Navigation Links
Viruses with integrated gene switch
Date:8/21/2012

Numerous viruses are used in the service of science today. They serve as gene taxis to transfer therapeutic genes into body cells or as therapeutic viruses targeted to infect and destroy cancer cells. For such applications, the viruses are often equipped with additional genes, such as for immune mediators or for proteins inducing programmed cell death. However, these gene products can harm the body if they are released at the wrong moment or at excessive levels. "Ideally, we want to be able to turn on and off the transferred genes at a specific time," says Dr. Dirk Nettelbeck, a virologist from DKFZ.

To this end, Patrick Ketzer of Nettelbeck's group experimented, jointly with colleagues from Konstanz University, with what are called RNA switches. In order to construct such a switch, the researchers inserted synthetic segments of DNA into the viral genetic material in the direct vicinity of the transferred gene. In the infected cell, this construct is transcribed together with the transferred gene into a single messenger RNA (mRNA) molecule. The switch is operated using an agent which is added to cells infected with the virus. The substance is precisely fitted to bind to the RNA molecule and induces it to cut itself up. Thus, the potentially dangerous protein cannot be produced. The researchers copied this regulation mechanism from bacteria which use RNA switches to regulate production of numerous proteins.

The DKFZ virologists first constructed an RNA switch that is kept in permanent "off" position by the substance. The production of the foreign protein does not start as long as substance is added. "This was a first proof that RNA switches work in viruses at all. But it is just as well possible to construct switches that do not allow production of the protein until the substance is added," Dirk Nettelbeck explains.

In cells, it has been possible for many years now to specifically turn on and off genes. To do so, scientists modified
'/>"/>

Contact: Dr. Sibylle Kohlstdt
s.kohlstaedt@dkfz.de
Helmholtz Association of German Research Centres
Source:Eurekalert

Page: 1 2

Related biology news :

1. MBL scientists to explore hidden realm of microbes, viruses beneath the ocean floor
2. Viruses linked to algae that control coral health
3. Flu immunity is affected by how many viruses actually cause the infection
4. Gut microbes battle a common set of viruses shared by global populations
5. Brothers in arms: Commensal bacteria help fight viruses
6. IU role in Human Microbiome Project exposes battle history between bacteria, viruses in human body
7. To spread, nervous system viruses sabotage cell, hijack transportation
8. Berkeley Lab scientists generate electricity from viruses
9. BGI, GMU, Mass. Eye and Ear and OUHSC announce agreement to sequence 100 human adenoviruses
10. GW announces creation of Computational Biology Institute to conduct integrated research
11. The first pilot wastewater treatment plant with integrated wood production opened in Mongolia
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/23/2014)... -- What do mollusks, starfish, and corals have ... they are all calcifiersorganisms that use calcium from ... shells for stability and protection. , The July ... by the Marine Biological Laboratory, addresses the challenges ... worldwide. , As atmospheric carbon dioxide rises, the ...
(Date:7/23/2014)... of getting tangled and forming knots. Scientists study these ... disentangle them (e.g. useful for gene sequencing techniques). Cristian ... (SISSA) in Trieste and his team have been carrying ... their dynamics. In their latest paper, just published in ... Marco Di Stefano, first author and PhD student at ...
(Date:7/23/2014)... exists for Alzheimer,s disease, the devastating neurological disease ... are now reporting new progress on a set ... shows promise as a potential oral therapy for ... of Medicinal Chemistry . , Carlo Ballatore, Kurt ... healthy brain, the protein known as tau binds ...
Breaking Biology News(10 mins):Calcification in changing oceans explored in special issue of The Biological Bulletin 2
... nowhere is this math more evident than in vertebrates, which ... many species do not. Snakes, of course, have no digits, ... a good handle on how these developmental changes are orchestrated ... on birds: Which digits are they: a thumb with index ...
... from the University of California, Davis, and the University of ... the energy-generating structures within cells, divide. The work, which could ... was published today (Sept. 2) in the journal Science ... Jodi Nunnari, professor and chair of molecular cell biology at ...
... two images taken simultaneously with different Envisat sensors of Hurricane Irene, ... black and white image is from Envisat,s radar, covering the coastal ... provides an unusual view of a hurricane as it shows the ... of the image, a dark spot can be seen where the ...
Cached Biology News:New insight in how cells' powerhouse divides 2
(Date:7/23/2014)... Shimadzu Scientific Instruments has launched ... system developed jointly with Ajinomoto Co. Inc. Offering ... 38 amino acids in nine minutes, drastically enhancing ... analysis utilizing a fast application-specific column and an ... results even under conditions with difficult samples that ...
(Date:7/23/2014)... ANTONIO , 23 de julio de 2014 ... (ADBI o la Compañía) ha anunciado hoy una ... Akron (ABIA o el Instituto), para ... ADBI, un dispositivo de terapia de señal bioeléctrica ... El Instituto, que tiene una importante ...
(Date:7/23/2014)... 2014 DuPont Pioneer announced today that ... president of Agricultural Biotechnology (ABT), effective July 21, 2014. ... most recently as president, chief executive officer and board ... in the seed and crop protection industry to this ... Schickler , president of DuPont Pioneer. “I am looking ...
(Date:7/23/2014)... Shire plc (LSE: SHP, ... biopharmaceutical company, and ArmaGen, a US privately held ... collaboration agreement for AGT-182, an investigational enzyme replacement ... the central nervous system (CNS) and somatic manifestations ... collaboration strengthens Shire,s rare disease pipeline of innovative ...
Breaking Biology Technology:Shimadzu's New UF-Amino Station Provides High-Speed, Multi-Component Analysis of Amino Acids in Food & Beverage and Pharmaceutical Testing 2ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 2ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 3ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 4ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 5ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 6DuPont Pioneer Names Neal Gutterson to Lead Agricultural Biotechnology 2DuPont Pioneer Names Neal Gutterson to Lead Agricultural Biotechnology 3Shire Enters Strategic Licensing and Collaboration Agreement With ArmaGen 2Shire Enters Strategic Licensing and Collaboration Agreement With ArmaGen 3Shire Enters Strategic Licensing and Collaboration Agreement With ArmaGen 4Shire Enters Strategic Licensing and Collaboration Agreement With ArmaGen 5
... an improved governance structure, the benefits of nanotechnology may ... not trust the cutting-edge technology, says David Rejeski, director ... on Thursday, April 24, before the Senate subcommittee on ... dark horse in nanotechnologys future, states Rejeski in his ...
... Sangamo,BioSciences, Inc. (Nasdaq: SGMO ) today announced that ... on Wednesday, April 30,2008, after the market closes. The ... 2:00 p.m. PT, which will be open to the ... company will review,the financial results and discuss other business ...
... 23 /PRNewswire-FirstCall/ - Oncolytics Biotech Inc.,("Oncolytics") (TSX:ONC, NASDAQ:ONCY) ... group at St. James,s University Hospital in Leeds,U.K. ... in the May 1, 2008,online issue of The ... Human Dendritic Cells to Promote Innate Antitumor Immunity.", ...
Cached Biology Technology:Improved governance needed to realize nanotech's benefits 2Sangamo BioSciences Announces First Quarter Conference Call and Webcast 2Sangamo BioSciences Announces First Quarter Conference Call and Webcast 3Oncolytics Biotech Inc. Announces Journal of Immunology Publication on Reovirus Activation of Dendritic Cells 2
... a highly purified preparation of the catalytic ... which recognizes the identical cleavage site as ... enzymatic activity (1). Novagen&'s enzyme is produced ... the highest activity available, and is qualified ...
Strip Removal Tool 25/Box...
... The Beacon 2000 Fluorescence Polarization System ... research and assay development. Standard Beacon ... 2000 System uses a quartz-halogen lamp ... of 360-700 nm. It accommodates all ...
... Effectively condenses and traps a wide variety of ... pump oil. Easy-to-use glass condensation flask (GCF400) allows ... contained jar for easy draining. Rapid cooling time ... glass flask for quick solvent changes ...
Biology Products: