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Ultrasound idea: Prototype NIST/CU bioreactor evaluates engineered tissue while creating it
Date:5/3/2012

natural stimuli needed for the cells to create matrix proteins and develop the structure and properties of real cartilage. Ultrasound techniques monitor tissue changes over time, while a digital video microscope takes images.

Preliminary studies indicate the bioreactor both stimulates and monitors development of cells, matrix content and scaffolds to make three-dimensional engineered cartilage. The cell-laden gels were stimulated twice daily for an hour. Sulfated glycosaminoglycan (sGAG)which combines with fibrous proteins to form the extracellular matrixincreased significantly after seven days. This structural change was detected by a significant decrease in ultrasound signals after seven days.

The research described in the new paper was performed at and led by NIST. The bioreactor is a collaborative project with several co-authors from the University of Colorado Boulder (CU) Department of Chemical and Biological Engineering.

NIST and CU researchers continue to develop ultrasonic measurement methods and plan to conduct longer experiments. The bioreactor is also being used by other academic researchers as a tool for validating mathematical models of biokinetics, the study of growth and movement in developing tissue.


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Contact: Laura Ost
laura.ost@nist.gov
303-497-4880
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

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Ultrasound idea: Prototype NIST/CU bioreactor evaluates engineered tissue while creating it
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