Navigation Links
A fisheye view of the deadliest breast cancer

Inflammatory breast cancer (IBC) is the deadliest form of the disease, with fewer than half of those diagnosed today having a five-year prognosis for survival. To find out what drives this most aggressive of human breast cancers, and to rapidly screen for drugs that might stop IBC, researchers at the University of California, San Diego (UCSD), have come up with an unlikely yet extremely promising ally: a transparent fish suitable for xenografts.

More properly, it’s a transgenic zebrafish, a tropical minnow native to streams around the Bay of Bengal and common to aquariums around the world, which was chemically treated to be immune suppressed. In recent years, the zebrafish has vaulted to the top as a laboratory model organism. The zebrafish is tough, easy to breed, and a vertebrate, just like us. Being immune suppressed, the transgenic fish allowed Konstantin Stoletov and colleagues at UCSD to insert a xenograft of human MDA breast cancer cells. Being transparent, the zebrafish’s tissues gave them a window on live human cancer cells in action.

The researchers were particularly interested in the small GTPase RhoC that is overexpressed in highly metastatic forms of breast cancer. Using various fluorescent tags, they labeled human MDA breast cancer cells in two ways to mark the parental MDA cells and to mark tumor cells that overexpress RhoC. They injected both tumor cell lines into the immune-suppressed fish and watched the cancer’s progress over several weeks. The tumor cells homed in on blood vessels, forming tumor-like aggregates and tapping into the fish’s circulatory system by inducing an angiogenic response.

A separate fluorescent labeling allowed the researchers to simultaneously monitor the parental MDA cancer cells and the RhoC-overexpressing tumor cells in the context of the fish vasculature using high-resolution, multicolor confocal microscopy. While the parental MDA cells formed tightly packed aggregates, RhoC-overe xpressing cells scattered within the fish tissue. RhoC overexpression also increased tumor cell membrane dynamics leading to continuous shedding of small cellular fragments.

The transparent fish also let the researchers make high-resolution 3D images of two potential anticancer compounds at work: a vascular endothelial growth factor (VEGF) receptor inhibitor called SU5416, and a protein kinase inhibitor called PP1. Analysis of compound and mock-treated animals revealed that SU5416 leads to shrinkage of tumor cells and tumor-induced vasculature, while PP1 mainly affects tumor cell survival.

In all, the researchers were delighted with their breast cancer model’s ability to work as a real-time study window into IBC tumor behavior and as a rapid screening system for anticancer drugs. In the race against aggressive breast cancers, Stoletov and colleagues see their xenografted, genetically engineered zebrafish as a potential front runner.


'"/>

Source:American Society for Cell Biology


Related biology news :

1. Researchers report breakthrough against world’s deadliest viruses
2. Scientists develop a way to make the deadliest toxin known even more toxic
3. Viral DNA sequence a possible trigger for breast cancer
4. Used in a new way, RNA interference permanently silences key breast cancer gene
5. Biomarkers isolated from saliva successfully predict oral and breast cancer
6. Study reveals dramatic difference between breast cancers in US and Africa
7. Compounds in plastic packaging act as environmental estrogens altering breast genes
8. Alcohol consumption disrupts breastfeeding hormones
9. VCU Massey Cancer Center study shows enzyme linked to spread of breast cancer cells
10. Scientists discover genetic pathway responsible for breast cancer cell growth
11. Study hints at role of stem cell genes in testicular, breast cancers
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/30/2017)... , March 30, 2017  On April 6-7, ... Hack the Genome hackathon at Microsoft,s headquarters ... two-day competition will focus on developing health and wellness ... Hack the Genome is the first ... tremendous. The world,s largest companies in the genomics, tech ...
(Date:3/29/2017)... , March 29, 2017  higi, the health IT ... North America , today announced a ... the acquisition of EveryMove. The new investment and acquisition ... of tools to transform population health activities through the ... data. higi collects and secures data today ...
(Date:3/24/2017)... -- The Controller General of Immigration from Maldives Mr. ... have received the prestigious international IAIR Award for the most innovative high ... ... Maldives Immigration Controller ... (small picture on the right) have received the IAIR award for the ...
Breaking Biology News(10 mins):
(Date:9/19/2017)... ... September 19, 2017 , ... The ... cybersecurity regulations have transitioned into full force and effect. The law requires ... (“Covered Entities”) to conduct an annual, professional, comprehensive cybersecurity risk assessment, with ...
(Date:9/18/2017)... ... September 18, 2017 , ... ... produce biochar, briquettes, and torrefied wood is the topic of a September ... characterize the potential economic viability of transportable biomass conversion facilities for producing biochar, ...
(Date:9/18/2017)... a growing leader in Electronic Trial Master file solutions , ... organization, Multidisciplinary Association for Psychedelic Studies (MAPS). ... MAPS Public Benefit Corporation selects eTMF ... ... the treatment of Posttraumatic Stress Disorder (PTSD). MAPS also reached agreement ...
(Date:9/17/2017)... , ... September 17, 2017 , ... ... the South Korean Ministry of Food and Drug Safety (KMFDS) for an Investigational ... against the Middle East Respiratory Syndrome coronavirus (MERS-CoV). The study in Korea represents ...
Breaking Biology Technology: