Navigation Links
USC scientists generate first detailed map of human neuroreceptor
Date:9/11/2011

t to crystallize a necessary step for high-resolution imaging.

The biologist's usual go-to method to study such molecules growing a large quantity using molecular cloning failed to produce enough correctly structured α7 to study.

"You can't study it directly in its natural form, so you have to engineer it," Chen said.

In the case of α7, Chen's collaborator, Dr. Steve Sine from Mayo Clinic, engineered a chimera, a Frankenstein molecule sharing about 70 percent of its structure in common with the α7 that reacted to stimuli in the same way that natural α7 does.

The next step was to form crystals with these proteins for high-resolution study. This turns out to be particularly difficult for neuronal receptors because they are intrinsically flexible they need to bind to a neurotransmitter, a small molecule that acts as a messenger in the nervous system, and transmit the signal across the protein body. Moreover, these receptors are decorated with sugar molecules that add further flexibility to the system.

The crystallization of α7 was a painstaking process carried out by Shu-xing Li, the first author of the study and a postdoctoral fellow in Chen's lab. For every hundred crystals obtained, only one or two were good enough for structural analysis. Li had to sort through hundreds of crystals to collect enough data for structural analysis.

"In a sense, these crystals are probably among the world's most expensive crystals, certainly more expensive than diamond," Chen said. "But considering the rich information we can get from these crystals about human neuronal receptors, and the potential impact on drug development that can benefit human health, they are worth the effort."


'/>"/>

Contact: Robert Perkins
perkinsr@usc.edu
213-740-9226
University of Southern California
Source:Eurekalert

Page: 1 2

Related biology news :

1. Jefferson scientists deliver toxic genes to effectively kill pancreatic cancer cells
2. Scientists identify novel inhibitor of human microRNA
3. Argonne scientists peer into heart of compound that may detect chemical, biological weapons
4. MU scientists go green with gold, distribute environmentally friendly nanoparticles
5. Scientists identify gene that may contribute to improved rice yield
6. Scientists discover why a mothers high-fat diet contributes to obesity in her children
7. MU scientists see how HIV matures into an infection
8. Earth scientists keep an eye on Texas
9. Thinking it through: Scientists call for policy to guide biofuels industry toward sustainability
10. Scientists identify a molecule that coordinates the movement of cells
11. Scientists Find new migratory patterns for Mediterranean and Western Atlantic bluefin tuna
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/29/2014)... China — BGI Tech and Hebei Agricultural University jointly ... genome. Jujube is the most economically important member of ... difficult to sequence due the high level of heterozygosity ... that a genome in the Rhamnaceae (Buckthorn) family has ... Nature Communications . , Jujube is ...
(Date:10/29/2014)... new study on a large cohort of kidney cancer ... of the disease -- and reveals an apparent link ... cancer, particularly in Romania. , The research, by an ... and Genome Quebec Innovation Centre in Montreal, underscores the ... acid. The compound, found in plants of the Aristolochia ...
(Date:10/28/2014)... to expand the Office of Rare Diseases Research ... Sciences (ORDR-NCATS) collaborative Rare Diseases Clinical Research Network ... 22 consortia will work with 98 patient advocacy ... treatments for patients with rare diseases. The collaborations ... 2014 funding from NIH. , Rett syndrome, MECP2 ...
Breaking Biology News(10 mins):BGI Tech and Hebei Agricultural University complete the genome sequencing of the jujube tree 2Study sheds light on genetic architecture of kidney cancer 2Study sheds light on genetic architecture of kidney cancer 3NIH's ORDR-NCATS RDCRN & NICHD awards U54 cooperative agreement for natural history study 2NIH's ORDR-NCATS RDCRN & NICHD awards U54 cooperative agreement for natural history study 3
... sapiens migrating out of Africa began encountering Neanderthals, ... of thousands of years before. Despite their differences, ... produced children with genes from both lineages. Today, the ... remain alive in the genomes of humans with European ...
... a single gene in honey bees separates the queens from ... University and Wayne State University unraveled the gene,s inner workings ... Biology Letters . The gene, which is responsible for leg ... of bees, ability to carry pollen. "This gene is ...
... provider of scientific, technical and medical information products ... journal, Pathogenesis . Pathogenesis ... College of Pathologists a professional membership organization committed ... excellence in the practice of Pathology. The College ...
Cached Biology News:When populations collide 2When populations collide 3When populations collide 4Single gene separates queen from workers 2Elsevier announces the launch of open access journal: Pathogenesis 2
(Date:10/30/2014)... Investor-Edge has initiated coverage on ... Medivation Inc. (NASDAQ: MDVN ), Pharmacyclics Inc. ... MKT: SYN), and Ironwood Pharmaceuticals Inc. (NASDAQ: IRWD). Free ... http://investor-edge.com/register . On Wednesday, October 29, ... the Dow Jones Industrial Average declined 0.18%, to finish ...
(Date:10/30/2014)... SoundConnect , an industry leading ... end users the key to turning this already ... adding voice enablement and hybrid audio conferencing. , ... communication tool that includes presence, instant messaging and ... becomes a comprehensive unified communications solution. Adding the ...
(Date:10/30/2014)... Avure Technologies celebrates a year of success and ... its fleet of high volume High Pressure Processing (HPP) systems. ... return to PackExpo this year, which is where we first ... Jeff Williams, CEO at Avure. “Sales of the new Avure ... meeting the challenges of market demand for higher throughput. We ...
(Date:10/30/2014)... in the development of quantum technologies has been proposed ... published today (28 October) in Nature Communications , ... a new type of flexibly designed microscopic trap for ... and specialised superfast computers, often depend on harnessing the ... these tiny particles are hugely problematic because of the ...
Breaking Biology Technology:Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 2Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 3Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 4Biotech Equities Movers and Shakers - Organovo Holdings, Medivation, Pharmacyclics, Synthetic Biologics, and Ironwood Pharma 5SoundConnect Launches Voice Enablement and Hybrid Audio Conferencing for Office 365 2SoundConnect Launches Voice Enablement and Hybrid Audio Conferencing for Office 365 3In High Demand: New High Pressure Processing (HPP) System with Maximum Throughput Drives Strong Sales 2In High Demand: New High Pressure Processing (HPP) System with Maximum Throughput Drives Strong Sales 3
... DALLAS, October 14, 2010 ... ... --> ... var first_result; // Results are keyed by longUrl, so we need to grab the first one. for (var r ...
... Inc., a global leader in the research and development of ... its 2010 UCB Crohn,s and Me Scholarship Program ... 31 winners to receive a scholarship worth up to $10,000 ... The scholarship winners include Crohn,s disease patients, ...
... Kenya and Uganda have laid the groundwork for confined ... varieties of maize genetically modified to survive recurrent droughts ... is life, according to a speech given today by ... at the World Food Prize Symposium. Scientists working ...
Cached Biology Technology:The World Market for Diabetes Treatments Now Available at ReportsandReports 2The World Market for Diabetes Treatments Now Available at ReportsandReports 3The World Market for Diabetes Treatments Now Available at ReportsandReports 4The World Market for Diabetes Treatments Now Available at ReportsandReports 5The World Market for Diabetes Treatments Now Available at ReportsandReports 6The World Market for Diabetes Treatments Now Available at ReportsandReports 7The World Market for Diabetes Treatments Now Available at ReportsandReports 8The World Market for Diabetes Treatments Now Available at ReportsandReports 92010 UCB Crohn's and Me Scholarship Winners Announced 22010 UCB Crohn's and Me Scholarship Winners Announced 32010 UCB Crohn's and Me Scholarship Winners Announced 4Scientists prepare for confined field trials of life-saving drought-tolerant transgenic maize 2Scientists prepare for confined field trials of life-saving drought-tolerant transgenic maize 3
ERalpha (Ser 104/106)...
... Phosphatase, Calf Intestinal (CIAP), catalyzes the hydrolysis ... ribo- and deoxyribonucleoside triphosphates. This enzyme is ... linearized cloning vector DNA by removing phosphate ... be used for the dephosphorylation of 5 ...
Mouse CXCR3 Allophycocyanin MAb (Clone 220803)...
Homo sapiens palladin...
Biology Products: