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Understanding the RNAi Reagents Market Overlap with Drug Discovery and Therapeutic Development is Critical for Pharmaceutical Leaders
Date:5/14/2012

p>Various RNAi -based products are currently in clinical trials with no marketed products to date, and many of the related patents are still pending. This report describes those RNAi technologies, along with design and methods of manufacture of siRNA reagents. These include chemical synthesis by in-vitro transcription and use of plasmid or viral vectors. Other approaches to RNAi include DNA-directed RNAi (ddRNAi) that is used to produce dsRNA inside the cell, which is cleaved into siRNA by the action of Dicer, a specific type of RNAse III. MicroRNAs are derived by processing of short hairpins that can inhibit the mRNAs. Expressed interfering RNA (eiRNA) is used to express dsRNA intracellularly from DNA plasmids.

A summary for this report and a free sample of the full document are available at http://www.giiresearch.com/report/jai70930-rnai.html

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