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Utility of sequence-related amplified polymorphism (SRAP) markers
Date:8/15/2014

Today, many ecological and evolutionary studies depend on a wide range of molecular tools to infer phylogenetic relationships, uncover population structure within species, and track quantitative traits. Agricultural studies use these same tools to improve crop yield and increase resistance to pests and disease.

However, many of these methodssuch as amplified fragment length polymorphisms (AFLP), inter-simple sequence repeats (ISSR), and random amplified polymorphic DNA (RAPD)have technical limitations. These include issues of reproducibility, ambiguity in determining homology, and significant demands on both cost and time for researchers.

Sequence-related amplified polymorphism (SRAP) markers show promise as an alternative to traditional markers as they have proved to be highly variable and less technically demanding to obtain and use. This recently developed dominant marker technique produces genome-wide fragments and has been used primarily in studies aimed at crop development. Previous studies have utilized this method to identify pathogen-resistant markers and better understand the genetic basis of fruit and flower form and structure, as well as flowering and fruiting times.

In a new study in a recent issue of Applications in Plant Sciences, researchers at Ohio State University have made a case for the use of these markers across a broad range of research fields including plant systematics, biogeography, conservation, and ecology. "These markers exhibit variation useful for uncovering genetic structure at a variety of taxonomic levels, constructing linkage maps, and have proven valuable for the improvement of agronomic crops," explains Daniel Robarts, lead author of the study.

Robarts and colleagues surveyed hundreds of published peer-reviewed papers and presented a number of case studies to further demonstrate the applicability of t
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Contact: Beth Parada
apps@botany.org
American Journal of Botany
Source:Eurekalert

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