Navigation Links
A wolf in sheep's clothing: Plague bacteria reveal one of their virulence tricks

The bacterium that causes the plague belongs to a virulent family of bacteria called Yersinia, a group that also includes a pathogen responsible for food poisoning. These bacteria insert into their host cells proteins and other virulence factors, which kill by -- among other things -- disrupting the cells' normal structure. One of these proteins, called YpkA, attacks a cell's internal skeleton. Now, a study published by Rockefeller University researchers in the most recent issue of Cell shows exactly how YpkA does this, proving the protein's mechanism from the atomic to the organismal level and providing a potential target for new antibiotic drugs.

C. Erec Stebbins, associate professor and head of the Laboratory of Structural Microbiology, and graduate student Gerd Prehna solved the structure for one region of the YpkA protein, a "binding domain" where it interlocks with another protein on the host cell's membrane. By looking at the crystal structure of this protein-protein complex, Prehna discovered that the configuration looked just like one formed by some of the host's own signaling proteins. And it's this mimicry, he found, that leads to a signaling shutdown and deregulation of the cell's normal structure.

After establishing this effect, Prehna set about disrupting it by mutation. Using the structure to guide him, he changed three amino acids of YpkA that contacted host proteins, and then looked at how the mutated bacteria affected human cells compared to the original wild-type Yersinia. His results confirmed the hypothesis from the structural study: While the wild-type YpkA wreaked havoc on their host cells' cytoskeletons, the mutant left the actin-based skeleton intact.

Then, the researchers took it one step further. Stebbins and Prehna worked with collaborators at Stony Brook University, who created Yersinia bacteria with Prehna's mutations. The Stony Brook researchers then injected mice with the wild-type and mutant strains of Yersini a. All the mice infected with the wild-type bacteria died within nine days of exposure. But the group that received the YpkA mutant had an 80 percent survival rate, showing that Prehna's mutation drastically lowered Yersinia's harmful effects. "Altering this binding site not only impairs the bacteria's ability to disrupt the host cytoskeleton," Stebbins says, "but it decreases its virulence significantly."

"It's rare to find something that has such a strong effect that you can hit one protein so specifically, knock out essentially half its activity, and have such a dramatic result," he says. "Not only did we have a mechanistic explanation, but we could connect what we were seeing in animal studies all the way down to what was happening at the atomic level."


'"/>

Source:Rockefeller University


Related biology news :

1. Plague proteome reveals proteins linked to infection
2. Anti-bacterial additive widespread in U.S. waterways
3. A bacterial genome reveals new targets to combat infectious disease
4. Discovery of key proteins shape could lead to improved bacterial pneumonia vaccine
5. Scientists discover that host cell lipids facilitate bacterial movement
6. Family trees of ancient bacteria reveal evolutionary moves
7. Drug-resistant bacteria on poultry products differ by brand
8. Programmable cells: Engineer turns bacteria into living computers
9. NASA links nanobacteria to kidney stones and other diseases
10. Substance protects resilient staph bacteria
11. Physiological effects of reduced gravity on bacteria
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2017)... ITHACA, N.Y. , June 23, 2017  IBM ... in dairy research, today announced a new collaboration using ... the chances that the global milk supply is impacted ... project, Cornell University has become the newest academic institution ... Chain, a food safety initiative that includes IBM Research, ...
(Date:5/23/2017)... May 23, 2017  Hunova, the first robotic gym for the rehabilitation ... officially launched in Genoa, Italy . The first 30 ... and the USA . The technology was developed and ... by the IIT spin-off Movendo Technology thanks to a 10 million euro ... Release, please click: ...
(Date:5/16/2017)... , May 16, 2017   Bridge ... health organizations, and MD EMR Systems , ... development partner for GE, have established a partnership ... Portal product and the GE Centricity™ products, including ... EMR. These new integrations will ...
Breaking Biology News(10 mins):
(Date:10/11/2017)...  VMS BioMarketing, a leading provider of patient support solutions, ... Educator (CNE) network, which will launch this week. The VMS ... care professionals to enhance the patient care experience by delivering ... health care professionals to help women who have been diagnosed ... ...
(Date:10/10/2017)... ... 10, 2017 , ... San Diego-based team building and cooking events company, Lajollacooks4u, ... The bold new look is part of a transformation to increase awareness, appeal ... growth period. , It will also expand its service offering from its signature gourmet ...
(Date:10/10/2017)... , Oct. 10, 2017 SomaGenics announced the ... NIH to develop RealSeq®-SC (Single Cell), expected to be ... small RNAs (including microRNAs) from single cells using NGS ... the need to accelerate development of approaches to analyze ... "New techniques for measuring levels of mRNAs ...
(Date:10/9/2017)... N.C. (PRWEB) , ... October 09, 2017 , ... At ... announced Dr. Christopher Stubbs, a professor in Harvard University’s Departments of Physics and Astronomy, ... Stubbs was a member of the winning team for the 2015 Breakthrough Prize in ...
Breaking Biology Technology: