Navigation Links
'Unnatural' chemical allows Salk researchers to watch protein action in brain cells
Date:7/7/2011

ucted in two stages.

The first stage was to see if Uaas could be incorporated into neural stem cells, without disrupting their process of differentiation, and if so, would the fluorescent tag they inserted be carried into neuronal cells created by the stem cells.

"Current methods for Uaa incorporation are not appropriate for stem cells, because the added genes are often lost before the stem cell has a chance to finish differentiation," Wang says. To solve that problem, the researchers developed a lentiviral-based gene delivery method to incorporate the Uaas into proteins expressed in neural stem cells. "The lentiviral gene therapy technique, which was pioneered by Dr. Inder Verma at Salk, can afford long-lasting expression through stem cell differentiation," Wang says.

The virus was used to deliver different components needed in the Uaas technology. These consisted of a synthetic transfer RNA (tRNA) that cells use to incorporate amino acids into a protein that is being built inside a cell. The second molecule is an enzymatic synthetase that can recognize the engineered tRNA, and load it with the third engineered molecule - an unnatural amino acid. These amino acids are chemically distinct from the 20 amino acids that naturally exist in the body; they can be engineered for different desirable properties, such as to fluoresce.

"Once you have a stem cell line stably incorporating Uaas, you can custom the Uaa to study stem cell biology," Wang says. "You also get the bonus of obtaining various mature cells with this ability through differentiation, such as neurons, which are difficult to insert Uaas in and expensive to procure in large amounts."

In the first set of experiments, the researchers found out that Uaas were successfully incorporated into neural stem cells, the incorporation lasted through the differentiation, and these cells then produced neurons carrying the fluorescent amino acid.

The second set of exper
'/>"/>

Contact: Andy Hoang
ahoang@salk.edu
619-861-5811
Salk Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Intelligent people have unnatural preferences and values that are novel in human evolution
2. Salt marshes: A natural and unnatural history
3. BD Biosciences Introduces Chemically Defined Cell Culture Media Supplement that Increases Biopharmaceutical Protein Production
4. Study shows climate change makes some chemicals more toxic to aquatic life
5. Sugar-based chemicals book chapter by NJIT professor takes ACS kudos
6. Native ants use chemical weapons to turn back invading Argentine ants
7. Engineered liver may shed light on effects of chemicals in the environment
8. Therapies to improve biochemical functions hold promise as treatments for autism
9. Caltech researchers build largest biochemical circuit out of small synthetic DNA molecules
10. Nobel Laureate to speak at American Chemical Society green chemistry conference
11. Study Shows Sutro Biopharmas Biochemical Protein Synthesis Technology Enables Rapid Production and Scale-Up of Biopharmaceuticals
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/22/2015)... DETROIT , Jan. 13, 2015 Technology Showcase, Hall ... iris-based identity authentication solutions, today announced it will showcase its ... and developed by the Department of Energy,s Oak Ridge ... International Auto Show . EyeLock,s iris identity authentication technology is ...
(Date:1/22/2015)... and POINT ROBERTS, Washington , January 20, ... leading sectors including technology and tech stocks, releases video from ... master pickpocket and security consultant Apollo Robbins . ... talks about the Wocket™ biometric smart wallet, a product of ...
(Date:1/22/2015)... YORK , Jan. 22, 2015   EyeLock, Inc. , ... it has appointed Steve Gerber to the new ... will be responsible for leading development of mobile platforms and ... years of success and innovation in the semiconductor industry to ...
Breaking Biology News(10 mins):EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 2EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 3Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 2Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 3EyeLock Names Steve Gerber as Senior Vice President of Mobile and Wearables 2
... office this week. The most exciting new film features ... using ground-breaking technology. For the first time ... an immune cell becoming infected by a parasite and ... the body. Professor Wolfgang Weninger, head of ...
... "FORE"...Though they may not help improve a person,s golf ... the local country club in the near future, following a ... 17,000 golf courses in the United States, and approximately 70 ... Ray Semlitsch, Curators, Professor of Biology in the MU College ...
... harmless virus to insert a corrective gene into mouse ... have alleviated sickle cell disease pathology. In their studies, ... no difference from normal mice. Although the scientists caution ... technical obstacles, they believe that the new therapy will ...
Cached Biology News:Scientists film inner workings of the immune system 2Golf course: Playing fields, wildlife sanctuaries or both 2Gene therapy corrects sickle cell disease in laboratory study 2Gene therapy corrects sickle cell disease in laboratory study 3
(Date:2/26/2015)... 2015 DuPont Executive Vice President James ... research advancements and product launches across the Agriculture and ... Merrill Lynch 2015 Global Agriculture Conference. ... supplies the last two years, long-term demand for agricultural ... the last decade, when demand for corn and soybeans ...
(Date:2/26/2015)... 26, 2015 On behalf of biotech ... companies, Wilentz, Goldman & Spitzer, P.A. (Wilentz) ... lawsuit against Genewiz Inc. in excess of $10 million. ... GenScript’s trade secrets, and that it improperly hired one ... an order denying motions for judgment notwithstanding the verdict, ...
(Date:2/26/2015)... , Feb. 26, 2015 The healthcare landscape ... ever-changing arena, Frost & Sullivan will host its 20 ... Executive MindXchange , March 8 to 10, at the Hilton ... Calif. This event is a platform ... leaders to discuss industry disruptions and how to succeed in ...
(Date:2/26/2015)... The Pittcon 2015 Exposition, which ... N. Morial Convention Center in New Orleans, Louisiana, ... 19, 2015) displaying products and services used by ... labs. The Exposition will offer the latest innovations ... analytical chemistry; drug discovery; nanotechnology; life sciences to ...
Breaking Biology Technology:DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 2DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 3DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 4DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 5Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 2Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 3Embrace the Future of Medical Technologies with Frost & Sullivan 2Embrace the Future of Medical Technologies with Frost & Sullivan 3Pittcon 2015 Announces Exposition Highlights 2
... life sciences organizations simplifies, the creation, review ... NEW YORK, Feb. 5 Intrasphere ... services and solutions to,life sciences organizations, announced ... solution powered by Microsoft Office,SharePoint Server 2007 ...
... Feb. 5 /Xinhua-PRNewswire/ -- China-Biotics, Inc. (OTC,Bulletin ... Chinese,firm specializing in the manufacture, research, development, ... the third quarter,fiscal 2008 earnings conference call ... be held at 9 a.m. EST on ...
... Calif., Feb. 5 Neurobiological,Technologies, Inc. (NTI(R)) (Nasdaq: ... 2008 financial results, for the quarter ended December,31, 2007, ... call on,February 11, 2008., Hosting the call will ... Craig W. Carlson, Vice President and Chief Financial Officer.,The ...
Cached Biology Technology:Intrasphere Technologies Releases PharmaCM Submissions Management and Authoring Module Powered by Microsoft Office SharePoint Server 2007 2Intrasphere Technologies Releases PharmaCM Submissions Management and Authoring Module Powered by Microsoft Office SharePoint Server 2007 3China-Biotics, Inc. Announces Rescheduling of Conference Call to Discuss Third Quarter 2008 Results 2China-Biotics, Inc. Announces Rescheduling of Conference Call to Discuss Third Quarter 2008 Results 3
Human peripheral blood CD14+ monocytes, For immunohistochemistry (IHC) Cell Chip with Human Peripheral Blood Monocytes, B Cells, NK Cells and Stem Cells...
Plasmacytoid dendritic cells, BDCA-4+, For immunohistochemistry (IHC) Cell Chip with Human Umbilical Cord Blood Dendritic Cells...
...
Adjustable tilt angle 0 to 20 Adjustable speed of 3-25 RPM Load capacity: 2.7 kg (6 lbs.) Ambient temperature range 4C to 65C Low voltage power is supplied by wall mount transformer...
Biology Products: