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Waves of Berkeley Lab responders deploy omics to track Deepwater Horizon cleanup microbes
Date:6/21/2012

ng the metagenomic, metatranscriptomic and single-cell sequencing techniques accessed through the collaboration with the DOE JGI provided a uniquely comprehensive perspective on the findings. "When we compared the RNA to the DNA we saw enrichment in the same kinds of genes for certain compounds. For example, the genes for degradation of cyclohexane were very abundant in the plume and they were being expressed. It was essential to have the expression data because without that, the DNA might be there but we'd be at a loss to determine whether there was any relevant functional activity," she said. "This shows what's really going on when and where you take your samples. We found that the genes were there, that specific classes of genes were highly expressed, and then they were also found in the single cell that we sequencedall together it made for a really coherent story that can serve as a model for future studies."


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Contact: David Gilbert
degilbert@lbl.gov
924-296-5643
DOE/Joint Genome Institute
Source:Eurekalert

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