Navigation Links
Using combinatorial libraries to engineer genetic circuits advances synthetic biology
Date:4/22/2009

(Boston) -- Streamlining the construction of synthetic gene networks has led a team of Boston University researchers to develop a technique that couples libraries of diversified components with computer modeling to guide predictable gene network construction without the back and forth tweaking.

By applying engineering principles to biological systems where a set of components can evolve into networks that display desired behaviors known as synthetic biology -- , has led to new opportunities for biofabrication, drug manufacturing -- even potential biofuels.

And while there have been notable successes, the basic process of building and assembling a predictable gene network from bio-molecular parts remains a major challenge that is often frustrating. The time-consuming tweaking phase often requires many months of swapping out different chemical inputs, RNA regulators and promotors before the sought -after network is realized.

In a paper published online this week in Nature Biotechnology, the research team, led by James J. Collins, BU professor of biomedical engineering, focused on ways to speed up the construction process by assembling a library of 20 versions of two gene promotors and a simple synthesis technique to create component libraries for synthetic biology. Each version covered a wide range gene expression. With the activity levels calculated from the component libraries, the scientists turned to a computer model and designed and built a basic gene circuit to predict how fluorescent protein expression varied with levels of promoter-inhibiting chemicals.

Using the same simulation, for the simple gene circuit the researchers went the next step with a genetic timer, a more complicated circuit. However, computer simulation, on its own, was unable to predict the behavior of this timing circuit. They then built a representative genetic timer using a promoter from each of their libraries and, over time, tracked its behavior. Based on information from one network, the research team was able to calibrate their model and achieve accurate predictions from all the other possible network combinations. These timers, the study notes, are effectively genetic toggle switches.

One last test of these genetic timers was to assemble and test one in yeast, which could accurately time yeast sedimentation -- a process that can be applied to biotechnology and some popular brewed beverages.

"The phenotype is crucial in industrial beer, wine and bioethanol fermentation, as it allows for easy removal of yeast sediments after all the sugars have been converted to ethanol," the paper noted.

The researchers concluded that their method using combinatorial libraries to engineer genetic circuits moves the "tweaking" from the back-end of gene network engineering to the front-end.

"Projects undertaken with this approach will help accelerate synthetic biology by yielding many more components for the community," the paper concludes, noting the need for extensive characterization of each component is eliminated or substantially reduced.

"Our work also provides an accessible method for introducing predictable, controlled variability to networks, a feature that is increasingly becoming desirable as synthetic biology enters its second decade."


'/>"/>

Contact: Ronald Rosenberg
ronrosen@bu.edu
617-358-1240
Boston University
Source:Eurekalert

Related biology news :

1. K-State using Second Life island to help high school students learn earth science
2. Caltech scientists control complex nucleation processes using DNA origami seeds
3. VeriLook Surveillance SDK Provides Real-Time Face Identification Using Video Surveillance Cameras
4. VeriLook Surveillance SDK Provides Real-Time Face Identification Using Video Surveillance Cameras
5. Using hair to manage HIV/AIDS and predict treatment success
6. Milestone achieved toward production of malaria treatment using synthetic biology and fermentation
7. MIT: Using touch to help deaf people
8. Dana-Farber oncologists present at ASCO GU -- predict prostate cancer survival using Source MDx test
9. Biological control of tropical weeds using arthropods
10. Defying the disaster: Researcher explores resilient housing
11. Link between unexploded munitions in oceans and cancer-causing toxins determined
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/11/2016)... CHICAGO , Jan. 11, 2016  higi, ... via nearly 10,000 retail locations, web and mobile, ... than $40 million from existing investors. ... will be devoted to further innovate higi,s health ... app and web portal – including expanding services ...
(Date:1/7/2016)... -- This BCC Research report studies the global as ... identifying newer markets and exploring the expansion of the ... Includes forecast from 2015 to 2020. Use ... the expansion of the present application market for various ... technology, determine its current market size, and estimate the ...
(Date:1/6/2016)... 2016  Varam Capital, a provider of micro-finance inclusion ... deliver advanced authentication solutions to their clients. Varam supplies ... A loan of a few thousand rupees may make ... ability to purchase livestock or equipment for a small ... for a local store. ...
Breaking Biology News(10 mins):
(Date:2/3/2016)... 2016  Discovery Laboratories, Inc. (NASDAQ: DSCO ... KL4 surfactant therapies for respiratory diseases, today announced ... inducement award as a component of employment compensation ... President and Chief Executive Officer.  The award was ... 1, 2016 and granted as an inducement material ...
(Date:2/3/2016)... Ascendis Pharma A/S (Nasdaq: ASND ), ... TransCon technology to address significant unmet medical needs, today ... Leerink Partners Global Healthcare Conference Location: , Waldorf ... 2016 Time:  , 11:55am EST www.ascendispharma.com ... An audio webcast of this event will be posted ...
(Date:2/3/2016)... , Feb. 3, 2016 New ... more than $1 million for researchers in ... on health-related research that demonstrates exciting potential.   ... of funding for the New Jersey Health Foundation Research ... members at these educational institutions— Princeton University, Rutgers ...
(Date:2/3/2016)... ... 2016 , ... ProMIS Neurosciences is currently in the process ... propagating strains of Amyloid beta involved in Alzheimer’s disease. The Company plans to ... on from the first misfolded Amyloid beta target announced on Nov. 12, 2015, ...
Breaking Biology Technology: