Navigation Links
WPI research shows how cranberry juice fights bacteria at the molecular level

WORCESTER, Mass. -- Revealing the science behind the homespun advice, a team of researchers at Worcester Polytechnic Institute (WPI) has identified and measured the molecular forces that enable cranberry juice to fight off urinary tract infections in people.

The data is reported in the paper "Direct adhesion force measurements between E. coli and human uroepithelial cells in cranberry juice cocktail," which was published on-line, ahead of print, by the journal Molecular Nutrition & Food Research. The research illuminates the basic mechanics of E.coli infections, which has implications for developing new antibiotic drugs and infection-resistant materials for invasive medical devices.

The research team led by Terri Camesano, professor of chemical engineering at WPI, focuses on the virulent form of E. coli bacteria that is the primary cause of most urinary tract infections. This strain of E. coli is covered with small hair-like projections known as fimbriae, which act like hooks and latch onto cells that line the urinary tract. When enough of the virulent E. coli adhere to cells in this way, they cause an infection. Previous work by Camesano has shown that exposure to cranberry juice causes the fimbriae on E. coli to curl up, reducing their ability to attach to urinary tract cells. In the new study, Camesano's team presents the first specific measurements of the mechanical forces involved in the attachment of the virulent E. coli to human urinary tract cells. The study also documents how the force of attachment is reduced in the presence of cranberry juice cocktail. "This is not a clinical studyit's a mechanical study that shows us the direct forces that can lead to infection," Camesano said.

To make measurements at the molecular level, Camesano's team developed a method to attach a single E. coli cell to the tip of a probe mounted on a device called an atomic force microscope (AFM). The probe was then dipped in a solution containing human uroepithelial cells, which line the urinary tract. The fimbriae on the E. coli latched onto to specific structures on the human cells, similar to the way the two halves of a Velcro fastener come together. The probe on the AFM was then pulled away from the human cells, measuring the amount of force needed to tear the E. coli away. "We know, on average, how many fimbriae are on each E. coli cell. And the total force we measured correlates with that number. So the data lead us to believe that the fimbriae each bind to a specific receptor on the uroepithelial cells," Camesano said.

The experiment was repeated numerous times with solutions containing human cells and various concentrations of commercially available cranberry juice cocktail. The data showed that the attachment force of the virulent E. coli weakened as the amount of cranberry juice cocktail increased. The study also showed that a strain of E. coli without fimbriae did not bind well to the human urinary tract cells, regardless of the concentration of cranberry juice cocktail, providing further evidence that fimbriae are essential for infection.

Furthermore, Camesano's team found that in the absence of cranberry juice, the strength of the virulent E. coli's bond to the human cells was so strong that it could not be broken by the typical force of urine flowing through a person's urinary tract. However, as the cranberry juice concentration increased, the bond weakened to the point where the E. coli could be stripped away by the force of flowing urine. "The shear force created by flowing urine is a defense mechanism against urinary tract infection," Camesano said.

Since bacterial adhesion is required for infection, Camesano said understanding the specific mechanisms and forces involved will help direct future studies aimed at identifying potential drug targets for new antibiotics. The data may also be useful in studies aimed at engineering the surfaces of invasive medical devices like catheters to make them more resistant to bacterial adhesion.


Contact: Michael Cohen
Worcester Polytechnic Institute

Related biology news :

1. LSU researcher looks for new ways to keep a dirty situation clean
2. Grant to fund research into eradicating the leukemia stem cell
3. Montana State researchers say Triceratops, Torsaurus were same dinosaur at different stages
4. UCI receives prestigious federal research award to hasten medical advances
5. International AIDS Society announces inaugural recipients of innovative HIV research awards
6. New research on rapidly-disappearing ancient plant offers hope for species recovery
7. CWRU dental researchers discover human beta defensins-3 ignite in oral cancer growth
8. In the ring: Researchers fighting bacterial infections zero in on microorganisms soft spots
9. The Samuel Waxman Cancer Research Foundation awards $1.54 million in research grants for 2010-2011
10. InQ Biosciences Introduces First Fully Integrated Cell Research System
11. Penn State to lead new international Center Of Excellence for malaria research
Post Your Comments:
Related Image:
WPI research shows how cranberry juice fights bacteria at the molecular level
(Date:11/17/2015)... 2015  Vigilant Solutions announces today that Mr. ... Directors. --> --> ... the partnership at TPG Capital, one of the largest ... Billion in revenue.  He founded and led TPG,s Operating ... companies, from 1997 to 2013.  In his first role, ...
(Date:11/16/2015)... Nov 16, 2015  Synaptics Inc. (NASDAQ: ... solutions, today announced expansion of its TDDI product ... touch controller and display driver integration (TDDI) solutions ... These new TDDI products add to the previously-announced ... TD4302 (WQHD resolution), and TD4322 (FHD resolution) solutions. ...
(Date:11/12/2015)... Mass. , Nov. 12, 2015  Arxspan ... Institute of MIT and Harvard for use of ... discovery information management tools. The partnership will support ... both biological and chemical research information internally and ... will be used for managing the Institute,s electronic ...
Breaking Biology News(10 mins):
(Date:11/24/2015)... PHILADELPIA, PA (PRWEB) , ... November 24, 2015 , ... ... young entrepreneurs at competitive events in five states to develop and pitch their BIG ... student projects from each state are competing for votes to win the title of ...
(Date:11/24/2015)... , Nov. 24, 2015 /PRNewswire/ - Aeterna Zentaris ... today that the remaining 11,000 post-share consolidation (or ... Warrants (the "Series B Warrants") subject to the ... on November 23, 2015, which will result in ... giving effect to the issuance of such shares, ...
(Date:11/24/2015)... Telbec/ - ProMetic Life Sciences Inc. (TSX: PLI) (OTCQX: PFSCF) ... Laurin , President and Chief Executive Officer of ProMetic, will ... 27 th Annual Healthcare Conference to be held at ... st , at 8.50am (ET) and ProMetic,s management ... The presentation will be available live via a webcast accessible ...
(Date:11/24/2015)... (PRWEB) , ... November 24, 2015 , ... Whitehouse Laboratories ... Laboratory. The new stand-alone facility will be strictly dedicated to basic USP 61, ... and existing clients the chance to have complete chemistry and micro testing performed by ...
Breaking Biology Technology: