Navigation Links
Basis for DNA ejection from single phage particles

Studying phage, a primitive class of virus that infects bacteria by injecting its genomic DNA into host cells, researchers have gained insight into the driving force behind this poorly understood injection process, which has been proposed in the past to occur through the release of pressure accumulated within the viral particle itself.

Almost all phages (also known as bacteriophages) are formed of a capsid structure, or head, in which the viral genome is packaged during morphogenesis, and a tail structure that ensures the attachment of the phage to the host bacteria. A common feature of phages is that during infection, only their genome is transferred to the bacterial host's cytoplasm, whereas the capsid and tail remain bound to the cell surface. This situation is very different from that found in most eukaryotic viruses, including those that infect humans, in that the envelope of these viruses fuses with the host plasma membrane so that the genome is delivered without directly contacting the membrane.

Phage nucleic acid transport poses a fascinating biophysical problem: Transport is unidirectional and linear; it concerns a unique molecule the size of which may represent 50 times that of the bacterium. The driving force for DNA transport is still poorly defined. It was hypothesized that the internal pressure built during packaging of the DNA in the phage capsid was responsible for DNA ejection. This pressure results from the condensation of the DNA during morphogenesis ?for example, another group recently showed that the pressure at the final stage of encapsulation for a particular bacteriophage reached a value of 60 atomospheres, which is close to ten times the pressure inside a bottle of champagne. In the new work reported this week, researchers have evaluated whether the energy thus stored is sufficient to permit phage DNA ejection, or only to initiate that process.

The researchers used fluorescently labeled phage DNA to investigate in real time (and with a resolution time of 750 milliseconds) the dynamics of DNA ejection from single phages. The ejected DNA was measured at different stages of the ejection process after being stretched by applied hydrodynamic flow. The study demonstrated that DNA release is not an all-or-none process, but rather is unexpectedly complex. DNA release occurred at a very high rate, reaching 75,000 base pairs of DNA/sec, but in a stepwise fashion. Pausing times were observed during ejection, and ejection was transiently arrested at definite positions of the genome in close proximity to genetically defined physical interruptions in the DNA. The authors discuss the relevance of this stepwise ejection to the transfer of phage DNA in vivo.

###

Stéphanie Mangenot, Marion Hochrein, Joachim Rädler, and Lucienne Letellier: "Real-Time Imaging of DNA Ejection from Single Phage Particles"

The other members of the research team include Stéphanie Mangenot of the Laboratoire de Physique des Solides, UMR CNRS at Université Paris Sud; Marion Hochrein and Joachim Rädler of Ludwig Maximilian Universität; and Lucienne Letellier of the Institut de Biochimie et Biophysique Moléculaire et Cellulaire, UMR, CNRS at Université Paris Sud. This project was supported in part by the Centre National de la Recherche Scientifique program "Dynamique et Réactivité des Assemblages Biologiques" and by SFB 563 "Bioorganic Functional Systems on Solids."

Publishing in Current Biology, Volume 15, Number 5, March 8, 2005, pages 430-435. http://www.current-biology.com


'"/>

Source:Cell Press


Related biology news :

1. Fibril Shape Is The Basis Of Prion Strains And Cross-species Prion Infection
2. New type of rejection blocker protects kidneys after transplant
3. Simple sea sponge helps scientists understand tissue rejection
4. Researchers identify genes associated with lung transplant rejection
5. Carnegie Mellon develops non-invasive technique to detect transplant rejection at cellular level
6. Combination therapy with a monocloncal antibody and a vaccine leads to tumor rejection
7. Proteins may predict lung transplant rejection
8. Opposing fat metabolism pathways triggered by a single gene
9. Ancient DNA confirms single origin of Malagasy primates
10. No single gene for ageing
11. Natural selection at single gene demonstrated
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/14/2016)... , Allemagne, March 14, 2016 ... http://www.apimages.com ) - --> - Renvoi : ... - --> --> ... solutions biométriques, fournit de nouveaux lecteurs d,empreintes digitales ... LF10 de DERMALOG sera utilisé pour produire des ...
(Date:3/9/2016)... YORK , March 9, 2016 This ... and future states of the RNA Sequencing (RNA Seq) ... segments such as instruments, tools and reagents, data analysis, ... Analyze various segments of the RNA-Sequencing market such as ... services Identify the main factors affecting each segment and ...
(Date:3/3/2016)... , March 3, 2016  2016FLEX, organized ... this week highlighting advancements in flexible, hybrid and ... record setting attendance - have gathered for short ... fast-growing field of electronics. The Flex Conference celebrates ... point for companies, R&D organizations, and universities contributing ...
Breaking Biology News(10 mins):
(Date:4/29/2016)... ... April 30, 2016 , ... The MIT bioLogic design team has ... team explored how bacterial properties can be applied to fabric and formed into living ... move in response to humidity change. The team harvested Natto cells and applied them ...
(Date:4/29/2016)... ... 2016 , ... Amendia, Inc., a leading designer, developer, manufacturer ... completion of a significant transaction and partnership that positions Amendia for accelerated growth ... Kohlberg & Company, L.L.C. (“Kohlberg”), a leading private equity firm specializing in middle ...
(Date:4/29/2016)... ... April 29, 2016 , ... ... to transform technology into a viable company, CereScan’s CEO, John Kelley, joined other ... a recognized leader and mentor in the Denver area business community, shared his ...
(Date:4/29/2016)... ... , ... Intelligent Implant Systems announced today that the two-level components for the ... United States. These components expand the capabilities of the system and allow Revolution™ ... October of 2015, the company has seen significant sales growth in 1Q 2016, and ...
Breaking Biology Technology: