Navigation Links
Wisconsin scientists find genetic recipe to turn stem cells to blood
Date:7/14/2014

MADISON, Wis. The ability to reliably and safely make in the laboratory all of the different types of cells in human blood is one key step closer to reality.

Writing today in the journal Nature Communications, a group led by University of Wisconsin-Madison stem cell researcher Igor Slukvin reports the discovery of two genetic programs responsible for taking blank-slate stem cells and turning them into both red and the array of white cells that make up human blood.

The research is important because it identifies how nature itself makes blood products at the earliest stages of development. The discovery gives scientists the tools to make the cells themselves, investigate how blood cells develop and produce clinically relevant blood products.

"This is the first demonstration of the production of different kinds of cells from human pluripotent stem cells using transcription factors," explains Slukvin, referencing the proteins that bind to DNA and control the flow of genetic information, which ultimately determines the developmental fate of undifferentiated stem cells.

During development, blood cells emerge in the aorta, a major blood vessel in the embryo. There, blood cells, including hematopoietic stem cells, are generated by budding from a unique population of what scientists call hemogenic endothelial cells. The new report identifies two distinct groups of transcription factors that can directly convert human stem cells into the hemogenic endothelial cells, which subsequently develop into various types of blood cells.

The factors identified by Slukvin's group were capable of making the range of human blood cells, including white blood cells, red blood cells and megakaryocytes, commonly used blood products.

"By overexpressing just two transcription factors, we can, in the laboratory dish, reproduce the sequence of events we see in the embryo" where blood is made, says Slukvin of the Department of Pathology and Laboratory Medicine in the UW School of Medicine and Public Health and the Wisconsin National Primate Research Center.

The method developed by Slukvin's group was shown to produce blood cells in abundance. For every million stem cells, the researchers were able to produce 30 million blood cells.

A critical aspect of the work is the use of modified messenger RNA to direct stem cells toward particular developmental fates. The new approach makes it possible to induce cells without introducing any genetic artifacts. By co-opting nature's method of making cells and avoiding all potential genetic artifacts, cells for therapy can be made safer.

"You can do it without a virus, and genome integrity is not affected," Slukvin notes. Moreover, while the new work shows that blood can be made by manipulating genetic mechanisms, the approach is likely to be true as well for making other types of cells with therapeutic potential, including cells of the pancreas and heart.

An unfulfilled aspiration, says Slukvin, is to make hematopoietic stem cells, multipotent stem cells found in bone marrow. Hematopoietic stem cells are used to treat some cancers, including leukemia and multiple myeloma. Devising a method for producing them in the lab remains a significant challenge.

"We still don't know how to do that," Slukvin notes, "but our new approach to making blood cells will give us an opportunity to model their development in a dish and identify novel hematopoietic stem cell factors."

The study was conducted under the umbrella of the Progenitor Cell Biology Consortium, run by National Heart, Lung and Blood Institute, part of the National Institutes of Health, and involved a collaboration of scientists at UW-Madison, the Morgridge Institute for Research, the University of Minnesota at the Twin Cities and the Houston Methodist Research Institute.

In addition to Slukvin, authors of the new report include Irina Elcheva, Vera Brok-Volchanskaya, Akhilesh Kumar, Patricia Liu, Jeong-Hee Lee, Lilian Tong and Maxim Vodyanik, all of the Wisconsin National Primate Research Center; Scott Swanson, Ron Stewart and James A. Thomson of the Morgridge Institute for Research; Michael Kyba of the University of Minnesota's Lillehei Heart Institute; and Eduard Yakubov and John Cooke of the Center for Cardiovascular Regeneration of the Houston Methodist Research Institute.
'/>"/>

Contact: Igor Slukvin
islukvin@wisc.edu
608-263-0058
University of Wisconsin-Madison
Source:Eurekalert  

Related biology news :

1. University of Wisconsin chemists find new compounds to curb staph infection
2. Weather, yield compared for horticultural crops in Wisconsin, southern Ontario
3. Stanford scientists develop gene therapy approach to grow blood vessels in ischemic limbs
4. Queens scientists seek vaccine for Pseudomonas infection
5. Scientists produce eye structures from human blood-derived stem cells
6. American Society of Plant Biologists honors early career women scientists
7. Brandeis scientists win prestigious prize for circadian rhythms research
8. Scientists discover new method of proton transfer
9. Salk scientists open new window into how cancers override cellular growth controls
10. WileyChina.com - Now Featuring Bespoke Pages for China’s Life Scientists
11. Scientists win $2 million to study new pathway in development and maintenance of lymphoma
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Wisconsin scientists find genetic recipe to turn stem cells to blood
(Date:4/5/2017)... YORK , April 5, 2017 Today ... is announcing that the server component of the HYPR ... known for providing the end-to-end security architecture that empowers ... HYPR has already secured over 15 million ... makers including manufacturers of connected home product suites and ...
(Date:3/30/2017)... LOS ANGELES , March 30, 2017  On ... Hack the Genome hackathon at ... This exciting two-day competition will focus on developing health ... experience. Hack the Genome is ... has been tremendous. The world,s largest companies in the ...
(Date:3/28/2017)... The report "Video Surveillance Market ... Storage Devices), Software (Video Analytics, VMS), and Service (VSaaS, ... to 2022", published by MarketsandMarkets, the market was valued ... to reach USD 75.64 Billion by 2022, at a ... year considered for the study is 2016 and the ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... 12, 2017 , ... They call it the “hairy ball.” ... depiction of a system of linkages and connections so complex and dense that ... computer science at Worcester Polytechnic Institute (WPI) and director of the university’s bioinformatics ...
(Date:10/12/2017)... ca (PRWEB) , ... October 12, 2017 , ... ... the Surgical Wound Market with the addition of its newest module, US Hemostats ... $1.2B market for thrombin hemostats, absorbable hemostats, fibrin sealants, synthetic sealants and biologic ...
(Date:10/11/2017)... ... October 11, 2017 , ... ... in its endogenous context, enabling overexpression experiments and avoiding the use of exogenous ... RNA guides is transformative for performing systematic gain-of-function studies. , This complement ...
(Date:10/11/2017)... , Oct. 11, 2017  VMS BioMarketing, a leading provider ... nationwide oncology Clinical Nurse Educator (CNE) network, which will launch ... for communication among health care professionals to enhance the patient ... office staff, and other health care professionals to help women ... cancer. ...
Breaking Biology Technology: