Navigation Links
Untangling whole genomes of individual species from a microbial mix
Date:5/23/2014

BETHESDA, MD May 23, 2014 A new approach to studying microbes in the wild will allow scientists to sequence the genomes of individual species from complex mixtures. It marks a big advance for understanding the enormous diversity of microbial communities including the human microbiome. The work is described in an article published May 22 in Early Online form in the journal G3: Genes|Genomes|Genetics, published by the Genetics Society of America.

"This new method will allow us to discover many currently unknown microbial species that can't be grown in the lab, while simultaneously assembling their genome sequences," says co-author Maitreya Dunham, a biologist at the University of Washington's Department of Genome Sciences.

Microbial communities, whether sampled from the ocean floor or a human mouth, are made up of many different species living together. Standard methods for sequencing these communities combine the information from all the different types of microbes in the sample. The result is a hodgepodge of genes that is challenging to analyze, and unknown species in the sample are difficult to discover.

"Our approach tells us which sequence fragments in a mixed sample came from the same genome, allowing us to construct whole genome sequences for individual species in the mix," says co-author Jay Shendure, also of the University of Washington's Department of Genome Sciences.

The key advance was to combine standard approaches with a method that maps out which fragments of sequence were once near each other inside a cell. The cells in the sample are first treated with a chemical that links together DNA strands that are in close proximity. Only strands that are inside the same cell will be close enough to link. The DNA is then chopped into bits, and the linked portions are isolated and sequenced.

"This elegant method enables the study of microbes in the environment," says Brenda Andrews, editor-in-chief of the journal G3: Genes|Genomes|Genetics. Andrews is also Director of the Donnelly Centre and the Charles H. Best Chair of Medical Research at the University of Toronto. "It will open many windows into an otherwise invisible world."

At a time when personal microbiome sequencing is becoming extremely popular, this method breaks important ground in helping researchers to build a complete picture of the genomic content of complex mixtures of microorganisms. This complete picture will be crucial for understanding the impact of varying microbiome populations and the relevance of particular microorganisms for individual health.


'/>"/>
Contact: Raeka Aiyar
press@genetics-gsa.org
202-412-1120
Genetics Society of America
Source:Eurekalert  

Related biology news :

1. Untangling Alzheimers disease
2. Untangling lifes origins
3. Frozen meal eaters get more vegetables including greens, beans and whole grains but with lower total calories vs. fast food restaurant eaters
4. New method developed for ranking disease-causal mutations within whole genome sequences
5. Study suggests whole diet approach to lower CV risk has more evidence than low-fat diets
6. Whole-genome sequencing uncovers the mysteries of the endangered Chinese alligator
7. Whole genome sequencing of wild rice reveals the mechanisms underlying oryza genome evolution
8. Foods identified as whole grain not always healthy
9. Making whole wheat bread taste and smell more appetizing
10. New whole plant therapy shows promise as an effective and economical treatment for malaria
11. BGI Tech develops whole exome sequencing analysis of FFPE DNA samples to boost biomedicine
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Untangling whole genomes of individual species from a microbial mix
(Date:4/5/2017)... 5, 2017 Today HYPR Corp. , ... server component of the HYPR platform is officially ... end-to-end security architecture that empowers biometric authentication across Fortune ... already secured over 15 million users across the financial ... connected home product suites and physical access represent a ...
(Date:4/3/2017)... WASHINGTON , April 3, 2017 /PRNewswire-USNewswire/ ... single-cell precision engineering platform, detected a statistically ... cell product prior to treatment and objective ... highlight the potential to predict whether cancer ... prior to treatment, as well as to ...
(Date:3/30/2017)... , March 30, 2017 Trends, opportunities and ... and behavioral), by technology (fingerprint, AFIS, iris recognition, facial ... and others), by end use industry (government and law ... financial and banking, and others), and by region ( ... , Asia Pacific , and the ...
Breaking Biology News(10 mins):
(Date:4/28/2017)... ... April 28, 2017 , ... KCAS Bioanalytical and ... 0.2 ng/mL lower limit (LLOQ) assay for nicotine and two key metabolites cotinine ... ultra-low trace nicotine assay meets additional needs of tobacco industry that need to ...
(Date:4/27/2017)... MD (PRWEB) , ... April 27, 2017 , ... ... (CAI) and a winner of the 43North Startup Competition, has truly taken their ... Cygnus spacecraft delivered Oncolinx’s innovative cancer experiments from Cape Canaveral to the International ...
(Date:4/27/2017)... ... ... Mitotech S.A, a Luxembourg based clinical stage biotechnology company, announced positive results ... rare devastating genetic disease that leads to a sudden and rapid loss of central ... carrying 11778, 14484 and 3460 mutations and having experienced the onset of symptoms more ...
(Date:4/27/2017)... ... April 27, 2017 , ... Arrowhead Publishers is pleased to ... coming to San Diego, CA on September 27-28, 2017. Leaders from the pharmaceutical, ... advances in the treatment of various types of pain. There are also extended ...
Breaking Biology Technology: