Navigation Links
'Accelerated evolution' converts RNA enzyme to DNA enzyme in vitro

This "evolutionary conversion" provides a modern-day snapshot of how life as we understand it may have first evolved out of the earliest primordial mix of RNA-like molecules-sometimes referred to as the "pre-RNA world"-into a more complex form of RNA-based life (or the "RNA world") and eventually to cellular life based on DNA and proteins. Nucleic acids are large complex molecules that store and convey genetic information, but can also function as enzymes.

While the transfer of sequence information between two different classes of nucleic acid-like molecules-between RNA and DNA, for example-is straightforward because it relies on the one-to-one correspondence of the double helix pairing, transferring catalytic function is significantly more difficult because function cannot be conveyed sequentially. The present study demonstrates that the "evolutionary conversion" of an RNA enzyme to a DNA enzyme with the same function is possible, however, through the acquisition of a few critical mutations.

The study was released in an advance online version of the journal Chemistry & Biology.

Scripps Research Professor Gerald F. Joyce, a member of the Skaggs Institute for Chemical Biology whose laboratory conducted the study, said, "During early life on earth both genetic information and catalytic function were thought to reside only in RNA. In our study, the evolutionary transition from an RNA to a DNA enzyme represents a genuine change, rather than a simple expansion, of the chemical basis for catalytic function. This means that similar evolutionary pathways may exist between other classes of nucleic acid-like molecules. These findings could help answer some fundamental questions concerning the basic structure of life and how it evolved over time."

As Francis Crick, the Nobel laureate who, along with James Watson uncovered the double helix structure of DNA, articulated in 1970, all known organisms operate according to the central dogma of mo lecular biology-that the transfer of sequential genetic information proceeds from nucleic acid to nucleic acid, and from nucleic acid to protein. But a far different situation exists with regard to the transfer of catalytic function, which does not occur sequentially in contemporary biology. The new study shows that catalytic function can be transmitted sequentially between two different nucleic acid-like molecules, suggesting how it might have been conveyed from pre-RNA molecules to RNA during the simpler pre-RNA world period.

There are several candidates for the initial pre-RNA molecule, all of which have the ability to form base-paired structures with themselves and with RNA. Cross-pairing would allow genetic information to be transferred from these pre-RNA molecules to RNA. The catalytic function of these early enzymes might have been transferred to a corresponding RNA enzyme following the acquisition of a few critical mutations, the study said, just as the evolutionary change of a ribozyme to a deoxyribozyme with the same or similar catalytic functions might also have occurred through random mutation and selection.

For the study, an RNA ribozyme was converted to a corresponding deoxyribozyme through in vitro evolution. The ribozyme was first prepared as a DNA molecule of the same RNA sequence but with no detectable catalytic activity. A large number of randomized variations of this DNA were prepared, and repeated cycles of in vitro evolution were carried out. The result was a deoxyribozyme with about the same level of catalytic activity as the original ribozyme.

"The use of in vitro evolution provides the means to convert a ribozyme to a corresponding deoxyribozyme rapidly," Joyce said. "In the laboratory these procedures allow us to carry out many generations of test tube evolution. The resulting molecules have interesting catalytic properties, they teach us something new about evolution, and they have potential application as ther apeutic and diagnostic agents."


'"/>

Source:Scripps Research Institute


Related biology news :

1. On a flys wing, scientists tally evolutions winners and losses
2. Taking evolutions temperature: Researchers pinpoint the energy it takes to make a species
3. Mobile DNA part of evolutions toolbox
4. Gene therapy converts dead bone graft to new, living tissue
5. Protecting virus offers instant flu protection and converts flu infections into their own vaccines
6. Lack of enzyme turns fat cells into fat burners
7. Scientists find missing enzyme for tuberculosis iron scavenging pathway
8. Researchers report new pro-inflammatory role for anti-inflammatory enzyme
9. Purdue researchers use enzyme to clip DNA wires
10. Scientists take aim at virulent bacteria by decoding machinery of key control enzyme
11. VCU Massey Cancer Center study shows enzyme linked to spread of breast cancer cells
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/22/2017)... -- With the biometrics market to exceed $10 ... that innovative and agile startups must incorporate into ... changing competitive landscape: multifactor authentication (MFA), point-of-sale (PoS), ... "Companies can no longer afford to cut corners ... Pavlakis , Industry Analyst at ABI Research. "Pairing ...
(Date:2/13/2017)... , Feb. 13, 2017  RSA Conference -- RSA, ... that is designed to enhance fraud detection and ... in the RSA Fraud & Risk Intelligence Suite. ... to leverage additional insights from internal and external ... better protect their customers from targeted cybercrime attacks. ...
(Date:2/8/2017)... (NASDAQ: AWRE ), a leading supplier of biometrics ... and year ended December 31, 2016. Revenue ... to $6.9 million in the same quarter last year. Operating ... compared to $2.6 million in the fourth quarter of 2015. ... million, or $0.02 per diluted share, which compares to $1.8 ...
Breaking Biology News(10 mins):
(Date:3/22/2017)... , March 22, 2017 MarketNewsUpdates.com ... ... cancer conditions are being pressured as of late due to ... cancer pain management has a dramatic impact on patient,s quality ... and development activities for identifying new forms of opioid formulations ...
(Date:3/22/2017)... Freeport, Grand Bahama (PRWEB) , ... March 21, 2017 , ... ... a free educational seminar as part of their live events series, “Stem Cell Therapy: ... adult stem cell facility under the 2013 Stem Cell Research and Therapy Act, ...
(Date:3/22/2017)... Wilmington, MA (PRWEB) , ... ... ... Technology Group ( WMFTG ) has unveiled its innovative Quantum peristaltic pump ... in peristaltic innovation, Quantum sets the new standard for high-pressure feed pumps ...
(Date:3/22/2017)... 2017  Personal Genome Diagnostics Inc. (PGDx) today ... with the U.S. Department of Veterans Affairs (VA) ... the company,s new CancerSELECT ™ 125 test. ... profiling test that includes microsatellite instability status (MSI), ... to checkpoint inhibitor immunotherapies. CancerSELECT 125 will be ...
Breaking Biology Technology: