Navigation Links
Using a mathematical model to evaluate microsatellite genotyping from low-quality DNA
Date:9/1/2011

Noninvasive sampling has been used in studies of endangered animals. It has the advantage of obtaining samples without affecting the target animals. However, the quality of DNA obtained by such methods is often poor and this can affect the reliability of the results. Therefore, how to obtain reliable results from samples obtained noninvasively is of widespread interest. Professor LI Baoguo and Guo Songtao at the Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, set out to tackle this problem. Their work, entitled "Evaluating the reliability of microsatellite genotyping from low-quality DNA templates with a polynomial distribution model", was published in the Chinese Science Bulletin.2011, Vol 56 (24).

DNA templates from noninvasive samples, museum specimens, ancient DNA and forensic samples are often degraded or contaminated. The amount of target DNA available for genetic typing can be very low, often in the picogram range. When such low quality DNA is used for microsatellite profiling, false homozygotes and false alleles can occur leading to spurious results. To reduce the chance of false results, a multiple-tube approach was developed in which the DNA was distributed among several tubes. Compared to a single tube approach, this multi-tube approach offers more reliable genotyping from diluted DNA samples. However, the use of multiple PCR replicates can result in the sample being completely used up, especially if many loci are analyzed. In general, the smaller the amount of template in the PCR, the more prone it is to genotyping errors and the more PCR replicates are required.

Evaluating the reliability of microsatellite genotyping using low quality DNA templates can help optimize the experimental protocol. In this study, we aim to address the following questions for a given batch of samples: (i) how many amplifications are required to reach a given co
'/>"/>

Contact: Guo SongTao
songtaoguo@nwu.edu.cn
86-298-830-2418
Science in China Press
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. NJIT professor finds engineering technique to identify disease-causing genes
2. Research shows that the pill does not deserve its reputation for causing weight gain
3. Planetary "first family" discovered by astronomers using Gemini and Keck Observatories
4. Planetary first family discovered by astronomers using Gemini and Keck Observatories
5. Using water to understand human society, from the industrial revolution to global trade
6. ESA satellites focusing on the Arctic
7. Using the other guys toolkit: Similarities of pumping blood, oil examined
8. Bangladesh Voter Registration Project Now Using MegaMatcher Biometric Technology to Detect and Prevent Duplicate Registrations
9. City of Newark first in nation using cars to power grid
10. Warmer climate causing huge increase in tree mortality across the West
11. Cancer-causing gene discovery suggests new therapies
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Using a mathematical model to evaluate microsatellite genotyping from low-quality DNA
(Date:10/22/2014)... identity analyst firm Acuity Market Intelligence forecasts that in ... have a chip-based National eID card, including near-complete regional ... Asia , with its vast population, is expected ... of all National eID cards issued between 2014 and ... National eID Industry Report: 2014 Edition" — projects ...
(Date:10/18/2014)... a group of 2,000 patients referred for evaluation ... potential molecular diagnosis for 25 percent, including detection ... new mutations contributing to disease, according to a ... is being released to coincide with the American ... sequencing analyzes the exons or coding regions of ...
(Date:10/17/2014)... for the first time how bacteria can grow directly ... the opportunity to get tremendous insights into bacteria behavior ... discovered the bacterial growth in chronic lung infections among ... by the immune cells. The researchers discovered the immune ... bacteria, forcing the bacteria to switch to a much ...
Breaking Biology News(10 mins):Half the World's Population Will Have Chip-Based National Electronic Identity (eID) Cards by 2018 2Whole-exome sequencing shows potential as diagnostic tool 2Scientists opens black box on bacterial growth in cystic fibrosis lung infection 2
... Panama Canal in a Boston Whaler may sound like the ... story brandishes a butterfly net and studies the effects of ... Institute. , "Our long-term study shows that El ... Robert Srygley, former Smithsonian post doctoral fellow who is now ...
... is available in Spanish . , Individuals ... nuts, whole grains and fish- appear less likely to develop ... published in the October issue of Archives of General ... been found to be lower in Mediterranean than Northern European ...
... 09, 2009) -- According to new research from the Monell ... herbicides and lipid-lowering fibrate drugs act in humans to block ... pancreas. Commonly used in agriculture and medicine, these ... T1R3 receptor. The T1R3 receptor is a critical ...
Cached Biology News:Panama butterfly migrations linked to El Nio, climate change 2Mediterranean diet associated with reduced risk of depression 2Common herbicides and fibrates block nutrient-sensing receptor found in gut and pancreas 2
(Date:10/22/2014)... Oct. 22, 2014 Research and ... "Global DNA Diagnostics Market (Product types, Application, ... Global Trends, Company Profiles, Demand, Insights, Analysis, Research, ... report to their offering. ... applications, techniques, end users and Geography) Global Size, ...
(Date:10/22/2014)... October 22, 2014 Involution Studios ... release of a new infographic, Understanding Ebola . ... a beautifully designed, easy to follow informative tool for ... of outbreak, symptoms and prevention. , "As the news ... the 2014 Ebola outbreak represents not only a healthcare ...
(Date:10/22/2014)... According to new market research ... (Consulting, Integration, Deployment & Support), by Service Model ... Private, Others (Hybrid & Community)) - Global Forecast ... the Cloud Professional Services Market into various sub-segments ... It also identifies the drivers and restraints for ...
(Date:10/22/2014)... KONG , Oct. 22, 2014  aTyr Pharma ... that rare disease expert John C. McKew , ... McKew brings more than two decades of expertise in ... Institutes of Health, Wyeth Research and Genetics Institute, Inc. ... aTyr,s efforts to expand and translate its novel Physiocrine ...
Breaking Biology Technology:Global DNA Diagnostics Market (Product types, Application, Technology, End User and Geography) - Forecast to 2020 2Global DNA Diagnostics Market (Product types, Application, Technology, End User and Geography) - Forecast to 2020 3Involution Studios' Ebola Infographic Provides Key Disease Information and Statistics at a Glance 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 4Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 5aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3
... State agencies have backed a new organization, ... with early-stage companies. , ,Wisconsin excels in researching and ... investment in the first and most critical stage, said ... formation on Tuesday. , ,The Wisconsin Technology Council ...
... made Computerworld magazines recently released list of ... , , Christopher A. Graham, vice president and CIO of ... provides insurance plans to religious organizations, including churches as well ... clients across all 50 states. , , Herman Nell, senior ...
... College recently finished the first semester of its ... will soon be taught at technical colleges all over ... for security specialists. , ,MATC, one of the largest ... degree development process down to five months. Network security, ...
Cached Biology Technology:Computerworld: Three Wisconsin CIOs among top 100 IT leaders 2MATCs new computer security program gears up for second semester 2MATCs new computer security program gears up for second semester 3
... siSolutions siRNA Custom Synthesis ... effective duplex RNA to knockdown ... 21-mers (19 RNA base pairs ... recently-published 27-mers, or other lengths ...
Alexa Fluor 647 anti-mouse Qa-2...
...
Alexa Fluor 647 anti-mouse Qa-2...
Biology Products: