s leading conference and exposition for laboratory science, which will be held ...
(Date:10/9/2017)... ... October 09, 2017 , ... The Giving Tree Wellness Center ... the needs of consumers who are incorporating medical marijuana into their wellness and ... , As operators of two successful Valley dispensaries, The Giving Tree’s two founders, ...
(Date:10/7/2017)... 6, 2017  The 2017 Nobel Prize in ... Jacques Dubochet, Joachim Frank and ... cryo-electron microscopy (cryo-EM) have helped to broaden ... biology community. The winners worked with systems manufactured ... produce highly resolved, three-dimensional images of protein structures ...
Breaking Biology Technology: