Navigation Links
Widely used virus assay shown unreliable when compared to other methods
Date:10/21/2009

Blacksburg, Va. -- In the course of doing research on the mosquito-borne pathogens chikungunya virus (CHIKV) and o' nyong-nyong virus (ONNV), Virginia Tech researchers have discovered an inconvenient truth about an assay, strand-specific quantitative real-time PCR (ssqPCR), increasingly being used to detect and measure replicating viral RNA in infected cells and tissues. The method most labs are using for ssqPCR is unreliable.

The research appears in the Wednesday, October 14, 2009, issue of PLoS ONE, in the article, "Accurate Strand-Specific Quantification of Viral RNA," by Nicole E. Plaskon of Richmond Va., a Master of Science in life sciences candidate in the College of Agriculture and Life Sciences, and entomology Assistant Professors Zach N. Adelman and Kevin M. Myles, all with the Fralin Life Science Institute..

CHIKV has sickened millions of people in India and Africa in the last five years 1.3 million in India alone. ONNV has also previously caused large outbreaks of human disease with cases numbering in the millions. In studying virus infection of the mosquito, the Virginia Tech researchers developed a novel assay that detects and measures anti-genomic copies of the viral genome. This differs from traditional assays that simply measure viral nucleic acids associated with infection, regardless of origin.

"The application of real-time PCR to the detection and quantification of specific strands of viral RNA is becoming an increasingly important tool in the study of RNA viruses. As a result, multiple types of ssqPCR assays have been described and are in widespread use. However, no study has yet determined if the accuracy with which the different types of assays detect and quantify specific strands of viral RNA are equivalent. It turns out they are not, and the most frequently used method is the most error prone," said Myles.

"A less frequently used ssqPCR assay turned out to be more accurate," said Adelman.

"The fact that many labs have been using assays prone to error may have led to some wrong conclusions," Adelman said. "Using the more accurate assays will lead to more accurate conclusions and better science."

Although Myles and Adelman developed their assays for CHIK and ONNV, the results should help improve the design of ssqPCR assays for the study of other RNA viruses as well.


'/>"/>

Contact: Susan Trulove
STrulove@vt.edu
540-231-5646
Virginia Tech
Source:Eurekalert

Related biology news :

1. Rensselaer leads effort to replace 1 of the most widely used drugs in American hospitals
2. Features of replication suggest viruses have common themes, vulnerabilities
3. First all-African GM crop is resistant to maize streak virus
4. Carnegie Mellon scientist uses mass spectrometer to weigh virus particle, von Willebrand factor
5. Gamma globulin effective in treating eye infections caused by adenoviruses
6. Discovery may help defang viruses
7. Who will recover spontaneously from hepatitis C virus infection
8. Prions and retroviruses -- an unholy alliance?
9. Scientists learn structure of enzyme in unusual virus
10. UGA Odum School of Ecology professor receives grant to study West Nile Virus in NYC
11. New field-deployable biosensor detects avian influenza virus in minutes instead of days
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/13/2017)... Calif. , April 13, 2017 UBM,s ... York will feature emerging and evolving technology ... Both Innovation Summits will run alongside the expo portion ... speaker sessions, panels and demonstrations focused on trending topics ... largest advanced design and manufacturing event will take place ...
(Date:4/11/2017)... April 11, 2017 No two people ... at the New York University Tandon School of ... have found that partial similarities between prints are ... in mobile phones and other electronic devices can ... The vulnerability lies in the fact that fingerprint-based ...
(Date:4/5/2017)... 5, 2017 Today HYPR Corp. , ... server component of the HYPR platform is officially ... end-to-end security architecture that empowers biometric authentication across Fortune ... already secured over 15 million users across the financial ... connected home product suites and physical access represent a ...
Breaking Biology News(10 mins):
(Date:7/20/2017)... ... 2017 , ... Corista, a leader in integrated pathology solutions, ... 25, during the Association of Pathology Chairs’ Jubilee Meeting in Washington, DC. The ... Director of Pathology Informatics, will present “The Digital Pathology Experience at Johns Hopkins.” ...
(Date:7/18/2017)... ... July 18, 2017 , ... Sourcing custom glass or quartz parts ... has the capabilities to properly execute your job can take many hours of emails, ... a sourcing portal designed to showcase the company’s capabilities and core custom categories, and ...
(Date:7/18/2017)... ... 18, 2017 , ... Recently recognized by CIO Magazine as ... migration of its flagship cloud-based product Planet Life Cycle – a robust work ... system that merges strategic and financial planning with execution. The solution is leveraged ...
(Date:7/17/2017)... ... July 17, 2017 , ... ... a wide range of overlapping clinical features. The advancement of targeted next-generation sequencing ... of NDD research and testing. , However, designing a custom panel for ...
Breaking Biology Technology: