Navigation Links
Widely used virus assay shown unreliable when compared to other methods
Date:10/21/2009

Blacksburg, Va. -- In the course of doing research on the mosquito-borne pathogens chikungunya virus (CHIKV) and o' nyong-nyong virus (ONNV), Virginia Tech researchers have discovered an inconvenient truth about an assay, strand-specific quantitative real-time PCR (ssqPCR), increasingly being used to detect and measure replicating viral RNA in infected cells and tissues. The method most labs are using for ssqPCR is unreliable.

The research appears in the Wednesday, October 14, 2009, issue of PLoS ONE, in the article, "Accurate Strand-Specific Quantification of Viral RNA," by Nicole E. Plaskon of Richmond Va., a Master of Science in life sciences candidate in the College of Agriculture and Life Sciences, and entomology Assistant Professors Zach N. Adelman and Kevin M. Myles, all with the Fralin Life Science Institute..

CHIKV has sickened millions of people in India and Africa in the last five years 1.3 million in India alone. ONNV has also previously caused large outbreaks of human disease with cases numbering in the millions. In studying virus infection of the mosquito, the Virginia Tech researchers developed a novel assay that detects and measures anti-genomic copies of the viral genome. This differs from traditional assays that simply measure viral nucleic acids associated with infection, regardless of origin.

"The application of real-time PCR to the detection and quantification of specific strands of viral RNA is becoming an increasingly important tool in the study of RNA viruses. As a result, multiple types of ssqPCR assays have been described and are in widespread use. However, no study has yet determined if the accuracy with which the different types of assays detect and quantify specific strands of viral RNA are equivalent. It turns out they are not, and the most frequently used method is the most error prone," said Myles.

"A less frequently used ssqPCR assay turned out to be more accurate," said Adelman.


'/>"/>
Contact: Susan Trulove
STrulove@vt.edu
540-231-5646
Virginia Tech
Source:Eurekalert

Page: 1 2

Related biology news :

1. Rensselaer leads effort to replace 1 of the most widely used drugs in American hospitals
2. Features of replication suggest viruses have common themes, vulnerabilities
3. First all-African GM crop is resistant to maize streak virus
4. Carnegie Mellon scientist uses mass spectrometer to weigh virus particle, von Willebrand factor
5. Gamma globulin effective in treating eye infections caused by adenoviruses
6. Discovery may help defang viruses
7. Who will recover spontaneously from hepatitis C virus infection
8. Prions and retroviruses -- an unholy alliance?
9. Scientists learn structure of enzyme in unusual virus
10. UGA Odum School of Ecology professor receives grant to study West Nile Virus in NYC
11. New field-deployable biosensor detects avian influenza virus in minutes instead of days
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/19/2014)... researchers from several Harvard University labs in collaboration ... aspiring researchers with the intellectual raw materials needed ... soft, flexible materials. , With the advent of ... in manufacturing technology, soft robotics is emerging as ... conventional rigid robot design, but working with pliable ...
(Date:9/19/2014)... biology professor will study multifunctional and specialized spinal ... a National Science Foundation grant in the amount ... Berkowitz, professor in the Department of Biology, OU ... the differences between the multifunctional and specialized spinal ... use and what connections they make. Berkowitz ...
(Date:9/19/2014)... which over thousands of years has adapted to the arctic ... makes the cold dangerous for you. , This is happening ... the cold provoking substance, called nonylphenol, comes from the use ... of being a endocrine disruptor, but when entering the worm ... to protect the cells in its body from cold damage. ...
Breaking Biology News(10 mins):Soft robotics 'toolkit' features everything a robot-maker needs 2Environmental pollutants make worms susceptible to cold 2Environmental pollutants make worms susceptible to cold 3
... aging and many age-related diseases is oxidative stress," says the ... of pathology. The skin of a bitten apple will brown ... that is a good metaphor for the damage that oxidative ... over time. , Humans and other organisms depend on oxygen ...
... Avoiding quicksand along the banks of the Ivindo River in ... patterns of electricity created by fish in the water. ... groups of local electric fish have different DNA, different communication ... have found a case where two types of electric signals ...
... Using a "clot buster" drug normally reserved for treating patients ... double the number of patients who could be revived from ... more than 260,000 people a year nationwide ?and at least ... as a new therapy for this abrupt and catastrophic loss ...
Cached Biology News:Free radical cell death switch identified 2Electric fish in Africa could be example of evolution in action 2Clot-busting drug helps revive cardiac arrest patients 2
(Date:9/22/2014)... -- Neogen Corporation (Nasdaq: NEOG ) announced today ... 2015 fiscal year, which ended Aug. 31, was $8,883,000 ... $7,839,000 in the first quarter of last year. Adjusted ... earnings per share in the current quarter were $0.24, ... quarter revenues increased 15% to $67,599,000, from the previous ...
(Date:9/22/2014)... METTLER TOLEDO is pleased to ... device CNS110 ScanTape™ . Replacing a traditional ... greater ease and accuracy for transport and logistics ... enhance productivity—an important benefit for load planning and ... profits. , Usable at any point in ...
(Date:9/21/2014)... scientists have discovered how to produce ultra-thin "diamond nanothreads" ... than that of today,s strongest nanotubes and polymers. A ... by John V. Badding, a professor of chemistry at ... September 2014 issue of the journal Nature Materials ... discovery is intriguing because the threads we formed have ...
(Date:9/20/2014)... MediVet America and NanoFiber ... surgery at the specialized Wolvega Horse Clinic. , Due ... tendon was ruptured 90% and her deep digital flexor ... death sentence for a horse. , The clinic has ... . This revolutionary regenerative option for ruptured tendons ...
Breaking Biology Technology:Neogen reports 13% net income increase 2Neogen reports 13% net income increase 3Neogen reports 13% net income increase 4Neogen reports 13% net income increase 5Neogen reports 13% net income increase 6Neogen reports 13% net income increase 7ScanTape™ Handheld Dimensioner Increases Processing Volume 2Smallest possible diamonds form ultra-thin nanothreads 2Smallest possible diamonds form ultra-thin nanothreads 3Smallest possible diamonds form ultra-thin nanothreads 4World’s first tendon replacement using Nanofiber and activated MediVet Stem Cell Fraction 2World’s first tendon replacement using Nanofiber and activated MediVet Stem Cell Fraction 3
... to fuel requires several steps: chemical pretreatment to break ... to remove the toxic chemicals required in pretreatment, and ... Virginia Tech researchers have discovered an enzyme mixture that ... biomass (hydrolysate), meaning that the detoxification step is unnecessary, ...
... 2011 Cadence Pharmaceuticals, Inc. (Nasdaq: ... commercializing proprietary product candidates principally for use in the hospital ... and full year ended December 31, 2010 financial results on ... host a conference call and live webcast to discuss the ...
... Bulletin Board: GOVX ), an Atlanta-based biotechnology company ... its financial results for the fourth quarter and year ended ... of $478,784 for the fourth quarter ended December 31, 2010, ... comparable period in 2009. For the full year of 2010, ...
Cached Biology Technology:Enzyme cocktail could eliminate a step in biofuel process 2GeoVax Labs Reports Fourth Quarter and Year-End 2010 Financial Results 2GeoVax Labs Reports Fourth Quarter and Year-End 2010 Financial Results 3GeoVax Labs Reports Fourth Quarter and Year-End 2010 Financial Results 4GeoVax Labs Reports Fourth Quarter and Year-End 2010 Financial Results 5GeoVax Labs Reports Fourth Quarter and Year-End 2010 Financial Results 6
Rabbit polyclonal to Aortic Elastin ( Abpromise for all tested applications). entrezGeneID: 2006 SwissProtID: P15502...
Anti-gp120/160(SIV/mac239) Immunogen: gp120(SIV/mac239) protein (Genebank accession #M33262)...
Tropomyosin 4 [Tm4]...
...
Biology Products: