UI and ISU establish shared DNA sequencing instr...(ina is a short-read instrument that ca...)

Date:7/24/2008

ina is a "short-read" instrument that can sequence relatively short DNA fragments of 18 to 36 bases. The instrument is capable of sequencing many more fragments per run, producing in excess of a billion bases of sequence data, compared to the long-read machine, which generates around 100 million base pairs of sequence. The Illumina sequencer is well-suited for genome-wide applications, studies that analyze mutations and sequence variations associated with genetic diseases in humans and beneficial crop traits in plants. It includes ChIP-seq, digital gene expression tag profiling, whole genome and candidate gene re-sequencing, and small RNA identification and profiling.

"Sharing the two instruments will allow researchers at both universities to conduct research projects that might not have been economically feasible without this dual access," Polking said. "University of Iowa researchers who send DNA or RNA samples to our facility in Ames will pay the same rates as Iowa State researchers. We have been contacted by several UI and Iowa State researchers who are very excited about having access to both instruments."

"It doesn't matter that each institution only has one machine on site because this will function as one single facility," Apicella said. "Researchers from either institution now have access to two machines, which have different capabilities and will be useful for different projects. We really are sharing resources at every level."

Apicella added that additional infrastructure already is in place to take advantage of the sequencing power provided by the machines, including bioinformatics expertise and fiber optic cables between the two institutions to transfer the huge amounts of data generated by the machines.

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Contact: Stephen Howell shh@iastate.edu 515-294-5252 Iowa State University Source:Eurekalert

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