Navigation Links
Widely used virus assay shown unreliable when compared to other methods
Date:10/21/2009

Blacksburg, Va. -- In the course of doing research on the mosquito-borne pathogens chikungunya virus (CHIKV) and o' nyong-nyong virus (ONNV), Virginia Tech researchers have discovered an inconvenient truth about an assay, strand-specific quantitative real-time PCR (ssqPCR), increasingly being used to detect and measure replicating viral RNA in infected cells and tissues. The method most labs are using for ssqPCR is unreliable.

The research appears in the Wednesday, October 14, 2009, issue of PLoS ONE, in the article, "Accurate Strand-Specific Quantification of Viral RNA," by Nicole E. Plaskon of Richmond Va., a Master of Science in life sciences candidate in the College of Agriculture and Life Sciences, and entomology Assistant Professors Zach N. Adelman and Kevin M. Myles, all with the Fralin Life Science Institute..

CHIKV has sickened millions of people in India and Africa in the last five years 1.3 million in India alone. ONNV has also previously caused large outbreaks of human disease with cases numbering in the millions. In studying virus infection of the mosquito, the Virginia Tech researchers developed a novel assay that detects and measures anti-genomic copies of the viral genome. This differs from traditional assays that simply measure viral nucleic acids associated with infection, regardless of origin.

"The application of real-time PCR to the detection and quantification of specific strands of viral RNA is becoming an increasingly important tool in the study of RNA viruses. As a result, multiple types of ssqPCR assays have been described and are in widespread use. However, no study has yet determined if the accuracy with which the different types of assays detect and quantify specific strands of viral RNA are equivalent. It turns out they are not, and the most frequently used method is the most error prone," said Myles.

"A less frequently used ssqPCR assay turned out to be more accurate," said Adelman.


'/>"/>
Contact: Susan Trulove
STrulove@vt.edu
540-231-5646
Virginia Tech
Source:Eurekalert

Page: 1 2

Related biology news :

1. Rensselaer leads effort to replace 1 of the most widely used drugs in American hospitals
2. Features of replication suggest viruses have common themes, vulnerabilities
3. First all-African GM crop is resistant to maize streak virus
4. Carnegie Mellon scientist uses mass spectrometer to weigh virus particle, von Willebrand factor
5. Gamma globulin effective in treating eye infections caused by adenoviruses
6. Discovery may help defang viruses
7. Who will recover spontaneously from hepatitis C virus infection
8. Prions and retroviruses -- an unholy alliance?
9. Scientists learn structure of enzyme in unusual virus
10. UGA Odum School of Ecology professor receives grant to study West Nile Virus in NYC
11. New field-deployable biosensor detects avian influenza virus in minutes instead of days
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/18/2014)... for evaluation of suspected genetic conditions, whole-exome sequencing ... including detection of a number of rare genetic ... to a study appearing in JAMA . ... the American Society of Human Genetics annual meeting. ... regions of thousands of genes simultaneously using next-generation ...
(Date:10/17/2014)... for the first time how bacteria can grow directly ... the opportunity to get tremendous insights into bacteria behavior ... discovered the bacterial growth in chronic lung infections among ... by the immune cells. The researchers discovered the immune ... bacteria, forcing the bacteria to switch to a much ...
(Date:10/16/2014)... land managers have a new synthesis of recent research ... (MMC) forests in the two states. , The ... Oregon and Washington: A Synthesis of the Relevant Biophysical ... technical report published by the U.S. Forest Service,s Pacific ... request from managers for a synthesis of the large ...
Breaking Biology News(10 mins):Whole-exome sequencing shows potential as diagnostic tool 2Scientists opens black box on bacterial growth in cystic fibrosis lung infection 2New report synthesizes best available science on management of moist mixed-conifer forests 2
... Primate Research Center, Oregon Health & Science University, suggests ... counteract some negative impacts of smoking in unborn babies. ... mothers who continue to smoke throughout pregnancy despite physician ... of the American Journal of Respiratory and Critical Care ...
... National Human Genome Research Institute (NHGRI), part of the ... chromosomes have evolved by breaking at specific sites rather ... the breakage hotspots are also involved in human cancer. ... issue of the journal Science, a team of 25 ...
... that a protein prolonging life in mice works by controlling ... species. In mice, the researchers discovered, it acts as a ... Therapies based on this hormone could prove to be a ... Makoto Kuro-o, assistant professor of pathology and senior author of ...
Cached Biology News:OHSU research shows vitamin C counteracts some negative impacts of smoking on unborn babies 2OHSU research shows vitamin C counteracts some negative impacts of smoking on unborn babies 3Multi-species genome comparison sheds new light on evolutionary processes, cancer mutations 2Multi-species genome comparison sheds new light on evolutionary processes, cancer mutations 3Scientists find that protein controls aging by controlling insulin 2
(Date:10/19/2014)... Oct. 19, 2014 NextCODE Health, which enables clinicians ... in real time, today announced the launch of its new ... , at the American Society of Human Genetics (ASHG) annual ... more, sign up and apply for free beta access, visit ... and benefits can be viewed here . ...
(Date:10/19/2014)... 2014 The Asia-Pacific Speech Analytics market ... Asia-Pacific with analysis and forecast of revenue. This market ... to $208 million by 2019, at a Compound Annual ... , Browse through the TOC of the Asia-Pacific Speech ... in-depth analysis provided. It also provides a glimpse of ...
(Date:10/19/2014)... 2014 The report "Unmanned Ground ... Security), Technology, Payload, Type & by Geography - ... global unmanned ground vehicle market into various sub ... market sizes. The report also identifies the factors ... it along with the adoption trends. , The ...
(Date:10/18/2014)... 18, 2014 The Asia-Pacific Bio-based ... segments the bio-based advanced phase change material market ... , Browse through the TOC of the Asia-Pacific ... get an idea of the in-depth analysis provided. ... in the Asia-Pacific bio-based advanced phase change material ...
Breaking Biology Technology:The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 2The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 2The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 3The Asia-Pacific Speech Analytics market is estimated to reach $208 million by 2019 - New Report by MicroMarket Monitor 4Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 2Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 3Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 4Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 5The Asia-Pacific Bio-based Advanced Phase Change Materials Market is estimated to grow to $39.8 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bio-based Advanced Phase Change Materials Market is estimated to grow to $39.8 million by 2018 - New Report by MicroMarket Monitor 3The Asia-Pacific Bio-based Advanced Phase Change Materials Market is estimated to grow to $39.8 million by 2018 - New Report by MicroMarket Monitor 4
... New Deal Advances the Company,s Groundbreaking Smart Shape Memory ... (BIO-SMP(TM)) Commercialization Program ... the pioneer,in the design, manufacture and commercialization of innovative,biocompatible ... global licensing agreement for an application of the,Company,s BIO-SMP(TM) ...
... MELBOURNE, Australia, Jan. 30 Phosphagenics,Limited ("Phosphagenics") (ASX: ... a pre-clinical study demonstrating that the,combination of its ... significantly reduced serum lipids and inflammatory proteins,involved in ... The purpose of the study was to determine ...
... composed of tightly packed nanostructures that resemble tiny nails, ... Laboratories have created a material that can repel almost ... the liquid on the surface slips past the heads ... wetting the surface completely. , The new material, which ...
Cached Biology Technology:mNEMOSCIENCE GmbH Announces Global Licensing Agreement With Aporo Biomedical 2mNEMOSCIENCE GmbH Announces Global Licensing Agreement With Aporo Biomedical 3Phosphagenics Reports Superior Pre-clinical Cholesterol Results from APA-01 and Atorvastatin Combination 2Phosphagenics Reports Superior Pre-clinical Cholesterol Results from APA-01 and Atorvastatin Combination 3Phosphagenics Reports Superior Pre-clinical Cholesterol Results from APA-01 and Atorvastatin Combination 4
... PEP-152 is a 17 amino acid synthetic peptide whose sequence is ... (amino to carboxy terminus): C - N(217) - Q - ... - A - T - P - N - Y - ... may be used for neutralization and control experiments with the polyclonal ...
... for which no antibody exists? Having difficulty generating ... a variety of pharmaceutical, biotech and academic researchers ... technology and expertise in antibody generation to work ... RabMAb development technology , High affinity and high ...
... The Rapid-Screen cDNA Library Panels were designed ... three sets of PCRs to identify the ... is performed in a 96-well "Master Plate," ... 5,000 clones. Having identified the positive well(s) ...
... Library Panels were designed for full-length gene ... PCRs to identify the desired cDNA clone. ... a 96-well "Master Plate," where each well ... identified the positive well(s) by gel electrophoresis ...
Biology Products: