Navigation Links
A novel method to measure circadian cycles

Everyone knows morning people and late-night owls. The variation in individual circadian rhythms is an anecdotal as well as experimentally verified fact. But, until now, to systematically study circadian differences (and thereby hope to rout out the underlying genetic causes), scientists have had to rely on prolonged behavioural observation. To screen for and identify circadian rhythm variations in humans, the required period of lengthy observation is prohibitively costly and labor intensive.

Circumventing these technical limitations, Ueli Schibler and colleagues report in the open-access journal PLoS Biology a new method to measure circadian cycles in mammalian cells cultured from tissues other than the suprachiasmatic nucleus. The researchers took skin samples from humans and infected subsequently made tissue cultures with a virus engineered to report with a fluorescent signal when a certain host circadian rhythm gene was expressed. They found that their data jibed with the previously accepted length of the human circadian cycle: 24.5 hours. Because of the sensitivity of their method, Schibler and colleagues also confirmed that, for both humans and mice, circadian rhythms vary substantially between individuals. This suggests that the genetics of the circadian clock likewise varies between individuals.

Unlike an individual's true rhythm, a fibroblast cell culture's rhythm does not vary with changes in light exposure or sleep habits. The researchers point out that their method can expose differences in circadian rhythms; it does not, however, directly measure the signal from the central coordinator of circadian rhythms in the brain, the suprachiasmatic nucleus.

In the future, scientists may use the new method to screen large populations for genetically linked sleep disturbances such as advanced and delayed sleep phase syndromes. They may also use this test to hone in on the genetic mechanism responsible for such conditions. Outsi de the realm of medicine, future genetic studies of circadian rhythm may exploit the method developed by Schibler and colleagues to explore questions about the exact relationship between the suprachiasmatic nucleus and the circadian rhythms of cultured fibroblast cells. Just how does this brain structure coordinate genetic imperatives with environmental input including light fluctuations? And frequent fliers, bleary-eyed in foreign time zones, may get an answer to why waking up is hard to do.


'"/>

Source:Public Library of Science


Related biology news :

1. ASU researchers finds novel chemistry at work to provide parrots vibrant red colors
2. Insight into natural cholesterol control suggests novel cholesterol-lowering therapy
3. GeneNotes - A novel information management software for biologists
4. Endocannabinoids ?the brains cannabis ?demonstrate novel modes of action to stress
5. A novel virus for croup
6. Scientists show that tick-borne flaviviruses use a novel mechanism to evade host defenses
7. UCSD discovery may provide novel method to generate medically useful proteins
8. Updated data on novel HPV vaccine confirms efficacy in large population
9. Neurons generated in the adult brain learn to respond to novel stimuli
10. Federal grant funds research on novel HIV therapy
11. Mutations in the BRAF gene predict sensitivity to a novel class of cancer drugs
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/15/2016)... , June 15, 2016 ... report titled "Gesture Recognition Market by Application Market - Global Industry ... - 2024". According to the report, the  global gesture ... in 2015 and is estimated to grow at ... billion by 2024.  Increasing application of ...
(Date:6/9/2016)... , June 9, 2016 ... Police deploy Teleste,s video security solution to ensure the safety ... France during the major tournament Teleste, ... communications systems and services, announced today that its video security ... to back up public safety across the country. ...
(Date:6/3/2016)... LONDON , June 3, 2016 /PRNewswire/ ... Transport Management) von Nepal ... ,Angebot und Lieferung hochsicherer geprägter Kennzeichen, einschließlich ... weltweit führend in der Produktion und Implementierung ... an der Ausschreibung im Januar teilgenommen, aber ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... SILVER SPRING, Md. , June 23, 2016 ... evidence collected from the crime scene to track the criminal ... sick, and the U.S. Food and Drug Administration (FDA) uses ... Sound far-fetched? It,s not. ... whole genome sequencing to support investigations of foodborne illnesses. Put ...
(Date:6/23/2016)... , June 23, 2016   EpiBiome , a ... $1 million in debt financing from Silicon Valley Bank ... automation and to advance its drug development efforts, as ... facility. "SVB has been an incredible strategic ... services a traditional bank would provide," said Dr. ...
(Date:6/23/2016)... BEACH, Calif. , June 23, 2016  Blueprint ... new biological discoveries to the medical community, has closed ... co-founder Matthew Nunez . "We have ... us with the capital we need to meet our ... will essentially provide us the runway to complete validation ...
(Date:6/23/2016)... ... June 23, 2016 , ... Velocity ... intelligent tools designed, tuned and optimized exclusively for Okuma CNC machining centers at ... result of a collaboration among several companies with expertise in toolholding, cutting tools, ...
Breaking Biology Technology: