Navigation Links
A biosensor layered like lasagna

In a mixing of pasta metaphors, Pacific Northwest National Laboratory scientists have used electrostatic attraction to layer reactive biological molecules lasagna-like around spaghetti-like carbon nanotubes.

This configuration can accommodate a wide range of applications, from ultra-precise blood-sugar monitoring to infectious-agent detection, said Yuehe Lin, who led the research at the Department of Energy campus' W.R. Wiley Environmental Molecular Sciences Laboratory.

The technique, described in the current (April) Journal of Nanoscience and Nanotechnology, enables enzymes, with the help of a long, noodle-like polymer molecule, to self-assemble layer-by-layer on a single carbon nanotube.

Lin and co-author Guodong Liu, a postdoctoral fellow in Lin's group, coaxed electrostatic clinginess in a polymer and an oppositely charged protein-enzyme, in this case glucose oxidase, which reacts in the presence of blood sugar. The catalyzed products from the reaction ping the carbon nanotube; if the tube is connected to an electrode, the tube will carry a signal that corresponds precisely with the amount of glucose detected. The first polymer binds to the carbon nanotube. Enzymes are attracted to the polymer, leaving an outer layer for the next polymer of opposite charge to cling to, and so on.

An individual strand coated with one to six layers ranges from 30 nanometers to about 50 nanometers thick. "Each polymer layer is porous," Lin said. "This allows the glucose to diffuse in and come into contact with the enzymes."

"The polymers trap the enzymes in place," Liu said. "You can go up to five or six layers to improve the sensitivity of the detector, but after that, the more enzyme layers" inhibit diffusion of the glucose.

Now that the glucose enzyme biosensor has passed the test, Lin said, it should be possible to build a similar sensor using other enzymes that react specifically with other biological chemicals, environmen tal agents or even microbes and their toxic byproducts. Lin and colleagues also reported, in February issue of Analytical Chemistry, having used a similar technique to construct sensors for nerve agents.


'"/>

Source:DOE/Pacific Northwest National Laboratory


Related biology news :

1. Rochester scientists develop fast-working biosensor
2. Smell of success for nanobiosensors
3. A new portable biosensor detects traces of contaminants in food more quickly and cheaply
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/16/2016)... 16, 2016 The global ... to reach USD 1.83 billion by 2024, according ... Inc. Technological proliferation and increasing demand in commercial ... to drive the market growth.      ... The development of advanced multimodal techniques for biometric ...
(Date:6/7/2016)... TORONTO , June 7, 2016  Syngrafii ... begun a business relationship that includes integrating Syngrafii,s ... pilot branch project. This collaboration will result in ... for the credit union, while maintaining existing document ... http://photos.prnewswire.com/prnh/20160606/375871LOGO ...
(Date:6/2/2016)... 2016 Perimeter Surveillance & Detection ... Physical Infrastructure, Support & Other Service  The ... offers comprehensive analysis of the global Border Security ... revenues of $17.98 billion in 2016. Now: ... leader in software and hardware technologies for advanced video ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... discussions on a range of subjects including policies, debt and ... Poloz. Speaking at a lecture to the Canadian ... to the country,s inflation target, which is set by both ... "In certain areas there needs to be ... why not sit down and address strategy together?" ...
(Date:6/23/2016)... , ... June 23, 2016 , ... ... pleased to announce the launch of their brand, UP4™ Probiotics, into Target stores ... 35 years, is proud to add Target to its list of well-respected retailers. ...
(Date:6/23/2016)... ... June 23, 2016 , ... Charm Sciences, Inc. ... test has received AOAC Research Institute approval 061601. , “This is another AOAC-RI ... stated Bob Salter, Vice President of Regulatory and Industrial Affairs. “The Peel Plate ...
(Date:6/23/2016)... ... ... STACS DNA Inc., the sample tracking software company, today announced that Dr. ... STACS DNA as a Field Application Specialist. , “I am thrilled that Dr. ... STACS DNA. “In further expanding our capacity as a scientific integrator, Hays brings a ...
Breaking Biology Technology: