Using molecular biological tools, the scientists detected the transmission of a long-distance signal, a "call for help," from the leaves to the roots in the plants that had Bacillus in the soil. The roots responded by secreting a carbon-rich chemical--malic acid.
All plants biosynthesize malic acid, Bais explains, but only under specific conditions and for a specific purpose--in this case, the chemical was actively secreted to attract Bacillus. Magnified images of the roots and leaves showed the ratcheted-up defense response provided by the beneficial microorganisms.
Czymmek captured the definitive proof using a state-of-the-art LSM 510 DUO laser scanning confocal microscope in UD's Bio-Imaging Center. UD is among only a few universities that own one of these million-dollar instruments.
"A plant is a challenge to image because at least half of it is below ground in the form of roots," Czymmek notes. "Here at UD, we use modern technologies including hydroponic growth systems with see-through chambers and sophisticated optical techniques that will enhance the image clarity when visualizing plants and the pathogens attacking them."
Bais and his colleagues are now working to determine what the aerial signal is from the infected leaf to the root using different pathogen-associated molecular markers (PAMPs).
The research not only sheds light on the remarkable signaling system in plants, but also is important to understand how invasive plants conquer new territory with the aid of plant microbes.
"Plants can't move from where they are, so the only way they can accrue good neighbors is through chemistry," Bais notes.
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| Contact: Tracey Bryant tbryant@udel.edu 302-831-8185 University of Delaware Source:Eurekalert |