Navigation Links
Widely used virus assay shown unreliable when compared to other methods
Date:10/21/2009

Blacksburg, Va. -- In the course of doing research on the mosquito-borne pathogens chikungunya virus (CHIKV) and o' nyong-nyong virus (ONNV), Virginia Tech researchers have discovered an inconvenient truth about an assay, strand-specific quantitative real-time PCR (ssqPCR), increasingly being used to detect and measure replicating viral RNA in infected cells and tissues. The method most labs are using for ssqPCR is unreliable.

The research appears in the Wednesday, October 14, 2009, issue of PLoS ONE, in the article, "Accurate Strand-Specific Quantification of Viral RNA," by Nicole E. Plaskon of Richmond Va., a Master of Science in life sciences candidate in the College of Agriculture and Life Sciences, and entomology Assistant Professors Zach N. Adelman and Kevin M. Myles, all with the Fralin Life Science Institute..

CHIKV has sickened millions of people in India and Africa in the last five years 1.3 million in India alone. ONNV has also previously caused large outbreaks of human disease with cases numbering in the millions. In studying virus infection of the mosquito, the Virginia Tech researchers developed a novel assay that detects and measures anti-genomic copies of the viral genome. This differs from traditional assays that simply measure viral nucleic acids associated with infection, regardless of origin.

"The application of real-time PCR to the detection and quantification of specific strands of viral RNA is becoming an increasingly important tool in the study of RNA viruses. As a result, multiple types of ssqPCR assays have been described and are in widespread use. However, no study has yet determined if the accuracy with which the different types of assays detect and quantify specific strands of viral RNA are equivalent. It turns out they are not, and the most frequently used method is the most error prone," said Myles.

"A less frequently used ssqPCR assay turned out to be more accurate," said Adelman.


'/>"/>
Contact: Susan Trulove
STrulove@vt.edu
540-231-5646
Virginia Tech
Source:Eurekalert

Page: 1 2

Related biology news :

1. Rensselaer leads effort to replace 1 of the most widely used drugs in American hospitals
2. Features of replication suggest viruses have common themes, vulnerabilities
3. First all-African GM crop is resistant to maize streak virus
4. Carnegie Mellon scientist uses mass spectrometer to weigh virus particle, von Willebrand factor
5. Gamma globulin effective in treating eye infections caused by adenoviruses
6. Discovery may help defang viruses
7. Who will recover spontaneously from hepatitis C virus infection
8. Prions and retroviruses -- an unholy alliance?
9. Scientists learn structure of enzyme in unusual virus
10. UGA Odum School of Ecology professor receives grant to study West Nile Virus in NYC
11. New field-deployable biosensor detects avian influenza virus in minutes instead of days
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/22/2014)... , Dec. 22, 2014  NXT-ID, Inc. (NASDAQ: ... "Company"), a biometric authentication company focused on the growing ... entertainer and security consultant, Apollo Robbins for ... smart wallet.  Apollo Robbins ... 7 th , 2015, demonstrating some of his famous ...
(Date:12/22/2014)... -- The 2014 Holiday Season may be the brightest ... Intelligence reports that the long anticipated floodgates for ... that intensifying demand for smart phones, tablets, and ... a global market of 2.5 billion users with ... According to Maxine Most , ...
(Date:12/19/2014)... 2014 Research and Markets ( http://www.researchandmarkets.com/research/86ncd6/micro_market ... Market Monitor: North America Perimeter Security Systems Market" ... http://photos.prnewswire.com/prnh/20130307/600769 The North American perimeter security ... 3.6% from 2014 to 2019. Although the U.S. market ... Canada is expected to grow at a ...
Breaking Biology News(10 mins):NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4First Season of Holiday Shopping with Mobile Biometric Payments Wraps Up With a Present for Biometrics Industry: A Rosy Forecast for More Than 2 Billion Users of 4.8 Billion Mobile Biometric Devices by 2020 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 2Micro Market Monitor: North America Perimeter Security Systems Market (Intrusion Detection Sensor, Video Surveillance, Communication/Alarm and Notification, Access Control System) 3
... continents have engineered an efficient and environmentally friendly catalyst ... used extensively in modern industry to manufacture fertilizer and ... abundant element, it is generally not found as the ... water (H2O) or to carbon in methane (CH4), the ...
... tool may be able to improve its specificity. ... Nature Biotechnology , Massachusetts General Hospital (MGH) investigators describe ... (gRNA) component of the synthetic enzymes called CRISPR-Cas RNA-guided ... mutations at sites other than the intended target, a ...
... have discovered that breast stem cells and their ,daughters, ... are active in puberty and throughout life. The ... that they could harbour genetic defects or damage that ... timeline of breast cancer development. The finding is also ...
Cached Biology News:Engineers teach old chemical new tricks to make cleaner fuels, fertilizers 2Engineers teach old chemical new tricks to make cleaner fuels, fertilizers 3Engineers teach old chemical new tricks to make cleaner fuels, fertilizers 4Shortening guide RNA markedly improves specificity of CRISPR-Cas nucleases 2Long-lived breast stem cells could retain cancer legacy 2
(Date:12/22/2014)... HILL, N.C. , Dec. 22, 2014 /PRNewswire/ ... today announced that it has signed a letter ... company that has developed and patented a nanotechnology-based ... medical diagnostics products that enable rapid on-site collection ... alcohol use/abuse and health issues in an immediate, ...
(Date:12/22/2014)... MISSION VIEJO, Calif. , Dec. 22, 2014 /PRNewswire/ ... announce that CoPatient, an online medical bill review and ... newest offering in the FREE WellCard Savings discount health ... a medical bill that was more than they expected ... help members reduce health care costs, WellCard Savings is ...
(Date:12/22/2014)... Illinois (PRWEB) December 22, 2014 ITRA ... tenants and occupiers of commercial real estate, has further ... new offices in Perth and Brisbane, Western Australia, reports ... of directors. , ITRA Global / ACORPP (Australian ... totally independent consultancy company providing property services to a ...
(Date:12/19/2014)... OR and Hershey, PA (PRWEB) December 19, 2014 ... Pink: BJCT), a leading developer and manufacturer of ... entered into an agreement with Immunomic Therapeutics, Inc. ... Biojector®-2000 needle-free injection device with its LAMP™ vaccine ... an option for an exclusive Worldwide license to ...
Breaking Biology Technology:ALNE Announces Intention To Acquire BioTechPharma 2WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2ITRA Global Continues International Expansion in Perth and Brisbane, Australia 2Bioject and Immunomic Therapeutics Enter into an Agreement for License of Needle-Free Technology for LAMP-vax Vaccines 2Bioject and Immunomic Therapeutics Enter into an Agreement for License of Needle-Free Technology for LAMP-vax Vaccines 3
... the challenge has been to generate a similarly large ... odors in the real world. A further requirement is ... precision and reproducibility. , In a new study published ... White, Mary AtKisson, John Kauer and colleagues demonstrate a ...
... - Lorus Therapeutics Inc. (TSX:,LOR; AMEX: LRP), a ... pharmaceutical products and technologies for the management,of cancer, ... team,with the appointment of Mr. Peter Korth to ... Korth has previously held senior financial and business ...
... A free white paper for clinical,laboratory managers titled: ... Biotech, Inc. at, http://www.aicompanies.com/anoxomat ., As hospital ... with the ongoing challenge of quickly determining the ... As a result, clinical laboratories,face growing pressure to ...
Cached Biology Technology:Lorus Therapeutics Appoints Peter Korth as Chief Financial Officer 2Lorus Therapeutics Appoints Peter Korth as Chief Financial Officer 3Free White Paper Available for Clinical Laboratory Managers: 'Effectively Isolating Anaerobic Bacteria' 2
Goat polyclonal to SPFH2 ( Abpromise for all tested applications). Antigen: Synthetic peptide: EDEPLETATKEN, corresponding to C terminal amino acids 328-339 of Human SPFH2 Entrez Gene ID: 11...
Anti-Kusabira Orange Monoclonal Antibody Description: 100 g Research Focus: tag Storage: -20C Shipping Temperature: 4C...
alpha/beta-Tubulin Antibody Ship: Hot Store: -20 C...
Anti-Kusabira Orange Monoclonal Antibody Description: 100 g Research Focus: tag Storage: -20C Shipping Temperature: 4C...
Biology Products: