Navigation Links
'Accelerated evolution' converts RNA enzyme to DNA enzyme in vitro

lecular biology-that the transfer of sequential genetic information proceeds from nucleic acid to nucleic acid, and from nucleic acid to protein. But a far different situation exists with regard to the transfer of catalytic function, which does not occur sequentially in contemporary biology. The new study shows that catalytic function can be transmitted sequentially between two different nucleic acid-like molecules, suggesting how it might have been conveyed from pre-RNA molecules to RNA during the simpler pre-RNA world period.

There are several candidates for the initial pre-RNA molecule, all of which have the ability to form base-paired structures with themselves and with RNA. Cross-pairing would allow genetic information to be transferred from these pre-RNA molecules to RNA. The catalytic function of these early enzymes might have been transferred to a corresponding RNA enzyme following the acquisition of a few critical mutations, the study said, just as the evolutionary change of a ribozyme to a deoxyribozyme with the same or similar catalytic functions might also have occurred through random mutation and selection.

For the study, an RNA ribozyme was converted to a corresponding deoxyribozyme through in vitro evolution. The ribozyme was first prepared as a DNA molecule of the same RNA sequence but with no detectable catalytic activity. A large number of randomized variations of this DNA were prepared, and repeated cycles of in vitro evolution were carried out. The result was a deoxyribozyme with about the same level of catalytic activity as the original ribozyme.

"The use of in vitro evolution provides the means to convert a ribozyme to a corresponding deoxyribozyme rapidly," Joyce said. "In the laboratory these procedures allow us to carry out many generations of test tube evolution. The resulting molecules have interesting catalytic properties, they teach us something new about evolution, and they have potential application as ther
'"/>

Source:Scripps Research Institute


Page: 1 2 3

Related biology news :

1. On a flys wing, scientists tally evolutions winners and losses
2. Taking evolutions temperature: Researchers pinpoint the energy it takes to make a species
3. Mobile DNA part of evolutions toolbox
4. Gene therapy converts dead bone graft to new, living tissue
5. Protecting virus offers instant flu protection and converts flu infections into their own vaccines
6. Lack of enzyme turns fat cells into fat burners
7. Scientists find missing enzyme for tuberculosis iron scavenging pathway
8. Researchers report new pro-inflammatory role for anti-inflammatory enzyme
9. Purdue researchers use enzyme to clip DNA wires
10. Scientists take aim at virulent bacteria by decoding machinery of key control enzyme
11. VCU Massey Cancer Center study shows enzyme linked to spread of breast cancer cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Accelerated evolution converts RNA enzyme DNA enzyme vitro

(Date:12/17/2014)... , Dec. 15, 2014 Research and Markets ... addition of the "Samsung Galaxy S5 - ... report to their offering. ... totally different sensing technology than the iPhone 5S, ... sensor in its product. The Galaxy ...
(Date:12/17/2014)... , Dec. 16, 2014  Automation is fundamentally ... more evident than at international borders. Over the ... scanners have allowed veteran travelers to self process ... Control (APC) Kiosks at an increasing number of ... According to Maxine Most ...
(Date:12/11/2014)... PAUL, Minn. , Dec. 10, 2014 /PRNewswire/ ... wireless physiologic monitoring, has released a new series ... needs of preclinical toxicology researchers. M series, part ... help toxicologists collect the best possible physiologic data ... Adding functional endpoints to toxicology studies ...
Breaking Biology News(10 mins):Samsung Galaxy S5 - Home Button Synaptics Fingerprint Sensor Technology Analysis 2Automated Border Control (ABC) Transforms the Global Travel Experience With More Than 2500 ABC eGates and APC Kiosks Deployed In Airports, Seaports, and Land Borders Worldwide 2New telemetry implants expected to change how large animal toxicology studies are conducted 2
... in <A HREF=",http://chinese.eurekalert.org/zh/pub_releases/2009-05/sfeb-cai052609.php,">Chinese . Researchers ... expression of a form of cytochrome P-450 (CYP4B1) ... in mice by caffeic acid, an anti-inflammatory antioxidant ... which appear in the June 2009 issue of ...
... a soldier is wounded during combat, surgeons must focus ... Technologies that could improve the repair and regeneration processes ... but they are not being moved quickly enough into ... Georgia Tech Center for Advanced Bioengineering for Soldier Survivability ...
... by millions worldwide to effectively lower blood cholesterol levels, ... (MS) patients treated with high daily dosages. A ... (MNI), McGill University, demonstrates that statin therapy in mice ... system. The findings, published in The American Journal ...
Cached Biology News:Caffeic acid inhibits colitis in a mouse model -- is a drug-metabolizing gene crucial? 2New center at Georgia Tech aims to improve recovery of soldiers with severe injuries 2New center at Georgia Tech aims to improve recovery of soldiers with severe injuries 3One size does not fit all 2
(Date:12/19/2014)... (PRWEB) December 18, 2014 ... nation’s leading specialty pharmacies, has an expanded team ... clinical pharmacy consultants for prescriber offices in their ... , “We have a variety of Regional ... example, those specializing in hepatitis C help prescribers ...
(Date:12/19/2014)... 18, 2014 Reports from CDC ... States suffer from one or more chronic health conditions ... Medication can address some of these diseases, but may ... many sufferers face. While implanted nerve stimulation devices today ... whole nerve, which can induce side effects. , ...
(Date:12/19/2014)... With only 2 weeks left of ... Sartorius Biohit products . The product specials include Buy ... Pipette Trade-in Program, and free pipette tips. , Sartorius ... the world. They are ideal for lab technicians performing ... ,     Full volume range of 0.1 uL to ...
(Date:12/19/2014)... NEW YORK , Dec. 18, 2014 ... novel therapies for the treatment of chronic pain, announced ... held today. "This was a transformational year ... a promising future for our company," said Sergio ... past year, we have executed on key financial, human ...
Breaking Biology Technology:BioPlus Specialty Pharmacy Expands ‘Regional Pharmaceutical Care Liaison’ Team 2BioPlus Specialty Pharmacy Expands ‘Regional Pharmaceutical Care Liaison’ Team 3Draper Wins GSK Funding for Smaller, Smarter Implantable Devices 2Major Pipette Distributor Pipette.com Announces Sartorius Year End Specials 2Relmada Therapeutics Announces Results of Annual Shareholders Meeting 2
... Help Drive Production Efficiency and Reliability for New Shire ... HON ) today announced it ... research and development capabilities for creating life-saving medications. Shire ... plc, will use several Honeywell solutions - including ...
... the world renowned Japanese Asahi Breweries announced a new partnership, which ... Japan between Mitsui and Asahi. , ... Lexington, KY ... world renowned Japanese Asahi Breweries announced a new partnership, which will ...
... 11 VERIDIAM ( www.veridiam.com ), a major ... and assemblies, custom engineered to meet the needs ... other highly-critical applications, today announced the appointment of ... Gale will head up operations of all Veridiam ...
Cached Biology Technology:Honeywell Technology Selected to Control Manufacturing Processes at Major U.S. Biotech Site 2Honeywell Technology Selected to Control Manufacturing Processes at Major U.S. Biotech Site 3Honeywell Technology Selected to Control Manufacturing Processes at Major U.S. Biotech Site 4Alltech, Mitsui and Asahi Breweries Create New Venture 2Alltech, Mitsui and Asahi Breweries Create New Venture 3Veridiam Appoints Andrew Gale as Chief Executive Officer 2
Chicken polyclonal to UCK2 ( Abpromise for all tested applications). Antigen: Full length protein, corresponding to amino acids 1-261 of Human UCK2 Entrez Gene ID: 7371 Swiss Protein ID: ...
Thermo Labsystems offers a basic reader for 96-well microplates that is especially adapted for ELISA and other colorimetric assays....
Abgene's ALTO-8 is the first automated decapper/capper for Abgene Twist-Lock tubes....
Abgene's ALTO-8 is the first automated decapper/capper for Abgene Twist-Lock tubes....
Biology Products: