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Using plants to silence insect genes in a high-throughput manner
Date:2/2/2012

Ai samples that is four times faster. Many unknown functions of different insect genes involved in the adaptation of insects to their environment can now be analyzed using the VDPS technique.

However, it is still unclear how the individual steps in the RNAi mechanism from producing dsRNA in the plant cell via their uptake in the insect gut to the silencing of the detoxification genes are accomplished to induce a maximum effect. One experiment provided some interesting information: If the enzymatic step which dices dsRNA into small fragments is inhibited in the experimental plants, the amount of transcripts of the detoxification gene was reduced even further. Therefore the plant mediated RNAi procedure may be more effective, if the caterpillars ingest complete dsRNA instead of smaller diced RNA segments. [JWK, AO]


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Contact: Ian T. Baldwin
baldwin@ice.mpg.de
49-364-157-1101
Max Planck Institute for Chemical Ecology
Source:Eurekalert  

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Using plants to silence insect genes in a high-throughput manner
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