Navigation Links
Weird, ultra-small microbes turn up in acidic mine drainage

In the depths of a former copper mine in Northern California dwell what may be the smallest, most stripped-down forms of life ever discovered.

The microbes members of the domain of one-celled creatures called Archaea are smaller than other known microorganisms, rivaled in size only by a microbe that can survive solely as a parasite attached to the outside of other cells. Their genomes, reconstructed by a group at the University of California, Berkeley, are among the smallest ever reported.

The researchers also discovered another mine-dwelling microbe that occasionally produces weird protuberances unlike any structures seen before in Archaea and uses them to penetrate the ultra-small microbes.

"Other cells in the mine have what looks like a needle that sometimes pokes right into the cells," said Brett J. Baker, a researcher in UC Berkeley's Department of Earth and Planetary Science and first author of a new paper describing the findings. "It is really remarkable and suggests an interaction that has never been described before in nature."

These cellular extensions are only present when this interaction between the microbes is seen, noted co-author Luis R. Comolli, a microscopist at Lawrence Berkeley National Laboratory (LBNL).

Baker, Comolli and a team led by Jillian Banfield, UC Berkeley professor of earth and planetary science and of environmental science, policy and management and staff scientist at LBNL, published their findings last week in the online early edition of the journal Proceedings of the National Academy of Sciences.

Under a light microscope, the ultra-small microbes look like specks of dust. But Comolli used a state-of-the-art cryoelectron microscope, or cryoEM, to obtain high-resolution, 3-D images and even measure an individual microbe's internal volume between one-tenth and one-hundredth the volume of an E. coli bacterium. Each of the microbes, dubbed ARMAN, for archaeal Richmond Mine acidophilic nanoorganisms, is ellipsoidal and only 200-400 nanometers in diameter, one-third the diameter of the rod-shaped E. coli.

The team reconstructed the genomes of three distinct lineages of ARMAN and found them to be tiny a mere 1 million base pairs, in contrast to hundreds of billions in humans. In the smallest of the three, the average gene length is only 774 base pairs, in contrast to the average gene length in humans of 10,000 to 15,000 base pairs. Base pairs, the smallest chemical units of the gene, are nucleic acids that come in four forms. The base pairs are chained together to make DNA, and a gene is a sequence of base-pairs coding for a unique protein.

The genomes are so small that the researchers initially suspected that the ARMAN microbes are parasites upon other microbes, since parasites can afford to lose genes that their host already has.

But of the 70 individual specimens so far imaged in 3-D, 90 percent seem to be free-living. The other 10 percent are impaled on the mysterious needle-like spines of Thermoplasmatales, the other Archaea living alongside ARMAN in the mine. The researchers suspect that the penetrating spines may mean that the microbes live off other microbes at least part of the time, unlike symbiotic organisms or parasites, which must always associate with other organisms to live.

"ARMAN are among the smallest microbes we know of that, if not free-living, are at least not permanently obliged to be a parasite or symbiont," Comolli said.

The cells are about as large as the largest viruses, which can replicate only in living organisms and are not considered to be "living."

"The genome is very compact," Baker added." A microbial genome 10 percent larger has the same number of genes as ARMAN."

The organism has a much higher percentage 45 percent of unknown genes than any other organism sequenced, he said.

"ARMAN share a lot of genes with Euryarchaeota and Crenarchaeaota, but they also have a lot of genes not seen before in these branches of Archaea," he said, suggesting that ARMAN may have been around since these two branches split billions of years ago.

Three-dimensional cryoEM tomographic reconstructions show the unique architecture of ARMAN, Comolli said. It has very few ribosomes the machines that build proteins per unit volume, for example; in the same volume, E coli would have 100 times more. The ribosomes also are distributed close to the cell wall. ARMAN cells also have an enigmatic internal tube. Like other Archaea, however, they have no nucleus or other internal organelles.

Banfield's group first described the ARMAN microbes four years ago, after identifying the organisms in acidic pools in the Richmond Mine, which is owned by Ted Arman, in Iron Mountain, Calif. The team's continued analysis has revealed amazing organization within the mine drainage biofilm communities that grow on solutions with the acidity of battery acid. The new data will help the researchers explore even further the community of organisms in the mine and determine how the organisms are able to live in such harsh environs and convert iron sulfides to sulfuric acid.

"Having these microbes described at the genomic level allows us to develop molecular identification methods and combine these methods with a 3-D view of the microbes to study the distribution of these organisms within this little ecological system, this little society, in the mine," Comolli said.


Contact: Robert Sanders
University of California - Berkeley

Related biology news :

1. Microbes contribute less to climate warming
2. Earth microbes may contaminate the search for life on Mars
3. Soil microbes produce less atmospheric CO2 than expected with climate warming
4. Explorers census hard-to-see sea life: microbes, tiny animals key to Earths food, carbon systems
5. Dormant microbes promote diversity, serve environment
6. Microbes produce fuels directly from biomass
7. Hot microbes cause groundwater cleanup rethink
8. Marine microbes creating green waves in industry
9. Marine microbes creating green waves in industry
10. Microbes and their hosts -- exploring the complexity of symbiosis in DNA and cell biology
11. Methane-eating microbes can use iron and manganese oxides to breathe
Post Your Comments:
Related Image:
Weird, ultra-small microbes turn up in acidic mine drainage
(Date:11/16/2015)... SAN JOSE, Calif. , Nov 16, 2015 ... leading developer of human interface solutions, today announced ... new Synaptics TouchView ™ touch controller and ... the architectural revolution of smartphones. These new TDDI ... and include TD4100 (HD resolution), TD4302 (WQHD resolution), ...
(Date:11/11/2015)... , Nov. 11, 2015   MedNet Solutions , an ... of clinical research, is pleased to announce that it will ... Trials (PCT) event, to be held November 17-19 in ... to view live demonstrations of iMedNet , ... how iMedNet has been able to deliver time ...
(Date:11/9/2015)... Calif. , Nov. 9, 2015  Synaptics Inc. ... interface solutions, today announced broader entry into the automotive ... solutions that match the pace of consumer electronics human ... biometric sensors are ideal for the automotive industry and ... Europe , ...
Breaking Biology News(10 mins):
(Date:11/27/2015)... ... November 27, 2015 , ... ... that includes over 2,000 technical presentations offered in symposia, oral sessions, workshops, awards, ... spectroscopy, covers a wide range of applications such as, but not limited to, ...
(Date:11/26/2015)... , November 26, 2015 /PRNewswire/ ... --> Accutest Research Laboratories, ... Contract Research Organization (CRO), has formed ... Cancer Center - Temple Health for ... ,     (Photo: ) , ...
(Date:11/25/2015)... November 26, 2015 ... Market 2016 - 2020 report analyzes that automating ... and quality in long-term samples, minimizing manual errors, ... Automation minimizes manual errors such as mislabeling or ... Further, it plays a vital role in blood ...
(Date:11/25/2015)... 2 nouvelles études permettent d , identifier ... souches bactériennes retrouvées dans la plaque dentaire des ... Ces recherches  ouvrent une nouvelle voie ... l,un des problèmes de santé les plus fréquemm ... --> 2 nouvelles études permettent d , ...
Breaking Biology Technology: