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Utility of sequence-related amplified polymorphism (SRAP) markers

hese markers in plant biology. "We found SRAP markers to be comparable to AFLP markers in terms of levels of variation, but requiring significantly less technical effort and cost," says Robarts. "Furthermore, these markers provide highly reproducible results and no prior genomic information is necessary, making them ideal for non-model systems."

The study also suggests these markers will be useful when paired with next-generation sequencing technologies. Because SRAP loci are derived from a single forward primer and numerous reverse primers, it would be possible to adapt the technique to enrich genomic libraries for next-generation sequencing, providing an efficient protocol for discovery of polymorphisms.

"Although these markers have been primarily used for improvement of crop plants, we are excited about the potential of the SRAP marker technique as a more broadly applicable method in plant sciences. We expect these markers to be especially useful for population-level studies, but our results suggest they will likely provide a useful tool at higher taxonomic levels as well," says Robarts.


Contact: Beth Parada
American Journal of Botany

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