The combination of the GeneChip Blood RNA Concentration Kit (featuring the PAXgene Blood RNA System) and the GeneChip Globin-Reduction RNA Controls standardizes all the steps of whole blood RNA processing -- from sample stabilization to analysis on GeneChip microarrays. This has been a major challenge in expression studies of whole blood, as prior RNA preparation methods have often yielded expression results with variable levels of sensitivity.
Use of the Globin-Reduction Protocol and associated reagent kits enables researchers to detect subtle changes in gene expression by reducing the overwhelming amounts of globin messenger RNA (mRNA) present in whole blood. Large amounts of globin mRNA can significantly interfere with gene expression assay results. High globin mRNA levels contributed from a fraction of the red blood cells have typically masked the less abundant, but more important, changes of gene expression associated with white blood cells, such as lymphocytes and monocytes. Counteracting the effects of globin mRNA during target preparation significantly increases assay sensitivity on GeneChip arrays.
The GeneChip Globin Reduction Protocol links PreAnalytiX' already proven standard for whole blood collection, stabilization and isolation of RNA with Affymetrix' leading product line for microarray-based analysis of whole blood cellular RNA profiles.
"An accurate picture of in vivo gene expression profiles is essential for understanding gene regul