Navigation Links
Universitat Autonoma de Barcelona researchers first to clone mice in Spain
Date:6/12/2009

Researchers at the Department of Cell Biology, Physiology and Immunology at Universitat Autnoma de Barcelona (UAB) are the first to have cloned mice in Spain. Cloe, Cleo and Clona are three female brown-coloured mice and were born respectively on 12 May, 3 June and 10 June. By means of nuclear transfer techniques, scientist collected mature oocytes, removed their chromosomes and substituted them for the nucleus of an adult somatic cell. The cloning of mice is part of a research being carried out to study new ways to improve the efficiency of the cloning process.

All three mice were or are being suckled with other non-clones and their growth parameters are within normal range, say researchers who were in charge of cloning the mice, Nuno Costa-Borges, Josep Santal and Elena Ibez from the Department of Cell Biology, Physiology and Immunology at UAB.

In order to clone the animals, researchers collected oocytes and surrounding cumulus cells from several female mice. The chromosomes were extracted from each of the oocytes and substituted with a cell from the cumulus by cytoplasm injection. Once the oocytes had been reconstructed, they were activated by simulating the stimuli occurring during fecundation so as to induce embryonic development. The cloned embryos were later transferred to receptor females.

The mice obtained by researchers at UAB, in addition to being the first of their species cloned in Spain, are the first animals to survive at birth and develop correctly. In 2003, Spanish scientists were able to clone a female Pyrenean mountain goat using a cell from the last animal of this species, which became extinct in 2000. The cloned animal however died 10 minutes after it was born due to a severe lung defect.

Increase in the efficiency of the cloning process

The cloning of the mice forms part of a research which scientists at UAB are carrying out to discover new ways of improving the efficiency of the cloning process. Nuno Costa-Borges, Josep Santal and Elena Ibez are studying whether the use of valproic acid could contribute to an increase in the success rate of nuclear transfer cloning, currently situated at approximately 1% for mice using standard procedures.

Valproic acid is an inhibitor of the enzyme histone deacetylase, located at the cell nucleus where the DNA is found. Research carried out until now has shown that histone deacetylase inhibitors seem to contribute to an increase in levels of gene expression, which would favour the reprogramming of the somatic cell nucleus transferred to the oocyte cytoplasm. Its use in nuclear transfer processes however is very recent. It was first used two years ago and research until now has focused on trichostatin, an inhibitor which has significantly improved the efficiency of mouse cloning, raising it to 5%.

Studies carried out by researchers at UAB can not only be applied to reproductive cloning of animal models; they can also be used for the reprogramming of cells for therapeutic aims.

Costa-Borges, Santal and Ibez are comparing three groups of cloned embryos in their research: valproic acid in the first group, trichostatin in the second and no inhibiting substance in the third group. The three mice in this case were cloned using the first (Cloe and Clona) and second (Cleo) inhibitors. In vitro experiments already pointed to improvements in the development of cloned embryos using inhibitors. However, scientists must wait until the end of the in vivo test period in July to obtain more conclusive data.


'/>"/>

Contact: Maria Jesus Delgado
MariaJesus.Delgado@uab.cat
34-935-814-049
Universitat Autonoma de Barcelona
Source:Eurekalert

Related biology news :

1. IRB Barcelona to coordinate two European projects on biomedicine
2. Researchers at IRB Barcelona produce more data on key genes in diabetes
3. Barcelona Declaration 2008: Challenges and Pathways to Earth Sustainability
4. Protein that triggers plant cell division revealed by researchers
5. Researchers create freestanding nanoparticle films without fillers
6. Researchers identify new risk factor gene for rheumatoid arthritis
7. Researchers shed light on trading behavior in animals -- and humans
8. University of Saskatchewan and Canadian Synchrotron researchers shed light on esophageal disease
9. Faculty of Translational Medicine boosts support for biomedical researchers
10. USC researchers present diabetes findings at American Diabetes Association Scientific Sessions
11. MSU researchers receive $400,000 in first wave of stimulus funding
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/28/2017)... , March 28, 2017 The ... Hardware (Camera, Monitors, Servers, Storage Devices), Software (Video Analytics, ... Region - Global Forecast to 2022", published by MarketsandMarkets, ... 2016 and is projected to reach USD 75.64 Billion ... and 2022. The base year considered for the study ...
(Date:3/24/2017)... 24, 2017 The Controller General of Immigration from ... Abdulla Algeen have received the prestigious international IAIR Award for the ... Continue Reading ... ... Controller Abdulla Algeen (small picture on the right) have received the IAIR ...
(Date:3/23/2017)... The report "Gesture Recognition and Touchless Sensing Market by Technology (Touch-based ... to 2022", published by MarketsandMarkets, the market is expected to be worth USD ... 2022. Continue Reading ... ... ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... and LAGUNA HILLS, Calif. , Oct. ... Cancer Research, London (ICR) and University ... SKY92, SkylineDx,s prognostic tool to risk-stratify patients with multiple myeloma ... MUK nine . The University of Leeds ... partly funded by Myeloma UK, and ICR will perform the ...
(Date:10/10/2017)... ... 2017 , ... San Diego-based team building and cooking events company, Lajollacooks4u, has ... The bold new look is part of a transformation to increase awareness, appeal to ... period. , It will also expand its service offering from its signature gourmet cooking ...
(Date:10/10/2017)... 10, 2017 International research firm Parks Associates announced ... at the TMA 2017 Annual Meeting , October 11 in ... residential home security market and how smart safety and security products impact ... Parks Associates: Smart Home ... "The residential security market has ...
(Date:10/9/2017)... DIEGO , Oct. 9, 2017  BioTech ... biological mechanism by which its ProCell stem cell ... critical limb ischemia.  The Company, demonstrated that treatment ... amount of limbs saved as compared to standard ... the molecule HGF resulted in reduction of therapeutic ...
Breaking Biology Technology: