Navigation Links
'Unnatural' chemical allows Salk researchers to watch protein action in brain cells
Date:7/7/2011

ucted in two stages.

The first stage was to see if Uaas could be incorporated into neural stem cells, without disrupting their process of differentiation, and if so, would the fluorescent tag they inserted be carried into neuronal cells created by the stem cells.

"Current methods for Uaa incorporation are not appropriate for stem cells, because the added genes are often lost before the stem cell has a chance to finish differentiation," Wang says. To solve that problem, the researchers developed a lentiviral-based gene delivery method to incorporate the Uaas into proteins expressed in neural stem cells. "The lentiviral gene therapy technique, which was pioneered by Dr. Inder Verma at Salk, can afford long-lasting expression through stem cell differentiation," Wang says.

The virus was used to deliver different components needed in the Uaas technology. These consisted of a synthetic transfer RNA (tRNA) that cells use to incorporate amino acids into a protein that is being built inside a cell. The second molecule is an enzymatic synthetase that can recognize the engineered tRNA, and load it with the third engineered molecule - an unnatural amino acid. These amino acids are chemically distinct from the 20 amino acids that naturally exist in the body; they can be engineered for different desirable properties, such as to fluoresce.

"Once you have a stem cell line stably incorporating Uaas, you can custom the Uaa to study stem cell biology," Wang says. "You also get the bonus of obtaining various mature cells with this ability through differentiation, such as neurons, which are difficult to insert Uaas in and expensive to procure in large amounts."

In the first set of experiments, the researchers found out that Uaas were successfully incorporated into neural stem cells, the incorporation lasted through the differentiation, and these cells then produced neurons carrying the fluorescent amino acid.

The second set of exper
'/>"/>

Contact: Andy Hoang
ahoang@salk.edu
619-861-5811
Salk Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Intelligent people have unnatural preferences and values that are novel in human evolution
2. Salt marshes: A natural and unnatural history
3. BD Biosciences Introduces Chemically Defined Cell Culture Media Supplement that Increases Biopharmaceutical Protein Production
4. Study shows climate change makes some chemicals more toxic to aquatic life
5. Sugar-based chemicals book chapter by NJIT professor takes ACS kudos
6. Native ants use chemical weapons to turn back invading Argentine ants
7. Engineered liver may shed light on effects of chemicals in the environment
8. Therapies to improve biochemical functions hold promise as treatments for autism
9. Caltech researchers build largest biochemical circuit out of small synthetic DNA molecules
10. Nobel Laureate to speak at American Chemical Society green chemistry conference
11. Study Shows Sutro Biopharmas Biochemical Protein Synthesis Technology Enables Rapid Production and Scale-Up of Biopharmaceuticals
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/22/2014)... not be good for the average steak, but it ... prepare to survive Alaska,s winter cold. , "Alaska wood ... a steak does in your freezer and the frog ... shape than the steak," said Don Larson, University of ... recent paper demonstrating that freeze tolerance in Alaska wood ...
(Date:7/22/2014)... for Health Policy and Economics have linked tighter ... rates for schizophrenic individuals. , The study comes ... health actually save money, when other costs are ... an extra approval step before tests or treatments ... called prior authorization is intended to encourage ...
(Date:7/22/2014)... Highly purified crystals that split light with uncanny ... optics and, potentially, computers that manipulate light instead ... techniques, such as etching them with a precise ... expensive. , Now, researchers at Princeton and Columbia ... allow scientists to customize and grow these specialized ...
Breaking Biology News(10 mins):Alaska frogs reach record lows in extreme temperature survival 2Alaska frogs reach record lows in extreme temperature survival 3Are state Medicaid policies sentencing people with mental illnesses to prison? 2Technique simplifies the creation of high-tech crystals 2Technique simplifies the creation of high-tech crystals 3
... invertebrates to a wide range of temperature and ... species could have colonised the ocean depths. ... various times during Earth,s history caused extinctions of ... (>4,000 m) depths. These extinctions were apparently followed ...
... This release is available in Spanish . , ... distributions of 100 crops; 640 species of crop diseases, pests, ... and neighboring countries. Downloadable maps and geographic information system (GIS) ... that relating major wheat production areas to concentrations of Russian ...
... new study led by a University of Florida researcher uses ... evidence some of the fish swim directly to targeted locations. ... to orient at large distances, with tiger sharks swimming in ... resource areas about 30 miles away, said lead author Yannis ...
Cached Biology News:Shallow-water shrimp tolerates deep-sea conditions 2USDA and Russian scientists develop high-tech crop map 2New UF study shows some sharks follow 'mental map' to navigate seas 2New UF study shows some sharks follow 'mental map' to navigate seas 3
(Date:7/22/2014)... July 22, 2014 Holomic is ... the debut of its fluorescent immunoassay reader, the ... (AACC) meeting in Chicago, July 28-31, 2014. Available ... versions, the HRDR-300 is a highly sensitive, low-cost ... analyze fluorescent lateral flow immunoassays with high sensitivity ...
(Date:7/22/2014)... (PRWEB) July 22, 2014 Biofeedback ... hyperactivity disorder (ADHD) is diagnosed, selecting medication to best ... error. Analyzing brain patterns by using electroencephalography (EEG) can ... and thus lower the risk of adverse drug events. ... and Biomarkers” in the current issue of the ...
(Date:7/22/2014)... July 22, 2014 Dr. Williams says, ... who have recognized that the greatest health care system ... too often needlessly deadly while trying to serve the ... quasi-experts seeking to transform an enormous system that none ... Williams, in his continuing series of Open Letters ( ...
(Date:7/21/2014)... (PRWEB) July 21, 2014 With ... simple programming, the new Cole-Parmer® Digital Gear ... is easy to operate for automated process applications. ... hands-free dispensing. The batch count function counts down ... or totalizer tracks amount dispensed across multiple cycles. ...
Breaking Biology Technology:Holomic to Debut HRDR-300 Fluorescent Immunoassay Reader at the AACC 2014 2Analyzing Brain Patterns with EEG Can Help Predict Effective Use of Medication 2Analyzing Brain Patterns with EEG Can Help Predict Effective Use of Medication 3Dr. Ira Williams Asks What’s Wrong With Jonathan Bush’s Book, Where Does It Hurt? 2New Easy-View Cole-Parmer Digital Gear Pump Drive Makes Performance Monitoring Effortless 2
... TomoTherapy, Inc . has sold three of its advanced ... centers, the company announced. , ,The H. Lee ... University of South Florida in Tampa, will install three of ... by August of this year. The purchase price was not ...
... their personal information stolen. What's worse is that many ... of birth, social security number, or credit card information ... proper steps to protect themselves, their businesses, or their ... credit card or bank account information, can steal thousands ...
... - The Wisconsin Legislature has passed a bill that ... to commercialize their discoveries, but two other bills endorsed ... the remainder of the 2005-07 legislative session. , ,On ... Senate enacted their respective versions of Senate Bill 338, ...
Cached Biology Technology:Leading cancer institute to install three TomoTherapy systems 2Cracking down on identity theft 2Legislature adopts one tech bill, leaves two others twisting 2Legislature adopts one tech bill, leaves two others twisting 3Legislature adopts one tech bill, leaves two others twisting 4
... The Model 1575 immunowash microplate washer, 100-240 ... that have flat-, U-, or V-bottom wells. ... vertical needle positions to an accuracy of ... and overflow washing. It can store up ...
... MULTI-FUN Incubator is a compact ... uses a microprocessor controller with a ... temperature and agitation speed. Air circulation ... the incubator. Temperature can be easily ...
... MULTI-FUN Incubator is a compact benchtop ... a microprocessor controller with a large ... and agitation speed. Air circulation inside ... incubator. Temperature can be easily calibrated ...
... Incubator is a compact benchtop and ... microprocessor controller with a large display ... agitation speed. Air circulation inside to ... Temperature can be easily calibrated from ...
Biology Products: