Navigation Links
When inflexibility is counterproductive: Mechanism of UV-induced DNA Dewar lesion revealed
Date:11/28/2011

Excessive exposure to ultraviolet (UV) radiation of sunlight can result in skin damage and may even induce skin cancers. Irradiation with UV light causes mutations in the DNA, which can interfere with or even inhibit the read-out of genetic information and hence affect the cell function. The Dewar lesion is one of the major UV-induced reaction products, which can itself generate mutations. Understanding the mechanism that leads to the formation of the Dewar lesion is therefore of great interest.Researchers from Ludwig-Maximilians-Universitaet (LMU) in Munich have now shown that the DNA backbone (the double-stranded scaffold which bears the subunits that encode the genetic information) plays a decisive role in the process. The Dewar lesion can be generated only if the backbone of the DNA is intact. If the DNA strand itself is broken, and therefore more flexible, the Dewar reaction will not take place. The process reveals a surprisingly paradoxical facet of the DNA structure. On the one hand, an unbroken backbone is a prerequisite for DNA function and for cell survival; on the other, the intact backbone favors the formation of Dewar lesions upon exposure to UV, and so facilitates UV-induced mutagenesis. (Angewandte Chemie, 23 November 2011)

UV radiation induces molecular changes in DNA structure, which can lead to genetic mutations and finally to cell death. Energetic UV light primarily produces two types of photochemical damage in the subunits of the DNA - cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts. Both types of lesion are due to cross-linking of adjacent pyrimidine bases on the same DNA strand. Continued exposure to UV light transforms the (6-4) photoproduct into a Dewar lesion by inducing further structural changes. Dewar lesions are stable end-products of continuous exposure to sunlight. Moreover, they are highly mutagenic, i.e. they can themselves induce a range of further mutations. "While the chemical changes that give rise to CPDs and (6-4) photoproducts are already well understood, this is not true for the Dewar lesion," says LMU chemist Professor Thomas Carell, who is also a member of the Center for Integrated Protein Science Munich (CiPSM), one of the Clusters of Excellence at LMU.

In a joint project within the SFB749 initiative Carells group together with research teams led by LMU physicist Professor Wolfgang Zinth (CiPSM) and Regina de Vivie-Riedle of the Department of Chemistry, could show that the backbone of the DNA plays a crucial role in the formation of the Dewar lesion. The backbone consists of repeating units made up of sugars and phosphates, which link the succession of bases that represent the protein-coding information in the DNA. "To our surprise, we found that the Dewar lesion can be generated only if the backbone in the affected region is intact," Carell explains. "If the continuity of the backbone is interrupted, or if the cross-linked base-pairs alone are exposed to sunlight, the Dewar structure fails to form." Thus, an interdisciplinary cooperation, which included chemists, physicists and theorists has, for the first time, been able to dissect the photochemical formation of the Dewar lesion at the atomic level. "Our results also show that the process is remarkably effective; indeed, this is one of the most efficient light-induced reactions known to occur within the DNA," says physicist Wolfgang Zinth.

Theoretical considerations yielded further insights into the details of the Dewar isomerization. "To follow the photochemical reaction dynamics on a high level of theory we came up with a hybrid method that separates the molecular system into subsystems treated on different quantum mechanical levels. This hierarchic strategy allows us to evaluate the dynamics of the complete system," says de Vivie-Riedle. Based on these calculations, the researchers were able precisely to define the role of the DNA backbone in the formation of the Dewar lesion. Cleavage of the backbone makes the molecule more flexible. Under these conditions, the (6-4) lesion will be protected and the system returns via a photophysical pathway back to its initial state. In contrast, an intact backbone keeps the molecule rigid, and strains the pyrimidine ring structure. The result is that only those atoms that must rearrange to form the Dewar isomer remain mobile, which favors the reaction that leads to the stable Dewar lesion.


'/>"/>

Contact: Luise Dirscherl
dirscherl@lmu.de
49-892-180-2706
Ludwig-Maximilians-Universitt Mnchen
Source:Eurekalert

Related biology news :

1. Conaway Lab identifies novel mechanism for regulation of gene expression
2. Rong Li Lab probes mechanism of asymmetry in meiotic cell division
3. Newly-discovered mechanism can explain the Beckwith-Wiedemann syndrome
4. Scientists unveil mechanism for up and down in plants
5. New cell division mechanism discovered
6. Corn researchers discover novel gene shut-off mechanisms
7. New regulatory mechanism discovered for cell identify and behavior in forming organs
8. Biomedical engineers detective work reveals antibiotic mechanism
9. Mechanisms of cardiovascular disease and cancer give clues to new therapies
10. Argonne scientists discover possible mechanism for creating handedness in biological molecules
11. A little wine boosts omega-3 in the body: Researchers find a novel mechanism for a healthier heart
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/24/2017)... Controller General of Immigration from Maldives Mr. Mohamed Anwar and ... international IAIR Award for the most innovative high security ePassport and eGates  ... ... Maldives Immigration Controller General, Mr. Mohamed Anwar ... right) have received the IAIR award for the "Most innovative high security ...
(Date:3/22/2017)... VILNIUS, Lithuania , March 21, 2017 /PRNewswire/ ... identification and object recognition technologies, today announced the ... development kit (SDK), which provides improved facial recognition ... safety cameras on a single computer. The new ... algorithms to improve accuracy, and it utilizes a ...
(Date:3/20/2017)... PMD Healthcare announces the release of its ... System (WMS), a remote, real-time lung health monitoring and ... is a Medical Device, Digital Health, and Chronic Care ... innovative solutions that empower people to improve their healthcare ... developed the first ever personal spirometer, Spiro PD, which ...
Breaking Biology News(10 mins):
(Date:5/24/2017)... As Ebola resurfaces in the Democratic ... suspected cases now reported, a new analysis of the Ebola ... correlation between the 2014 and 2017 outbreaks of the disease.  ... 2012-13, which preceded the 2014 outbreak. An analysis of the ... counts in 2014-15, which again precedes the current outbreak in ...
(Date:5/23/2017)... ... May 23, 2017 , ... ... month its 20th anniversary, marking the occasion with a strong presence at Bio-IT ... Welcome Reception and further extends an invitation to all attendees to view ...
(Date:5/23/2017)... ... May 23, 2017 , ... ... technology applications, has announced a facility expansion to accommodate its rapid growth. , ... of new workspace and renovation of the existing areas. The expansion includes, a ...
(Date:5/23/2017)... Switzerland (PRWEB) , ... May 23, 2017 , ... ... forces machine manufacturers to re-engineer their control technology again and again. METTLER TOLEDO ... problem for machine manufacturers. The videos illustrate how integration of the ACT350 into ...
Breaking Biology Technology: