Navigation Links
Using plants to silence insect genes in a high-throughput manner
Date:2/2/2012

uences of CYP genes in Manduca sexta and were able to switch off these genes using RNAi technology, but expressed in the plant.

Using plants to silence insect genes

RNA interference (RNAi) is triggered by the production of double-stranded RNA (dsRNA) comprising about 300 base pairs in the cells of tobacco plants. If larvae feed on these plant, the RNA is released in the insect gut. In the experiments, the dsRNA harbored the sequence of the insect gene,CYP6B46, a special cytochrome P450 oxidoreductase specific for Manduca sexta larvae. In a next step, the dsRNA was enzymatically broken down into smaller RNA segments; a special enzyme complex called RISC (RNA-induced silencing complex), which carries several of these RNA segments, specifically binds to the messenger RNA (mRNA) of the CYP6B46 gene and disassembles the mRNA in such a way that the cytochrome P450 enzyme cannot be produced anymore. "We were impressed by the high specificity of these RNAi experiments. The analysis of mRNA transcripts of closely related CYP6 genes revealed that only the CYP6B46 gene was silenced. This means that there was no collateral damage from the procedure: the gene silencing worked on only one targeted gene," says Ian Baldwin.

The use of additional CYP RNAi probes revealed further interesting results: Young caterpillars which had ingested dsRNA of the CYP4M3 gene gained significantly less weight within 14 days in comparison to larvae reared on control plants very likely a consequence of the nicotine and its toxic effect which had been restored by switching off the CYP gene. The RNAi experiments had been conducted using plant viral vectors. Unlike genetically transformed tobacco plants in which CYP dsRNA is produced constitutively, the virus vector-based technique provides dsRNA transiently produced in wildtype tobacco plants. Both methods worked well but the "plant virus-based dsRNA producing system" (VDPS) allows for a throughput of RN
'/>"/>

Contact: Ian T. Baldwin
baldwin@ice.mpg.de
49-364-157-1101
Max Planck Institute for Chemical Ecology
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. NJIT professor finds engineering technique to identify disease-causing genes
2. Research shows that the pill does not deserve its reputation for causing weight gain
3. Planetary "first family" discovered by astronomers using Gemini and Keck Observatories
4. Planetary first family discovered by astronomers using Gemini and Keck Observatories
5. Using water to understand human society, from the industrial revolution to global trade
6. ESA satellites focusing on the Arctic
7. Using the other guys toolkit: Similarities of pumping blood, oil examined
8. Bangladesh Voter Registration Project Now Using MegaMatcher Biometric Technology to Detect and Prevent Duplicate Registrations
9. City of Newark first in nation using cars to power grid
10. Warmer climate causing huge increase in tree mortality across the West
11. Cancer-causing gene discovery suggests new therapies
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Using plants to silence insect genes in a high-throughput manner
(Date:10/16/2014)... severe respiratory tract infections and worldwide claims the ... VIB and Ghent University have succeeded in developing ... virus infection. , Xavier Saelens (VIB/UGent): "We ... way for the development of a novel approach ... suffering in numerous small children and elderly people." ...
(Date:10/15/2014)... book ,Models of Life, is a non-traditional biophysics textbook ... The book is a journey of discovery into biological ... life and biological regulation. It is about how our ... communication between the billions of cells in an organism. ... relationships between principles, which can be found on both ...
(Date:10/15/2014)... 44th Rosenstiel Award for Distinguished Work in Biomedical Science ... mechanisms of genomic instability and its implications for the ... alumnus to win the Rosenstiel Award; the first, Rod ... is the Charles A. Janeway Professor of Pediatrics and ... investigator at the Howard Hughes Medical Institute at Boston ...
Breaking Biology News(10 mins):New perspectives for development of an RSV vaccine 2New book about life as seen from physics 2Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2
... Some parents of children with autism evaluate facial expressions ... a way that is strikingly similar to autistic patients ... Piven of the University of North Carolina at Chapel ... Institute of Technology. Piven, Adolphs and colleague ...
... in individuals of Asian descent increases the risk of ... the August Journal of Lipid Research . In ... this rare variant for apolipoprotein A-V had extremely high ... Apolipoprotein A-V is a recently discovered lipid-binding protein that ...
... BET DAGAN, ISRAEL - Table grapes are subject to serious ... the vine to dinner tables around the world. Mold and ... reduce grape quality during shipping and storage in retail produce ... are one way of ensuring that consumers get fresh, appealing ...
Cached Biology News:UNC, Caltech research finds further evidence for genetic contribution to autism 2UNC, Caltech research finds further evidence for genetic contribution to autism 3Genetic variant increases triglyceride levels in Asian-Americans 2Fresh from the grapevine to the table 2
(Date:10/25/2014)... Los Angeles, California (PRWEB) October 23, 2014 ... VDRM ), a specialty pharmaceutical company devoted to ... pursuing out-licensing partnerships in 2015. , ViaDerma has ... method, which allows for rapid mass transfer of ... into the body to provide immediate localized therapy. ...
(Date:10/25/2014)... Va. (PRWEB) October 24, 2014 ... the nation’s leading nonprofit authority on the ... healthcare information exchange, announces its appointment of four ... Workgroup. The workgroup leaders include:, , ... ,     Erik Pupo, Specialist Leader, Deloitte Consulting ...
(Date:10/25/2014)... 24, 2014 A new way to ... pitfalls of current skin grafting technologies, it also speeds ... The method, outlined in the October issue of STEM ... deep skin injuries that result from severe burns and ... the skin’s regenerative elements. These wounds heal by contraction, ...
(Date:10/25/2014)... , Oct. 24, 2014  RegeneRx Biopharmaceuticals, Inc. (OTCBB: ... presented at the Fourth International Symposium on Thymosins in ... Italy.  "When thymosin beta 4 (TB4) ... into the peritoneal cavity in a rodent model of ... throughout the brain parenchyma (the functional tissue of the ...
Breaking Biology Technology:ViaDerma, Inc. Announces Plans to Pursue Out-Licensing Partnerships in 2015 2ViaDerma, Inc. Announces Plans to Pursue Out-Licensing Partnerships in 2015 3WEDI Appoints Four New Security and Privacy Workgroup Co-chairs 2New engineering method shows promise for faster healing, more cosmetically appealing skin grafts 2New engineering method shows promise for faster healing, more cosmetically appealing skin grafts 3Researchers Report Thymosin Beta 4 Crosses Blood-Brain Barrier In Animal Stroke Model 2
... International,Corporation (Nasdaq: PRXL ) announced today that its ... Common Stock, which will be effected by,means of a ... will receive one additional share of,Common Stock for each ... of,February 22, 2008. The additional shares will be distributed ...
... Incorporated,(Nasdaq: GPRO ) announced today that Carl ... chief operating officer, effective March 1, 2008., ... 2007 as executive vice,president and chief operating officer. ... and development, manufacturing, and,sales and marketing., "Carl,s ...
... Feb. 11 BioSpace, the leading biotech,job board ... over 800,biotechnology and pharmaceutical job seekers to the ... CA., The BioSpace Career Fair attracted a ... diagnostics career,candidates from around southern California. The event ...
Cached Biology Technology:PAREXEL International Announces 2-For-1 Stock Split 2PAREXEL International Announces 2-For-1 Stock Split 3Carl Hull Named President and Chief Operating Officer of Gen-Probe 2Carl Hull Named President and Chief Operating Officer of Gen-Probe 3BioSpace Career Fair in San Diego Draws Record Number of Bioscience Candidates 2
... PureLink PCR Purification Kits provide rapid ... enzymes, short-failed PCR products, and salts from ... bind/wash/elute procedure:, provides two Binding Buffer ... bp* , can be performed in either ...
... PCR Cloning Kit offers the fastest ... cloning of blunt-end PCR products amplified ... includes the linearized and topoisomerase I-activated ... ligations without ligase (1). The pCR-Blunt ...
2.0 GHz Compaq Computer with 512 MB RAM, 32 MB AGP Graphics Card, 40 GB Hard Drive, 1 Serial Port & Open PCI Slot, Windows 2000 OS, MS Office, CD Read/Writer and 18 Flat Panel Monitor...
Blot Paper 7 x 8.5cm semi-dry western blots (order number RB-400)...
Biology Products: