Navigation Links
Untangling whole genomes of individual species from a microbial mix
Date:5/23/2014

BETHESDA, MD May 23, 2014 A new approach to studying microbes in the wild will allow scientists to sequence the genomes of individual species from complex mixtures. It marks a big advance for understanding the enormous diversity of microbial communities including the human microbiome. The work is described in an article published May 22 in Early Online form in the journal G3: Genes|Genomes|Genetics, published by the Genetics Society of America.

"This new method will allow us to discover many currently unknown microbial species that can't be grown in the lab, while simultaneously assembling their genome sequences," says co-author Maitreya Dunham, a biologist at the University of Washington's Department of Genome Sciences.

Microbial communities, whether sampled from the ocean floor or a human mouth, are made up of many different species living together. Standard methods for sequencing these communities combine the information from all the different types of microbes in the sample. The result is a hodgepodge of genes that is challenging to analyze, and unknown species in the sample are difficult to discover.

"Our approach tells us which sequence fragments in a mixed sample came from the same genome, allowing us to construct whole genome sequences for individual species in the mix," says co-author Jay Shendure, also of the University of Washington's Department of Genome Sciences.

The key advance was to combine standard approaches with a method that maps out which fragments of sequence were once near each other inside a cell. The cells in the sample are first treated with a chemical that links together DNA strands that are in close proximity. Only strands that are inside the same cell will be close enough to link. The DNA is then chopped into bits, and the linked portions are isolated and sequenced.

"This elegant method enables the study of microbes in the environment," says Brenda Andrews, editor-in-chief of the journal G3: Genes|Genomes|Genetics. Andrews is also Director of the Donnelly Centre and the Charles H. Best Chair of Medical Research at the University of Toronto. "It will open many windows into an otherwise invisible world."

At a time when personal microbiome sequencing is becoming extremely popular, this method breaks important ground in helping researchers to build a complete picture of the genomic content of complex mixtures of microorganisms. This complete picture will be crucial for understanding the impact of varying microbiome populations and the relevance of particular microorganisms for individual health.


'/>"/>
Contact: Raeka Aiyar
press@genetics-gsa.org
202-412-1120
Genetics Society of America
Source:Eurekalert  

Related biology news :

1. Untangling Alzheimers disease
2. Untangling lifes origins
3. Frozen meal eaters get more vegetables including greens, beans and whole grains but with lower total calories vs. fast food restaurant eaters
4. New method developed for ranking disease-causal mutations within whole genome sequences
5. Study suggests whole diet approach to lower CV risk has more evidence than low-fat diets
6. Whole-genome sequencing uncovers the mysteries of the endangered Chinese alligator
7. Whole genome sequencing of wild rice reveals the mechanisms underlying oryza genome evolution
8. Foods identified as whole grain not always healthy
9. Making whole wheat bread taste and smell more appetizing
10. New whole plant therapy shows promise as an effective and economical treatment for malaria
11. BGI Tech develops whole exome sequencing analysis of FFPE DNA samples to boost biomedicine
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Untangling whole genomes of individual species from a microbial mix
(Date:5/16/2017)... 16, 2017   Bridge Patient Portal , ... MD EMR Systems , an electronic medical ... GE, have established a partnership to build an ... the GE Centricity™ products, including Centricity Practice Solution ... These new integrations will allow healthcare delivery ...
(Date:4/19/2017)... , April 19, 2017 ... its vendor landscape is marked by the presence of ... is however held by five major players - 3M ... these companies accounted for nearly 61% of the global ... leading companies in the global military biometrics market boast ...
(Date:4/13/2017)... , April 13, 2017 UBM,s Advanced Design ... will feature emerging and evolving technology through its ... Summits will run alongside the expo portion of the ... panels and demonstrations focused on trending topics within 3D ... design and manufacturing event will take place June 13-15, 2017 ...
Breaking Biology News(10 mins):
(Date:6/23/2017)... ... June 23, 2017 , ... RURO, Inc., a leading LIMS, ... its rapidly growing Laboratory Information System. , LimitLIS® version 3 is includes new ... provide more customization options. Each of these has been “under the microscope” in ...
(Date:6/23/2017)... ... June 23, 2017 , ... The Academy of Model ... University Aviation Association (UAA), the unifying voice for collegiate aviation education, are launching ... teamwork, competition, and success through a STEM-based education platform. , Much like the ...
(Date:6/22/2017)... ... 2017 , ... Building on the success of the inaugural RAADfest last year, ... latest developments in radical life extension. RAADfest combines cutting edge science presented for a ... personal development, making it the largest most comprehensive and inclusive super longevity event in ...
(Date:6/22/2017)... Boston, MA (PRWEB) , ... June 22, 2017 ... ... Kingdom-based social media network RegMedNet has produced a Spotlight ... has featured scholarly reviews and perspectives by leading experts on the unique regulatory ...
Breaking Biology Technology: