Navigation Links
Utility of sequence-related amplified polymorphism (SRAP) markers
Date:8/15/2014

Today, many ecological and evolutionary studies depend on a wide range of molecular tools to infer phylogenetic relationships, uncover population structure within species, and track quantitative traits. Agricultural studies use these same tools to improve crop yield and increase resistance to pests and disease.

However, many of these methodssuch as amplified fragment length polymorphisms (AFLP), inter-simple sequence repeats (ISSR), and random amplified polymorphic DNA (RAPD)have technical limitations. These include issues of reproducibility, ambiguity in determining homology, and significant demands on both cost and time for researchers.

Sequence-related amplified polymorphism (SRAP) markers show promise as an alternative to traditional markers as they have proved to be highly variable and less technically demanding to obtain and use. This recently developed dominant marker technique produces genome-wide fragments and has been used primarily in studies aimed at crop development. Previous studies have utilized this method to identify pathogen-resistant markers and better understand the genetic basis of fruit and flower form and structure, as well as flowering and fruiting times.

In a new study in a recent issue of Applications in Plant Sciences, researchers at Ohio State University have made a case for the use of these markers across a broad range of research fields including plant systematics, biogeography, conservation, and ecology. "These markers exhibit variation useful for uncovering genetic structure at a variety of taxonomic levels, constructing linkage maps, and have proven valuable for the improvement of agronomic crops," explains Daniel Robarts, lead author of the study.

Robarts and colleagues surveyed hundreds of published peer-reviewed papers and presented a number of case studies to further demonstrate the applicability of these markers in plant biology. "We found SRAP markers to be comparable to AFLP markers in terms of levels of variation, but requiring significantly less technical effort and cost," says Robarts. "Furthermore, these markers provide highly reproducible results and no prior genomic information is necessary, making them ideal for non-model systems."

The study also suggests these markers will be useful when paired with next-generation sequencing technologies. Because SRAP loci are derived from a single forward primer and numerous reverse primers, it would be possible to adapt the technique to enrich genomic libraries for next-generation sequencing, providing an efficient protocol for discovery of polymorphisms.

"Although these markers have been primarily used for improvement of crop plants, we are excited about the potential of the SRAP marker technique as a more broadly applicable method in plant sciences. We expect these markers to be especially useful for population-level studies, but our results suggest they will likely provide a useful tool at higher taxonomic levels as well," says Robarts.


'/>"/>

Contact: Beth Parada
apps@botany.org
American Journal of Botany
Source:Eurekalert

Related biology news :

1. Responses with crizotinib in MET-amplified lung cancer show new targetable form of disease
2. Amplified greenhouse effect shifts norths growing seasons
3. Amplified greenhouse effect shaping North into South
4. Association between glioma susceptibility and XRCC1 Arg399Gln polymorphism
5. Study finds new genetic risk markers in pancreatic cancer
6. Various genes could be used as early biomarkers of stress due to heavy metals
7. Prostate cancer biomarkers identified in seminal fluid
8. Biomarkers accurately distinguish mesothelioma from non-cancerous tissue
9. Eating rice boosts diet quality, reduces body weight and improves markers for health
10. Genetic markers may predict when people with heart disease are likely to have a heart attack
11. Researchers identify new protein markers that may improve understanding of heart disease
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/20/2016)... Securus Technologies, a leading provider of ... safety, investigation, corrections and monitoring announced that after ... secured the final acceptance by all three (3) ... Systems (MAS) installed. Furthermore, Securus will have contracts ... by October, 2016. MAS distinguishes between legitimate wireless ...
(Date:6/9/2016)... -- Perkotek an innovation leader in attendance control systems is proud to announce the introduction ... to make sure the right employees are actually signing in, and to even control ... ... ... ...
(Date:6/3/2016)... , June 3, 2016 ... Management) von Nepal ... und Lieferung hochsicherer geprägter Kennzeichen, einschließlich Personalisierung, ... führend in der Produktion und Implementierung von ... der Ausschreibung im Januar teilgenommen, aber Decatur ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... 2016 A person commits a crime, and the ... track the criminal down. An outbreak of foodborne ... Administration (FDA) uses DNA evidence to track down the bacteria ... far-fetched? It,s not. The FDA has increasingly used a complex, ... foodborne illnesses. Put as simply as possible, whole genome sequencing ...
(Date:6/23/2016)... Prostate Cancer Foundation (PCF) is pleased to announce 24 new Young Investigator ... Members of the Class of 2016 were selected from a pool of 128 ... About the Class of 2016 PCF Young Investigators ... ... ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... today announced the launch of the Supplyframe Design Lab . Located in ... to explore the future of how hardware projects are designed, built and brought ...
(Date:6/23/2016)... 23, 2016 Apellis Pharmaceuticals, Inc. today ... trials of its complement C3 inhibitor, APL-2. The ... ascending dose studies designed to assess the safety, ... injection in healthy adult volunteers. Forty ... a single dose (ranging from 45 to 1,440mg) ...
Breaking Biology Technology: