Navigation Links
Zinc finger nuclease, immunoprecipitation methods featured in Cold Spring Harbor Protocols
Date:8/2/2010

COLD SPRING HARBOR, N.Y. (Mon., Aug. 2, 2010) -- Zinc finger nucleases (ZFNs) are artificial restriction enzymes made by fusing an engineered zinc finger DNA-binding domain to the DNA cleavage domain of a restriction enzyme. ZFNs can be used to generate targeted genomic deletions of large segments of DNA in a wide variety of cell types and organisms. In the August issue of the journal Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/TOCs/toc8_10.dtl), Jin-Soo Kim and colleagues (http://chem.snu.ac.kr/eng/Faculty/faculty_detail.asp?seqno=1015&link=faculty) present "Analysis of Targeted Chromosomal Deletions Induced by Zinc Finger Nucleases," a detailed protocol for the detection and analysis of large genomic deletions in cultured cells introduced by the expression of ZFNs. The method described allows researchers to detect and estimate the frequency of ZFN-induced genomic deletions by simple PCR-based methods. This featured protocol is freely available on the journal's website (http://cshprotocols.cshlp.org/cgi/content/full/2010/8/pdb.prot5477).

Immunoprecipitation is a commonly used technique for isolating and purifying a protein of interest. An antibody for the protein is incubated with a cell extract, and the resulting antibody/antigen complex is pulled out of solution. The method used for preparation of the cell extract can be critical for the experiment's success. The choice of lysis conditions must be tailored to the nature of the epitope recognized by the immunoprecipitating antibody. "Lysis of Cultured Cells for Immunoprecipitation," featured in the August issue of Cold Spring Harbor Protocols (http://cshprotocols.cshlp.o
'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Page: 1 2

Related biology news :

1. ID Solutions Achieves Full-Production Delivery and Payment for Fingerprint Processing Software for TWIC
2. Kount Receives Patent for Device Fingerprinting
3. Fingerprinting method tracks mercury emissions from coal
4. Genes that control cell death fingered in age-related hearing loss
5. Frost & Sullivan Recognizes Precise Biometrics as a Key Player in the Non-AFIS Fingerprint Market
6. identiMetrics & BIO-key(R) Provide Biometric Finger Scanning for School Food Service Nationwide
7. DigitalPersona Places Identity Protection at Peoples Fingertips
8. Futronic Launches FS22 Fingerprint Access Control Device
9. InvoTech Selects M2SYS Technology for Leading-Edge Fingerprint Software Solution
10. MIT researchers explain mystery of gravity fingers
11. BIO-key(R) Fingerprint Biometrics Media Spotlights
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/18/2014)... found clownfish larvae can swim up to 400 kilometres in ... cope with environmental change. , Clownfish spend their entire adult ... babies they must wander the open ocean, says study co-author, ... Coral Reef Studies (Coral CoE) at James Cook University. , ... now we,ve been given a rare glimpse into how far ...
(Date:9/17/2014)... UCLA atmospheric scientists were instrumental in the creation of ... tool to classify the fire threat potential of the ... a spark into an inferno. The index was introduced ... with UCLA and San Diego Gas and Electric. , ... to "extreme" that will be used to help fire ...
(Date:9/17/2014)... EC-FP5 four-year project, delivering its first release in 2004. ... in several EC projects, as a part of the ... BON), to increase the general awareness of the work ... dissemination of the Fauna Europaea results, the ... e-Publishing tools to prepare data papers for all 56 ...
Breaking Biology News(10 mins):Nemo's epic journey to find a new home 2UCLA scientists play key role in developing new Santa Ana Wildfire Threat Index 2Contributions on Fauna Europaea: Data papers as innovative model on expert involvement 2
... in obese laboratory rats may provide a less-invasive alternative ... Endocrinology . Scientists at Cincinnati Children,s Hospital ... of bile from the bile duct into the small ... bariatric surgeries such as gastric by-pass. They named the ...
... effect of different dosages of vitamin D supplementation in ... within a range recommended by some pediatric societies. However, ... lower range recommended by the Institute of Medicine, according ... JAMA , a theme issue on child health. ...
... From methanol to formaldehyde - this reaction is the ... Using catalysts made of gold particles, formaldehyde could be ... methods. Just how the mysterious gold catalyst works has ... the Ruhr-Universitt Bochum in a cooperation project. In the ...
Cached Biology News:Study finds possible alternative to bariatric weight loss surgery 2Optimal vitamin D dosage for infants uncertain 2Optimal vitamin D dosage for infants uncertain 3Mysterious catalyst explained 2
(Date:9/18/2014)... , Sept. 18, 2014 About ... testing, is performed outside a clinical laboratory. It ... in administrating appropriate treatments and medication. POCT is ... prevalence of lifestyle diseases such as diabetes, heart ... user-friendly. POCT devices are standardized to minimize errors ...
(Date:9/18/2014)... , September 18, 2014 /PRNewswire/ ... Markets ( http://www.researchandmarkets.com/research/czvg3s/biocompatible ) has announced ... Materials - Global Strategic Business Report" ... (Logo: http://photos.prnewswire.com/prnh/20130307/600769) ,This report analyzes the ... US$ Million by the following Product ...
(Date:9/18/2014)... DUBLIN , Sept. 18, 2014 ... addition of the "Global N-acetylcysteine Industry Report ... The Global N-acetylcysteine Industry Report 2014 is ... state of the global N-acetylcysteine industry. ... industry including definitions, classifications, applications and industry chain ...
(Date:9/18/2014)... 2014  U.S. biotech company Kultevat has ... for technology developed by the Donald Danforth Plant ... including for applications in production of natural rubber in ... was developed under the direction of Roger Beachy ... president of the Danforth Plant Science Center, and is ...
Breaking Biology Technology:POCT Market in China 2014-2018 2Biocompatible Materials - Global Strategic Business Report 2014 2Biocompatible Materials - Global Strategic Business Report 2014 3Global N-acetylcysteine Industry Report 2014 2Kultevat obtains license of gene switch technology 2
... (NPWH) Encourages Women to Get Informed and Ask ... growing awareness of HPV,(human papillomavirus), the primary cause of ... of Nurse Practitioners in Women,s,Health (NPWH) found that many ... prevent the disease. Despite the fact that there is ...
... Inc.,(Nasdaq: OSTE ), a leader in the emerging ... total revenue for the year,ending December 31, 2007 increased ... year ended December 31, 2006. Revenue from "core" product,lines, ... year,2007, an increase of 15% over core product revenue ...
... still flawed government-wide plan for nanotechnology risk research ... Federally-funded studies essential to managing possible risks from ... top-down strategy tied to projected commercialization, expected human ... , The National Nanotechnology Initiatives (NNI) ...
Cached Biology Technology:Survey Shows Most Women Can't Separate Fact From Fiction When It Comes to Cervical Cancer Prevention 2Survey Shows Most Women Can't Separate Fact From Fiction When It Comes to Cervical Cancer Prevention 3Survey Shows Most Women Can't Separate Fact From Fiction When It Comes to Cervical Cancer Prevention 4Osteotech Reports 2007 Full Year and Fourth Quarter Financial Results; Full Year Revenue of $104.3 Million Exceeds $100 Million Milestone; Diluted Earnings Per Share Was $.15 in 2007 2Osteotech Reports 2007 Full Year and Fourth Quarter Financial Results; Full Year Revenue of $104.3 Million Exceeds $100 Million Milestone; Diluted Earnings Per Share Was $.15 in 2007 3Osteotech Reports 2007 Full Year and Fourth Quarter Financial Results; Full Year Revenue of $104.3 Million Exceeds $100 Million Milestone; Diluted Earnings Per Share Was $.15 in 2007 4Osteotech Reports 2007 Full Year and Fourth Quarter Financial Results; Full Year Revenue of $104.3 Million Exceeds $100 Million Milestone; Diluted Earnings Per Share Was $.15 in 2007 5Osteotech Reports 2007 Full Year and Fourth Quarter Financial Results; Full Year Revenue of $104.3 Million Exceeds $100 Million Milestone; Diluted Earnings Per Share Was $.15 in 2007 6Federal nanotech risk research plan still comes up short 2
... functional protein microarray is the first microarray ... interactions on a proteome scale. The Yeast ... frames (ORFs) expressed as 5'-GST fusions, purified ... inch x 3 inch nitrocellulose-coated slide. With ...
... Useful for the mechanical harvesting of cells ... ensures even contact with the growth surface ... 75cm flasks • Individually wrapped • Sterilized ... This CLS number is a new product ...
... description: Designed for use with dishes and ... is a new product number, created to ... no availability yet, please order under the ... service for assistance. Comp Dim: blade L ...
... combine the proven features of the pET, ... provides all of the benefits and versatility ... backbone. The pTriEx vectors have been extensively ... in all three expression systems. In each ...
Biology Products: