Navigation Links
With chemical modification, stable RNA nanoparticles go 3-D
Date:1/20/2011

CINCINNATIFor years, RNA has seemed an elusive tool in nanotechnology researcheasily manipulated into a variety of structures, yet susceptible to quick destruction when confronted with a commonly found enzyme.

"The enzyme RNase cuts RNA randomly into small pieces, very efficiently and within minutes," explains Peixuan Guo, PhD, Dane and Mary Louise Miller Endowed Chair and professor of biomedical engineering at the University of Cincinnati (UC). "Moreover, RNase is present everywhere, making the preparation of RNA in a lab extremely difficult."

But by replacing a chemical group in the macromolecule, Guo says he and fellow researchers have found a way to bypass RNase and create stable three-dimensional configurations of RNA, greatly expanding the possibilities for RNA in nanotechnology (the engineering of functional systems at the molecular scale).

Their results, "Fabrication of Stable and RNase-Resistant RNA Nanoparticles Active in Gearing the Nanomotors for Viral DNA Packaging," are published online at journal ACS Nano.

In their work, Guo and his colleagues focused on the ribose rings that, together with alternating phosphate groups, form the backbone of RNA. By changing one section of the ribose ring, Guo and his team altered the structure of the molecule, making it unable to bind with RNase and able to resist degradation.

"RNase interaction with RNA requires a match of structural conformation," says Guo. "When RNA conformation has changed, the RNase cannot recognize RNA and the binding becomes an issue."

While he says previous researchers have shown this alteration makes RNA stable in a double helix, they did not study its potential to affect the folding of RNA into a three-dimensional structure necessary for nanotechnology.

After creating the RNA nanoparticle, Guo and his colleagues successfully used it to power the DNA packaging nanomotor of bacteriophage phi29, a virus that infects bacteria.

"We found that the modified RNA can fold into its 3-D structure appropriately, and can carry out its biological functions after modification," says Guo. "Our results demonstrate that it is practical to produce RNase-resistant, biologically active, and stable RNA for application in nanotechnology."

Because stable RNA molecules can be used to assemble a variety of nanostructures, Guo says they are an ideal tool to deliver targeted therapies to cancerous or viral-infected cells:

"RNA nanoparticles can be fabricated with a level of simplicity characteristic of DNA while possessing versatile structure and catalytic function similar to that of proteins. With this RNA modification, hopefully we can open new avenues of study in RNA nanotechnology."


'/>"/>

Contact: Katy Cosse
kathryn.cosse@uc.edu
513-558-0207
University of Cincinnati Academic Health Center
Source:Eurekalert

Related biology news :

1. Chemical equator discovery will aid pollution mapping
2. Researcher working on destruction of chemical weapons
3. Argonne scientists peer into heart of compound that may detect chemical, biological weapons
4. American Chemical Societys Weekly PressPac -- Sept. 24, 2008
5. A major prize in the chemical sciences announced by the Camille and Henry Dreyfus Foundation
6. American Chemical Societys Weekly PressPac
7. American Chemical Society Weekly PressPac Oct. 15, 2008
8. UC Davis chemical ecologist wins major award
9. Sniffing out a better chemical sensor
10. Simple chemical procedure augments therapeutic potential of stem cells
11. Researchers use chemical from medicinal plants to fight HIV
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/5/2017)... 2017 Today HYPR Corp. , leading ... component of the HYPR platform is officially FIDO® ... security architecture that empowers biometric authentication across Fortune 500 ... secured over 15 million users across the financial services ... home product suites and physical access represent a growing ...
(Date:3/30/2017)... , March 30, 2017 The research team ... for three-dimensional (3D) fingerprint identification by adopting ground breaking 3D fingerprint ... new realm of speed and accuracy for use in identification, crime ... affordable cost. ... A ...
(Date:3/24/2017)... , March 24, 2017 The Controller General of ... Mr. Abdulla Algeen have received the prestigious international IAIR Award ... Continue Reading ... ... and Deputy Controller Abdulla Algeen (small picture on the right) have received ...
Breaking Biology News(10 mins):
(Date:5/26/2017)... Ca (PRWEB) , ... May 25, 2017 , ... ... truly understanding the full process behind each occurrence. Live cell imaging using fluorescence ... this webinar, the use of automated fluorescence microscopy methods will be discussed, from ...
(Date:5/24/2017)... ... May 24, 2017 , ... Today, the South ... Recovery’s Advanced Biological Nutrient Recovery (ABNR™) technology at its 4,000,000 gallon per day ... upgrade to sustainably meet current and future nutrient discharge regulations. The ABNR platform, ...
(Date:5/24/2017)... (PRWEB) , ... May 24, 2017 , ... ... systems are increasingly being developed with Wi-Fi connectivity to reduce the amount of ... room to room. In addition, compact mobile devices including infusion pumps, heart and ...
(Date:5/23/2017)... ... ... A recent survey conducted by the Weed Science Society of America (WSSA) ranks ... of broadleaf crops, fruits and vegetables, while common lambsquarters ranks as the weed most ... the 2016 survey, the second conducted by WSSA. A 2015 baseline survey explored ...
Breaking Biology Technology: