Navigation Links
Utility of sequence-related amplified polymorphism (SRAP) markers

Today, many ecological and evolutionary studies depend on a wide range of molecular tools to infer phylogenetic relationships, uncover population structure within species, and track quantitative traits. Agricultural studies use these same tools to improve crop yield and increase resistance to pests and disease.

However, many of these methodssuch as amplified fragment length polymorphisms (AFLP), inter-simple sequence repeats (ISSR), and random amplified polymorphic DNA (RAPD)have technical limitations. These include issues of reproducibility, ambiguity in determining homology, and significant demands on both cost and time for researchers.

Sequence-related amplified polymorphism (SRAP) markers show promise as an alternative to traditional markers as they have proved to be highly variable and less technically demanding to obtain and use. This recently developed dominant marker technique produces genome-wide fragments and has been used primarily in studies aimed at crop development. Previous studies have utilized this method to identify pathogen-resistant markers and better understand the genetic basis of fruit and flower form and structure, as well as flowering and fruiting times.

In a new study in a recent issue of Applications in Plant Sciences, researchers at Ohio State University have made a case for the use of these markers across a broad range of research fields including plant systematics, biogeography, conservation, and ecology. "These markers exhibit variation useful for uncovering genetic structure at a variety of taxonomic levels, constructing linkage maps, and have proven valuable for the improvement of agronomic crops," explains Daniel Robarts, lead author of the study.

Robarts and colleagues surveyed hundreds of published peer-reviewed papers and presented a number of case studies to further demonstrate the applicability of these markers in plant biology. "We found SRAP markers to be comparable to AFLP markers in terms of levels of variation, but requiring significantly less technical effort and cost," says Robarts. "Furthermore, these markers provide highly reproducible results and no prior genomic information is necessary, making them ideal for non-model systems."

The study also suggests these markers will be useful when paired with next-generation sequencing technologies. Because SRAP loci are derived from a single forward primer and numerous reverse primers, it would be possible to adapt the technique to enrich genomic libraries for next-generation sequencing, providing an efficient protocol for discovery of polymorphisms.

"Although these markers have been primarily used for improvement of crop plants, we are excited about the potential of the SRAP marker technique as a more broadly applicable method in plant sciences. We expect these markers to be especially useful for population-level studies, but our results suggest they will likely provide a useful tool at higher taxonomic levels as well," says Robarts.


Contact: Beth Parada
American Journal of Botany

Related biology news :

1. Responses with crizotinib in MET-amplified lung cancer show new targetable form of disease
2. Amplified greenhouse effect shifts norths growing seasons
3. Amplified greenhouse effect shaping North into South
4. Association between glioma susceptibility and XRCC1 Arg399Gln polymorphism
5. Study finds new genetic risk markers in pancreatic cancer
6. Various genes could be used as early biomarkers of stress due to heavy metals
7. Prostate cancer biomarkers identified in seminal fluid
8. Biomarkers accurately distinguish mesothelioma from non-cancerous tissue
9. Eating rice boosts diet quality, reduces body weight and improves markers for health
10. Genetic markers may predict when people with heart disease are likely to have a heart attack
11. Researchers identify new protein markers that may improve understanding of heart disease
Post Your Comments:
(Date:10/29/2015)... YORK , Oct. 29, 2015 ... technology, announced a partnership with 2XU, a global ... to deliver a smart hat with advanced bio-sensing ... and other athletes to monitor key biometrics to ... the strategic partnership, the two companies will bring together ...
(Date:10/27/2015)... October 27, 2015 Munich, ... Gaze Mapping technology (ASGM) automatically maps data from mobile ... Glasses , so that they can be quantitatively ... Munich, Germany , October 28-29, 2015. ... data from mobile eye tracking videos created with ...
(Date:10/26/2015)... 26, 2015 ... adds Biometrics Market Shares, Strategies ... well as Emerging Biometrics Technologies: Global ... its collection of IT and Telecommunications ... --> . ...
Breaking Biology News(10 mins):
(Date:11/30/2015)... (PRWEB) , ... November 30, 2015 , ... ... of a new, Good Manufacturing Practice (GMP) 10000 in the Santiago Marriott. The ... available, and is operated by a world-class team of qualified medical researchers and ...
(Date:11/30/2015)... December 1, 2015 Partnership includes an ... for the u niversity , s ... treatment s cale - up ...   Africa , where licensees based anywhere in the world ... SDN technology. --> Africa , where licensees based anywhere in ...
(Date:11/30/2015)... , Nov. 30, 2015 Spherix ... company committed to the fostering and monetization of ... current and prospective initiatives designed to create shareholder ... Executive Officer of Spherix. "Based on published reports, ... licensees exceeds $50 billion and Spherix will seek ...
(Date:11/30/2015)... 30, 2015 TapImmune, Inc. ... of innovative peptide and gene-based immunotherapeutics and vaccines for ... it will be presenting at the 8 th ... at 2.30 PM PT. Dr. John N. Bonfiglio ... be giving the presentation and will join TapImmune management ...
Breaking Biology Technology: