Navigation Links
With chemical modification, stable RNA nanoparticles go 3-D
Date:1/20/2011

CINCINNATIFor years, RNA has seemed an elusive tool in nanotechnology researcheasily manipulated into a variety of structures, yet susceptible to quick destruction when confronted with a commonly found enzyme.

"The enzyme RNase cuts RNA randomly into small pieces, very efficiently and within minutes," explains Peixuan Guo, PhD, Dane and Mary Louise Miller Endowed Chair and professor of biomedical engineering at the University of Cincinnati (UC). "Moreover, RNase is present everywhere, making the preparation of RNA in a lab extremely difficult."

But by replacing a chemical group in the macromolecule, Guo says he and fellow researchers have found a way to bypass RNase and create stable three-dimensional configurations of RNA, greatly expanding the possibilities for RNA in nanotechnology (the engineering of functional systems at the molecular scale).

Their results, "Fabrication of Stable and RNase-Resistant RNA Nanoparticles Active in Gearing the Nanomotors for Viral DNA Packaging," are published online at journal ACS Nano.

In their work, Guo and his colleagues focused on the ribose rings that, together with alternating phosphate groups, form the backbone of RNA. By changing one section of the ribose ring, Guo and his team altered the structure of the molecule, making it unable to bind with RNase and able to resist degradation.

"RNase interaction with RNA requires a match of structural conformation," says Guo. "When RNA conformation has changed, the RNase cannot recognize RNA and the binding becomes an issue."

While he says previous researchers have shown this alteration makes RNA stable in a double helix, they did not study its potential to affect the folding of RNA into a three-dimensional structure necessary for nanotechnology.

After creating the RNA nanoparticle, Guo and his colleagues successfully used it to power the DNA packaging nanomotor of bacteriophage phi29, a virus that infects bacteria.

"We found that the modified RNA can fold into its 3-D structure appropriately, and can carry out its biological functions after modification," says Guo. "Our results demonstrate that it is practical to produce RNase-resistant, biologically active, and stable RNA for application in nanotechnology."

Because stable RNA molecules can be used to assemble a variety of nanostructures, Guo says they are an ideal tool to deliver targeted therapies to cancerous or viral-infected cells:

"RNA nanoparticles can be fabricated with a level of simplicity characteristic of DNA while possessing versatile structure and catalytic function similar to that of proteins. With this RNA modification, hopefully we can open new avenues of study in RNA nanotechnology."


'/>"/>

Contact: Katy Cosse
kathryn.cosse@uc.edu
513-558-0207
University of Cincinnati Academic Health Center
Source:Eurekalert

Related biology news :

1. Chemical equator discovery will aid pollution mapping
2. Researcher working on destruction of chemical weapons
3. Argonne scientists peer into heart of compound that may detect chemical, biological weapons
4. American Chemical Societys Weekly PressPac -- Sept. 24, 2008
5. A major prize in the chemical sciences announced by the Camille and Henry Dreyfus Foundation
6. American Chemical Societys Weekly PressPac
7. American Chemical Society Weekly PressPac Oct. 15, 2008
8. UC Davis chemical ecologist wins major award
9. Sniffing out a better chemical sensor
10. Simple chemical procedure augments therapeutic potential of stem cells
11. Researchers use chemical from medicinal plants to fight HIV
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/2/2017)... Australian stem cell and regenerative medicine company, ... an agreement with the Monash Lung Biology Network, a ... and Department of Pharmacology at Monash University, ... to support the use of Cymerus™ mesenchymal stem cells ... Asthma is a chronic, long term lung condition recognised ...
(Date:2/28/2017)... BARCELONA , Spanien, 27. Februar 2017 /PRNewswire/ ... durch Iris-Scan, wird seine erstklassige biometrische Lösung ... Snapdragon™ 835 mit X16 LTE auf dem ... 2. März) am Qualcomm-Stand in Halle 3, ... 835-Prozessor beinhaltet die Sicherheitsplattform Qualcomm Haven™ – ...
(Date:2/22/2017)... , Feb. 22, 2017 With ... 2021, ABI Research identifies four technologies that innovative ... to secure significant share in the changing competitive ... and passive authentication.   "Companies can ... comes to security," says Dimitrios Pavlakis , ...
Breaking Biology News(10 mins):
(Date:3/28/2017)... (PRWEB) , ... March 28, 2017 , ... ... at a Marketing Roundtable on March 22 in Philadelphia. The event was offered ... , The Marketing Roundtable featured breakout groups and interaction with speakers ...
(Date:3/28/2017)... WASHINGTON , March 28, 2017  (AACR17, ... single-cell sequencing during the American Association for Cancer Research ... Center in Washington, D.C. , April ... gene expression of thousands of cells at the individual ... Experts on-hand at AACR to discuss expanded ...
(Date:3/28/2017)... Dr. Chris Yu , Chief ... Anpac Bio-Medical Science Company , announced this week ... and reporting over 40,000 cases worldwide of the ... liquid biopsy tests. Described as "game ... Anpac Bio,s CDA medical devices and technology effectively ...
(Date:3/28/2017)... , March 28, 2017 ... and unpredictable sector due to the scientifically intensive ... include medical, agricultural, environmental, and industrial. In today,s pre-market ... Portola Pharmaceuticals Inc. (NASDAQ: PTLA), OvaScience Inc. (NASDAQ: ... Therapeutix Inc. (NASDAQ: OCUL ). Learn ...
Breaking Biology Technology: