Navigation Links
A fisheye view of the deadliest breast cancer

Inflammatory breast cancer (IBC) is the deadliest form of the disease, with fewer than half of those diagnosed today having a five-year prognosis for survival. To find out what drives this most aggressive of human breast cancers, and to rapidly screen for drugs that might stop IBC, researchers at the University of California, San Diego (UCSD), have come up with an unlikely yet extremely promising ally: a transparent fish suitable for xenografts.

More properly, it’s a transgenic zebrafish, a tropical minnow native to streams around the Bay of Bengal and common to aquariums around the world, which was chemically treated to be immune suppressed. In recent years, the zebrafish has vaulted to the top as a laboratory model organism. The zebrafish is tough, easy to breed, and a vertebrate, just like us. Being immune suppressed, the transgenic fish allowed Konstantin Stoletov and colleagues at UCSD to insert a xenograft of human MDA breast cancer cells. Being transparent, the zebrafish’s tissues gave them a window on live human cancer cells in action.

The researchers were particularly interested in the small GTPase RhoC that is overexpressed in highly metastatic forms of breast cancer. Using various fluorescent tags, they labeled human MDA breast cancer cells in two ways to mark the parental MDA cells and to mark tumor cells that overexpress RhoC. They injected both tumor cell lines into the immune-suppressed fish and watched the cancer’s progress over several weeks. The tumor cells homed in on blood vessels, forming tumor-like aggregates and tapping into the fish’s circulatory system by inducing an angiogenic response.

A separate fluorescent labeling allowed the researchers to simultaneously monitor the parental MDA cancer cells and the RhoC-overexpressing tumor cells in the context of the fish vasculature using high-resolution, multicolor confocal microscopy. While the parental MDA cells formed tightly packed aggregates, RhoC-overe xpressing cells scattered within the fish tissue. RhoC overexpression also increased tumor cell membrane dynamics leading to continuous shedding of small cellular fragments.

The transparent fish also let the researchers make high-resolution 3D images of two potential anticancer compounds at work: a vascular endothelial growth factor (VEGF) receptor inhibitor called SU5416, and a protein kinase inhibitor called PP1. Analysis of compound and mock-treated animals revealed that SU5416 leads to shrinkage of tumor cells and tumor-induced vasculature, while PP1 mainly affects tumor cell survival.

In all, the researchers were delighted with their breast cancer model’s ability to work as a real-time study window into IBC tumor behavior and as a rapid screening system for anticancer drugs. In the race against aggressive breast cancers, Stoletov and colleagues see their xenografted, genetically engineered zebrafish as a potential front runner.


'"/>

Source:American Society for Cell Biology


Related biology news :

1. Researchers report breakthrough against world’s deadliest viruses
2. Scientists develop a way to make the deadliest toxin known even more toxic
3. Viral DNA sequence a possible trigger for breast cancer
4. Used in a new way, RNA interference permanently silences key breast cancer gene
5. Biomarkers isolated from saliva successfully predict oral and breast cancer
6. Study reveals dramatic difference between breast cancers in US and Africa
7. Compounds in plastic packaging act as environmental estrogens altering breast genes
8. Alcohol consumption disrupts breastfeeding hormones
9. VCU Massey Cancer Center study shows enzyme linked to spread of breast cancer cells
10. Scientists discover genetic pathway responsible for breast cancer cell growth
11. Study hints at role of stem cell genes in testicular, breast cancers
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/15/2016)... 2016  There is much more to innovative access ... engine. Continental will demonstrate the intelligence of today,s solutions ... . Through the combination of the keyless entry and ... elements, the international technology company is opening up new ... "The integration of biometric elements brings our ...
(Date:12/12/2016)...  Researchers at Trinity College, Dublin, are opening ... the material with Silly Putty. The mixture (known as ... to sense pulse, blood pressure, respiration, and even ... The research team,s findings were published Thursday ... http://science.sciencemag.org/content/354/6317/1257 ...
(Date:12/8/2016)... Calif. , Dec. 8, 2016  Singulex, Inc., ... Molecule Counting technology, entered into a license and supply ... serving science. The agreement provides Singulex access to Thermo ... Europe is used to diagnose systemic ... United States to aid in assessing the ...
Breaking Biology News(10 mins):
(Date:1/23/2017)... England , January 23, 2017 ... today announces completion of its Series D financing, raising $35 ... one new investor, Wondfo Biotech. ... Development of the Atlas Genetics io® ... the Chlamydia trachomatis (CT) test announced in February 2016.  This ...
(Date:1/21/2017)... ... January 20, 2017 , ... G&L ... the healthcare industry ( http://www.gandlscientific.com ), has announced the opening of new offices ... and scientific consultants and contractors. This is the latest step in G&L’s expansion ...
(Date:1/21/2017)... , Jan. 20, 2017 Aratana Therapeutics, ... company focused on the licensing, development and commercialization of ... Pharm,s Best Company in North America ... the award based on the FDA approval ... (grapiprant tablets), ENTYCE ® (capromorelin oral solution) and ...
(Date:1/20/2017)... January 20, 2017 http://www.Financialbuzz.com ... one of leading causes of death worldwide. There were ... number of cancer related deaths increased gradually over time, ... incidence rate of various cancers continues to drive demand ... report by Global Market Insights, Inc. cancer biological therapy ...
Breaking Biology Technology: