Researchers from North Carolina State University and the University of Minnesota have found for the first time that genetically identical strains of bacteria can respond very differently to the presence of sugars and other organic molecules in the environment, with some individual bacteria devouring the sugars and others ignoring it.
"This highlights the complexity of bacterial behaviors and their response to environmental conditions, and how much we still need to learn," says Dr. Chase Beisel, an assistant professor of chemical and biomolecular engineering at NC State and senior author of a paper describing the work. "This is one additional piece of the puzzle that could help us understand the behaviors of bacterial pathogens or the population dynamics of the micro-organisms that live in our guts."
The researchers grew a non-pathogenic strain of E. coli in liquid culture, with each culture rich in a different type of sugar. Bacteria produce different protein pumps and enzymes that are dedicated to taking in and breaking down specific types of sugar, but only when the relevant sugar is present.
To explore how individual bacteria respond to different sugars, the research team genetically modified the bacteria to create fluorescent proteins whenever they produced the pumps or enzymes. These fluorescent proteins acted as visual cues that the researchers could use to see how individual bacteria responded to the presence of a particular sugar.
The researchers tested the E. coli's responses to eight different sugars. For four of those sugars, all of the bacteria responded identically, increasing consumption in response to greater concentrations of sugar molecules in the culture.
But the bacteria responded differently to the other four molecules, exhibiting a wide range of behaviors.
"The behaviors were all over the place," Beisel says.
"While this is the first time we've seen such diverg
|Contact: Matt Shipman|
North Carolina State University