Navigation Links
Using combinatorial libraries to engineer genetic circuits advances synthetic biology
Date:4/22/2009

(Boston) -- Streamlining the construction of synthetic gene networks has led a team of Boston University researchers to develop a technique that couples libraries of diversified components with computer modeling to guide predictable gene network construction without the back and forth tweaking.

By applying engineering principles to biological systems where a set of components can evolve into networks that display desired behaviors known as synthetic biology -- , has led to new opportunities for biofabrication, drug manufacturing -- even potential biofuels.

And while there have been notable successes, the basic process of building and assembling a predictable gene network from bio-molecular parts remains a major challenge that is often frustrating. The time-consuming tweaking phase often requires many months of swapping out different chemical inputs, RNA regulators and promotors before the sought -after network is realized.

In a paper published online this week in Nature Biotechnology, the research team, led by James J. Collins, BU professor of biomedical engineering, focused on ways to speed up the construction process by assembling a library of 20 versions of two gene promotors and a simple synthesis technique to create component libraries for synthetic biology. Each version covered a wide range gene expression. With the activity levels calculated from the component libraries, the scientists turned to a computer model and designed and built a basic gene circuit to predict how fluorescent protein expression varied with levels of promoter-inhibiting chemicals.

Using the same simulation, for the simple gene circuit the researchers went the next step with a genetic timer, a more complicated circuit. However, computer simulation, on its own, was unable to predict the behavior of this timing circuit. They then built a representative genetic timer using a promoter from each of their libraries and, over time,
'/>"/>

Contact: Ronald Rosenberg
ronrosen@bu.edu
617-358-1240
Boston University
Source:Eurekalert

Page: 1 2

Related biology news :

1. K-State using Second Life island to help high school students learn earth science
2. Caltech scientists control complex nucleation processes using DNA origami seeds
3. VeriLook Surveillance SDK Provides Real-Time Face Identification Using Video Surveillance Cameras
4. VeriLook Surveillance SDK Provides Real-Time Face Identification Using Video Surveillance Cameras
5. Using hair to manage HIV/AIDS and predict treatment success
6. Milestone achieved toward production of malaria treatment using synthetic biology and fermentation
7. MIT: Using touch to help deaf people
8. Dana-Farber oncologists present at ASCO GU -- predict prostate cancer survival using Source MDx test
9. Biological control of tropical weeds using arthropods
10. Defying the disaster: Researcher explores resilient housing
11. Link between unexploded munitions in oceans and cancer-causing toxins determined
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/24/2015)... , Mar. 24, 2015 Research and Markets ... the "Global Iris Recognition Market 2015-2019" report ... Global Iris Recognition market to grow at a CAGR ... This report covers the present scenario and the growth ... period 2015-2019. To calculate the market size, the report ...
(Date:3/23/2015)... , March 23, 2015 SoundView Technology Group ... NXT-ID,s (NASDAQ: NXTD ) Wocket smart wallet. SoundView was one ... feedback on their experience with the Wocket in multiple scenarios ... CVS, Whole Foods and other retailers, making both debit and ... Tuttle also says, "If the company meets their plans ...
(Date:3/20/2015)... DUBLIN , Mar. 20, 2015 Research ... announced the addition of the "India Sensors ... their offering. The sensor market is ... through 2020 Consumer electronics, automotive, industrial ... for sensors in the country. In addition, adoption ...
Breaking Biology News(10 mins):Global Iris Recognition Market 2015-2019 with 3M Cogent, Bioenable Technologies, Cross Match Technology, Iris ID Systems & Safran Dominating 2Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 2Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 3Technology Research Note Update from SoundView; Real-World Usage and Feedback of Wocket Smart Wallet at CVS, Whole Foods and other Retailers 4India Sensors Market Forecast and Opportunities 2020 - STMicroelectronics held the largest share in 2014 2India Sensors Market Forecast and Opportunities 2020 - STMicroelectronics held the largest share in 2014 3
... first time that a clear link has been made between ... risk of Autism Spectrum Disorder and follows from a study ... The research, led by The University of Manchester, also confirms ... weight babies appear more susceptible to the condition. , Autism ...
... that measures the expression levels of 58 genes in ... who are at higher and lower risk for having ... five years after diagnosis, researchers report. The ... women with these cancers is getting nearer, says study ...
... May 2013 -- An important new study has revealed ... variation influences gene expression profiles of breast cancer. ... expression measurements may benefit from pooling of biopsies from ... Breast Cancer Conference in Brussels, Belgium. These findings ...
Cached Biology News:Bigger birth weight babies at greater risk of autism 2Gene expression test distinguishes btw breast cancer patients at high & low risk of late recurrence 2Gene expression test distinguishes btw breast cancer patients at high & low risk of late recurrence 3Study reveals magnitude of variation in gene expression measurements within breast cancers 2Study reveals magnitude of variation in gene expression measurements within breast cancers 3
(Date:3/25/2015)... Having added two International locations in Brisbane and ... Global proudly announced recently the organization’s expansion into Poland. ... in the United States, is one of the largest ... real estate. , Saltwood International is the ... advises occupiers of office space, ensuring that its corporate ...
(Date:3/25/2015)... , March 25, 2015 /CNW/ - The Gairdner ... the 2015 Canada Gairdner Awards, recognizing some of the ... Among the world,s most esteemed ... in science and provide a $100,000 (CDN) prize to each ... stronger culture of research and innovation across the ...
(Date:3/25/2015)... -- CASI Pharmaceuticals, Inc. (Nasdaq: CASI ), a biopharmaceutical ... innovative therapeutics addressing cancer and other unmet medical needs ... China , announces that it has ... drug candidate ENMD-2076 in triple-negative breast cancer (TNBC) at ... in Beijing , China.  Binghe ...
(Date:3/25/2015)... DNA Spectrum , a leading consumer ... product earlier this year. This consumer offering is designed ... currently stands. It does so through analysis of customers’ ... affect physical fitness. Once this analysis is completed, myDNA ... effects with the client's body profile and the latest ...
Breaking Biology Technology:International Organization Expands its Global Reach to Warsaw, Poland 22015 Canada Gairdner Awards Honour Top Medical Discoveries Around The World 22015 Canada Gairdner Awards Honour Top Medical Discoveries Around The World 32015 Canada Gairdner Awards Honour Top Medical Discoveries Around The World 42015 Canada Gairdner Awards Honour Top Medical Discoveries Around The World 52015 Canada Gairdner Awards Honour Top Medical Discoveries Around The World 62015 Canada Gairdner Awards Honour Top Medical Discoveries Around The World 72015 Canada Gairdner Awards Honour Top Medical Discoveries Around The World 8CASI Pharmaceuticals Announces Initiation Of A Phase 2 Trial With ENMD-2076 In Triple-Negative Breast Cancer In China 2CASI Pharmaceuticals Announces Initiation Of A Phase 2 Trial With ENMD-2076 In Triple-Negative Breast Cancer In China 3CASI Pharmaceuticals Announces Initiation Of A Phase 2 Trial With ENMD-2076 In Triple-Negative Breast Cancer In China 4CASI Pharmaceuticals Announces Initiation Of A Phase 2 Trial With ENMD-2076 In Triple-Negative Breast Cancer In China 5DNA Spectrum Educates the Bay Area Fitness Community about myDNA Fitness at SCW California MANIA 2DNA Spectrum Educates the Bay Area Fitness Community about myDNA Fitness at SCW California MANIA 3DNA Spectrum Educates the Bay Area Fitness Community about myDNA Fitness at SCW California MANIA 4
... Fledgling biotech company, TFX Bioscience, proves that Wisconsin has what ... arena. , ,TFX Bioscience, headquartered in Madison, ... at the Michigan Growth Capital Symposium, held in Ann Arbor ... that offer an innovative technology and an engaging business plan. ...
... Computerworld has just released their top 100 Best ... Wisconsin companies have placed in the top 50, with ... , ,This years list, with Hershey Foods in ... working conditions and employment benefits. According to Ellen Fanning, ...
... which provides enterprise-wide solutions to maximize profits for ... Group, Inc., , a global provider of business ... offer its customers Vanguard's Graphical Performance Series technology ... Application. , ,The Vanguard GPS solution broadens ...
Cached Biology Technology:Madisons TFX a Rising Star 2Wisconsin Companies Ranked Top in Nation in IT 2Wisconsin Companies Ranked Top in Nation in IT 3Wisconsin Companies Ranked Top in Nation in IT 4WorkWise and Vanguard Announce Partnership 2
Donor Horse Serum US Origin...
Request Info...
Mouse Aminopeptidase P2/XPNPEP2 Biotinylated Aff Pur PAb Keywords: AminopeptidaseP2, Metalloproteinase, Metalloprotease, X-Prolyl Aminopeptidase 2, X-Pro Aminopeptidase 2, Membrane-bound Prote...
Request Info...
Biology Products: