Navigation Links
Widely used virus assay shown unreliable when compared to other methods
Date:10/21/2009

Blacksburg, Va. -- In the course of doing research on the mosquito-borne pathogens chikungunya virus (CHIKV) and o' nyong-nyong virus (ONNV), Virginia Tech researchers have discovered an inconvenient truth about an assay, strand-specific quantitative real-time PCR (ssqPCR), increasingly being used to detect and measure replicating viral RNA in infected cells and tissues. The method most labs are using for ssqPCR is unreliable.

The research appears in the Wednesday, October 14, 2009, issue of PLoS ONE, in the article, "Accurate Strand-Specific Quantification of Viral RNA," by Nicole E. Plaskon of Richmond Va., a Master of Science in life sciences candidate in the College of Agriculture and Life Sciences, and entomology Assistant Professors Zach N. Adelman and Kevin M. Myles, all with the Fralin Life Science Institute..

CHIKV has sickened millions of people in India and Africa in the last five years 1.3 million in India alone. ONNV has also previously caused large outbreaks of human disease with cases numbering in the millions. In studying virus infection of the mosquito, the Virginia Tech researchers developed a novel assay that detects and measures anti-genomic copies of the viral genome. This differs from traditional assays that simply measure viral nucleic acids associated with infection, regardless of origin.

"The application of real-time PCR to the detection and quantification of specific strands of viral RNA is becoming an increasingly important tool in the study of RNA viruses. As a result, multiple types of ssqPCR assays have been described and are in widespread use. However, no study has yet determined if the accuracy with which the different types of assays detect and quantify specific strands of viral RNA are equivalent. It turns out they are not, and the most frequently used method is the most error prone," said Myles.

"A less frequently used ssqPCR assay turned out to be more accurate," said Adelman.

"The fact that many labs have been using assays prone to error may have led to some wrong conclusions," Adelman said. "Using the more accurate assays will lead to more accurate conclusions and better science."

Although Myles and Adelman developed their assays for CHIK and ONNV, the results should help improve the design of ssqPCR assays for the study of other RNA viruses as well.


'/>"/>

Contact: Susan Trulove
STrulove@vt.edu
540-231-5646
Virginia Tech
Source:Eurekalert

Related biology news :

1. Rensselaer leads effort to replace 1 of the most widely used drugs in American hospitals
2. Features of replication suggest viruses have common themes, vulnerabilities
3. First all-African GM crop is resistant to maize streak virus
4. Carnegie Mellon scientist uses mass spectrometer to weigh virus particle, von Willebrand factor
5. Gamma globulin effective in treating eye infections caused by adenoviruses
6. Discovery may help defang viruses
7. Who will recover spontaneously from hepatitis C virus infection
8. Prions and retroviruses -- an unholy alliance?
9. Scientists learn structure of enzyme in unusual virus
10. UGA Odum School of Ecology professor receives grant to study West Nile Virus in NYC
11. New field-deployable biosensor detects avian influenza virus in minutes instead of days
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/12/2017)... 12, 2017  New research undertaken by Fit Small ... future.  1,000 participants were simply asked which office technology had ... may consider standard issue.  Insights on what will ... gathered from futurists and industry leaders including Penelope Trunk ... .  Some of these findings ...
(Date:1/11/2017)... Johnson, co-founder of Visikol Inc. a company originally funded with an ... the elite "Forbes 30 Under 30" list in the Science category. ... fields nationwide to be recognized as a leader in business and ... ... PhD candidate at Rutgers University. Visikol , ...
(Date:1/6/2017)... , Jan. 5, 2017  Delta ID Inc., a ... scanning technology for automotive at CES® 2017. Delta ID ... ) to demonstrate the use of iris scanning as ... authenticate the driver in a car, and as a ... driving experience. Delta ID and Gentex will ...
Breaking Biology News(10 mins):
(Date:1/17/2017)... 2017 The Global Implantable Biomaterials Market ... around 7.5% over the next decade to reach ... prominent trends that the market is witnessing include ... graft transplant surgeries and medical implants and technological ... categorized into immunomodulatory biomaterials, natural, polymers, hydrogels and ...
(Date:1/17/2017)... , Jan. 17, 2017  Zimmer Biomet Holdings, ... quarter and full-year 2016 sales and earnings conference call ... January 31, 2017, at 8 a.m. Eastern Time.  A ... be made available at 7:30 a.m. Eastern Time the ... audio webcast can be accessed via Zimmer Biomet,s Investor ...
(Date:1/17/2017)... Jan. 17, 2017 Research and Markets ... "Molecular Diagnostics - Technologies, Markets and Companies" to their ... ... increased remarkably during the past few years. More than 1,000 ... diagnostics and 342 of these are profiled in the report ...
(Date:1/17/2017)... Cleveleys, UK (PRWEB) , ... ... ... results presented by surgeons at the 2016 annual meeting of the North ... beneficial clinical outcomes, for patients in the majority of cases, when PEEK-OPTIMA™ ...
Breaking Biology Technology: