Navigation Links
UMass Amherst researchers develop powerful new technique to study protein function
Date:6/19/2013

. elegans to explore how dopamine modulates the activities of specialized neurons. The worm is a useful model because it has only 302 neurons instead of billions in mammals. Despite its simplicity, the worm's basic neurotransmission mechanisms are also found in humans.

In the quest to identify genes that regulate dopamine signaling, the UMass Amherst researchers quickly recognized that dopamine acts through proteins used by other neurotransmitters in other nervous system cells. "So we couldn't use traditional genetic tools to study dopamine signaling. We needed to develop a new method to study protein function in individual cells in multicellular organisms," Chase notes.

The technique they developed takes advantage of nonsense-mediated decay (NMD), a surveillance mechanism present in all eukaryotic organisms. NMD destroys aberrant mRNA molecules that can arise naturally through mutation during transcription or mRNA processing.

"In our strategy, we replace the normal copy of a gene with a tagged version that targets the gene's mRNA for destruction by NMD," Chase explains. "We then remove NMD from all the organism's cells. Without NMD present, the replacement gene is expressed normally in all cells. We then knock down expression of the gene cell-specifically by restoring NMD activity only in cells we select."

He adds, "This cell-specific restoration of NMD activity is easy and can also be controlled in time. Thus, using NMD we can not only remove gene function in individual cell types, we can control exactly when gene function is removed in that cell type. This gives complete control of gene expression and allows one to investigate the function of any gene in any cell type at any time."

"With this very powerful new technique, now you can identify an individual gene and you can ask whether it plays a role in the behavior of interest. All these genes are expressed in our brains, so we are learning about all sorts of fa
'/>"/>

Contact: Janet Lathrop
jlathrop@admin.umass.edu
413-545-0444
University of Massachusetts at Amherst
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. UMass Amherst researchers reveal mechanism of novel biological electron transfer
2. UMass Medical School faculty elected to the American Association for the Advancement of Science
3. UMass Amherst biochemists developing tools to stop plague and other bacterial threats
4. UMass Medical School researchers discover a new role for RNAi
5. UMass Medical School scientist named 2012 Pew Latin-American Fellow in the Biomedical Sciences
6. UMass Amherst wildlife researcher photographs rare Sumatran rabbit
7. Chemical engineers at UMass Amherst find high-yield method of making xylene from biomass
8. NIST/UMass study finds evidence nanoparticles may increase plant DNA damage
9. Alnylam and UMass Medical School announce Tuschl I patent upheld in European opposition proceedings
10. Saint Louis University researchers discover a way to detect new viruses
11. OU researchers collaborate on $20 million NSF EPSCoR grant
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/21/2014)... 19, 2014  Earlier this year Donald Spector ... and one of the most prolific inventors in the ... transmitted from Smartphones to third party agencies. Spector envisioned ... one of the earliest known patents in this area. ... the military, child care, elder care and hospital applications. ...
(Date:11/21/2014)...   Atmel® Corporation (NASDAQ: ATML ... technology solutions, today launched the industry,s first family of ... V cc range from 1.7V to 5.5V. The ... 2 C bus communication speeds, and are available with ... ideal for consumer, industrial, computer, and medical applications. ...
(Date:11/18/2014)... , Nov. 17, 2014 The Parenteral Drug Association ... regulatory agencies will speak and at least seven more will ... at the Omni Shoreham Hotel in Washington D.C. ... again to have significant support from the regulatory agencies in ... Europe in our effort to help advance the ...
Breaking Biology News(10 mins):Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 2Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 3Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 4FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2
... at the Universities of Bristol, Florence, and Western Australia, might ... with the disorder. The faces we see in the ... points in a face-space, said Elizabeth Pellicano of the University ... average, or most typical, face, with more distinctive faces lying ...
... by investigators at St. Jude Childrens Research Hospital provide ... or Gleevec, which has revolutionized the treatment of chronic ... of a particularly aggressive form of acute lymphoblastic leukemia ... activity of a specific molecule, a strategy aimed at ...
... It appears that chemical warfare has been around a ... about 100 million years, give or take a little. ... has identified a soldier beetle, preserved almost perfectly in amber, ... fight off an attacker when an oozing flow of sap ...
Cached Biology News:Children with autism don't adapt as readily to unfamiliar faces 2St. Jude finds factors that accelerate resistance to targeted therapy in lymphoblastic leukemia 2Amber specimen captures ancient chemical battle 2Amber specimen captures ancient chemical battle 3
(Date:11/26/2014)... Whitehouse Laboratories is pleased to ... County (New Jersey) Chamber of Commerce as a ... organization in the North Jersey area, offers Whitehouse ... key clients in the pharmaceutical and biotechnology areas ... Membership enables Whitehouse Laboratories a higher level of ...
(Date:11/26/2014)... 25, 2014 Continuing its award-winning streak, ... three prestigious MarCom Awards for outstanding creative achievement by ... Platinum MarCom Award, the organization’s top honor, in the ... Louis held in April of this year. The event ... attendees. Additionally, Nerium was awarded a Gold MarCom Award ...
(Date:11/26/2014)... Miles Holder, formerly of Micron Research ... Products team as Sales/Marketing Manager. Mr. Holder has over ... for the graphite industry. , “We are thrilled to ... Dave Trinkley, VP of Market and Product Development for ... in the graphite industry, along with his proven track ...
(Date:11/26/2014)... Texas (PRWEB) November 26, 2014 ... Industry is an in-depth research report on the ... the report introduces Palmitic Acid, basic information, including ... policy analysis, and news analysis, etc. , ... analyzes Palmitic Acid market in China ...
Breaking Biology Technology:Whitehouse Laboratories Joins Morris County Chamber of Commerce 2Whitehouse Laboratories Joins Morris County Chamber of Commerce 3Nerium International™ Wins One Platinum, Two Gold MarCom Awards 2Miles Holder Joins Graphel Corporation as Sales/Marketing Manager 2Palmitic Acid Industry Global 2014 Analysis & 2020 Forecasts on Market data, Statics, Development Now at ReportsnReports.com 2Palmitic Acid Industry Global 2014 Analysis & 2020 Forecasts on Market data, Statics, Development Now at ReportsnReports.com 3
... Product Suite and Addresses Sample Preparation Bottlenecks -HOPKINTON, ... (Nasdaq: CALP ), a leading provider ... life sciences research, today launched the Zephyr Genomics ... for molecular biology applications. The inclusion of pre-installed ...
... Double Helix Consulting US (DHC US), headed by ... of highly experienced strategic pricing, reimbursement and market ... industry as well as strong commercial, governmental and ... to clients in the pharmaceutical, biotechnology and medical ...
... 26 /PRNewswire-Asia/ -- Sinovac Biotech Ltd.,(NYSE Alternext ... in China, today,announced its wholly owned subsidiary, ... approval from China,s Ministry of Agriculture to,conduct ... animal rabies,vaccine with independent intellectual property rights. ...
Cached Biology Technology:Caliper Life Sciences Introduces Zephyr Genomics Workstation for Automated Liquid Handling 2Caliper Life Sciences Introduces Zephyr Genomics Workstation for Automated Liquid Handling 3London-Based Double Helix Consulting Launches US Division for Strategic Pricing, Reimbursement and Market Access Solutions 2Sinovac Enters Veterinary Vaccine Market with Inactivated Rabies Vaccine 2Sinovac Enters Veterinary Vaccine Market with Inactivated Rabies Vaccine 3Sinovac Enters Veterinary Vaccine Market with Inactivated Rabies Vaccine 4
... Since the Flp-In System creates isogenic ... equivalent levels of the protein of ... in Figure 3. In this experiment ... (CAT) was subcloned into pcDNA5/FRT and ...
For the quantitative determination of cyclic AMP (cAMP) concentrations in cell culture supernates, serum, plasma, saliva, urine and cell lysates....
SHEEP ANTI BOVINE LACTOPEROXIDASE...
Fast, sensitive quantitation of total MEK 1/2 concentration....
Biology Products: