Navigation Links
Untangling whole genomes of individual species from a microbial mix
Date:5/23/2014

BETHESDA, MD May 23, 2014 A new approach to studying microbes in the wild will allow scientists to sequence the genomes of individual species from complex mixtures. It marks a big advance for understanding the enormous diversity of microbial communities including the human microbiome. The work is described in an article published May 22 in Early Online form in the journal G3: Genes|Genomes|Genetics, published by the Genetics Society of America.

"This new method will allow us to discover many currently unknown microbial species that can't be grown in the lab, while simultaneously assembling their genome sequences," says co-author Maitreya Dunham, a biologist at the University of Washington's Department of Genome Sciences.

Microbial communities, whether sampled from the ocean floor or a human mouth, are made up of many different species living together. Standard methods for sequencing these communities combine the information from all the different types of microbes in the sample. The result is a hodgepodge of genes that is challenging to analyze, and unknown species in the sample are difficult to discover.

"Our approach tells us which sequence fragments in a mixed sample came from the same genome, allowing us to construct whole genome sequences for individual species in the mix," says co-author Jay Shendure, also of the University of Washington's Department of Genome Sciences.

The key advance was to combine standard approaches with a method that maps out which fragments of sequence were once near each other inside a cell. The cells in the sample are first treated with a chemical that links together DNA strands that are in close proximity. Only strands that are inside the same cell will be close enough to link. The DNA is then chopped into bits, and the linked portions are isolated and sequenced.

"This elegant method enables the study of microbes in the environment," says Brenda Andrews, editor-in-chief of the journal G3: Genes|Genomes|Genetics. Andrews is also Director of the Donnelly Centre and the Charles H. Best Chair of Medical Research at the University of Toronto. "It will open many windows into an otherwise invisible world."

At a time when personal microbiome sequencing is becoming extremely popular, this method breaks important ground in helping researchers to build a complete picture of the genomic content of complex mixtures of microorganisms. This complete picture will be crucial for understanding the impact of varying microbiome populations and the relevance of particular microorganisms for individual health.


'/>"/>
Contact: Raeka Aiyar
press@genetics-gsa.org
202-412-1120
Genetics Society of America
Source:Eurekalert  

Related biology news :

1. Untangling Alzheimers disease
2. Untangling lifes origins
3. Frozen meal eaters get more vegetables including greens, beans and whole grains but with lower total calories vs. fast food restaurant eaters
4. New method developed for ranking disease-causal mutations within whole genome sequences
5. Study suggests whole diet approach to lower CV risk has more evidence than low-fat diets
6. Whole-genome sequencing uncovers the mysteries of the endangered Chinese alligator
7. Whole genome sequencing of wild rice reveals the mechanisms underlying oryza genome evolution
8. Foods identified as whole grain not always healthy
9. Making whole wheat bread taste and smell more appetizing
10. New whole plant therapy shows promise as an effective and economical treatment for malaria
11. BGI Tech develops whole exome sequencing analysis of FFPE DNA samples to boost biomedicine
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Untangling whole genomes of individual species from a microbial mix
(Date:7/31/2020)... ... 30, 2020 , ... Justin Zamirowski to lead upcoming launches ... focus on Type 2 diabetes and associated comorbidities. , Justin brings over 20 ... classes. As Chief Commercial Officer, Justin will lead Better Therapeutics’ efforts ...
(Date:7/10/2020)... , ... July 08, 2020 , ... ... Cell and Gene Therapy Regulation, An FDAnews Webinar, Wednesday, July 22, 2020 • ... comparability study, but what is the most effective way to complete one? Will ...
(Date:7/2/2020)... ... July 02, 2020 , ... ... B.V. (MBS) has announced a publication detailing the use of its revolutionary ... polymerase chain reaction (RT-PCR) in 16 minutes. The article, titled "Ultra-fast one-step ...
Breaking Biology News(10 mins):
(Date:8/12/2020)... HOUSTON (PRWEB) , ... August ... ... a biopharmaceutical cold chain validation engineering firm, is making available for ... driving the need to incorporate concurrent transport simulation testing into today’s biologics ...
(Date:8/3/2020)... (PRWEB) , ... August 03, 2020 , ... Introducing Ardent ... for transformative growth, , Known as MediVet Biologics since its ... , The new Ardent Animal Health will build on its base of ...
(Date:7/31/2020)... ... July 29, 2020 , ... ... up to three different materials for use with CRM, neurostimulation, vascular, and related ... materials to meet specific design requirements, performance, and cost criteria; especially where solid ...
(Date:7/31/2020)... (PRWEB) , ... July 30, ... ... of cloud-based software for the life sciences industry, today announced the release ... platform designed specifically for Medical Science Liaisons (MSLs) and other field medical ...
Breaking Biology Technology: