Navigation Links
UF researchers develop improved gene therapy agent
Date:5/19/2008

rt of its makeup a group of molecules called a hydroxyl group, which attracts phosphates in the cell. When a phosphate binds to the hydroxyl group, it sends a signal to the proteasome the cellular equivalent of taking the trash out to the curb.

So Srivastava and his UF College of Medicine colleagues decided to test what would happen if they took the phosphate out of the equation.

To do that, the researchers replaced tyrosine with another amino acid, phenylalanine. The two amino acids are identical except for one thing phenylalanine lacks the part that attracts phosphate.

We didnt change anything except the amino acid that does not allow phosphorylation to occur, he said. It was very simple. You can buy a kit from a company and can mutate any amino acid you want.

Tyrosine is found at seven spots on the surface of AAV, Srivastava said. The scientists created seven new vectors, replacing a different tyrosine in each one and inserting in them the gene that triggers production of the blood-clotting protein Factor IX. Patients with hemophilia B, a common form of the disease, do not naturally produce this protein.

In tissue samples and in mice, all the new vectors worked better than a commonly used version of AAV. One of the versions in particular worked 11 times better in tissue samples after 48 hours. In mice, the results were staggering. Two weeks after the mice were injected with the corrective gene, one of the new AAV-gene combos was working 29 times better than the standard vector was at incorporating the new gene into cells, at a 10-fold lower dose.

We were very surprised, Srivastava said. Its amazing to think that changing one amino acid could produce these results.

Now the virus actually completely avoids being phosphorylated, so it doesnt become degraded and it goes into the nucleus, and we get therapeutic levels of proteins. We can generate therapeutic levels of Factor IX.

The researche
'/>"/>

Contact: April Frawley Birdwell
afrawley@ufl.edu
352-273-5817
University of Florida
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Researchers document rapid, dramatic reverse evolution in the threespine stickleback fish
2. Stowers Institute researchers identify gene linked to vertebral defects in patient populations
3. Researchers discover architecture for fundamental processes of life
4. ASU researchers synthesize molecule with self-control
5. Researchers uncover mechanism of action of antibiotic able to reduce neuronal cell death in brain
6. Berkeley researchers identify photosynthetic dimmer switch
7. Magnet Lab researchers make observing cell functions easier
8. Researchers find way to make tumor cells easier to destroy
9. UC Davis stem researchers demonstrate safety of gene therapy using adult stem cells
10. Researchers report the cloning of a key group of human genes, the protein kinases
11. UT researchers find link between advertising and increased tobacco use among Indias youth
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/10/2014)... on U.S. store shelves in early 2010, and people ... small packets can be tossed into a washing machine ... powder. The convenience, though, has come with risks for ... Nationwide Children,s Hospital found that from 2012 through 2013, ... younger than 6 years of age swallowing, inhaling, or ...
(Date:11/6/2014)... paper from a team of Florida State University biologists ... could adapt to and survive environmental swings such as ... latest issue of the journal The Plant Cell ... DNA and proteins) is organized in a cell and ... are turned on and others are turned off. , ...
(Date:11/5/2014)... Santamaria, associate professor of biology in the UTSA ... in the nation selected to receive a two-year ... for Exploratory Research (EAGER). The funding supports President ... researchers to create new technology that will demystify ... complex behaviors in neuroscience are broken into the ...
Breaking Biology News(10 mins):Study finds laundry detergent pods, serious poisoning risk for children 2Maize analysis yields whole new world of genetic science 2UTSA biology professor awarded $300,000 NSF grant for brain research 2UTSA biology professor awarded $300,000 NSF grant for brain research 3
... published an overview of sales of various drugs used ... are shown in kilograms of active ingredient; antibacterials, anaesthetics, ... figures are based on sales from wholesalers to pharmacies ... an increase in sales of antibacterials from 2002-2006, sales ...
... the School of Biological Sciences at Queens University, found the ... University College Cork and BirdWatch Ireland. Its natural range is ... the closest it has been spotted to Ireland is in ... was studying the diet of the Barn Owl in Ireland. ...
... emerged as an important signaling molecule in plants - ... tropical medicinal herb as a model plant, researchers have ... and enzymes in plants. In collaborative work with ... and her doctoral student at the Botany Department, University ...
Cached Biology News:For good or ill Ireland gains another mammal species 2Nitric oxide regulates plants as well as people 2
(Date:11/26/2014)... Texas (PRWEB) November 26, 2014 ... Industry is an in-depth research report on the ... the report introduces Palmitic Acid, basic information, including ... policy analysis, and news analysis, etc. , ... analyzes Palmitic Acid market in China ...
(Date:11/24/2014)... Nov. 24, 2014  IGI Laboratories, Inc. (NYSE MKT: ... specialty generic pharmaceutical company, today announced it has submitted ... U.S. Food and Drug Administration (FDA), which brings the ... seven, with eighteen submissions now pending at the FDA. ... CEO of the Company, commented, "Our team here at ...
(Date:11/24/2014)... , Nov. 24, 2014   Veracyte, Inc. ... H. Anderson , president and chief executive officer, will present ... th Annual Healthcare Conference on Tuesday, December 2, 2014 ... . The live audio webcast and subsequent replay ... . Please connect to the website at least 15 minutes ...
(Date:11/24/2014)... , Nov. 24, 2014  Spherix Incorporated ... company committed to the fostering and monetization of ... v. VTech Telecommunications Ltd.,  Case No. 3:13-cv-03494-M and  ... No. 3:13-cv-03496-M, both in the United States District ... Texas . On November ...
Breaking Biology Technology:Palmitic Acid Industry Global 2014 Analysis & 2020 Forecasts on Market data, Statics, Development Now at ReportsnReports.com 2Palmitic Acid Industry Global 2014 Analysis & 2020 Forecasts on Market data, Statics, Development Now at ReportsnReports.com 3IGI Laboratories, Inc. Announces ANDA Submission 2Veracyte to Present at the Piper Jaffray 26th Annual Healthcare Conference 2Markman Hearing Held as Scheduled in VTech and Uniden Cases 2
... Data , , THOUSAND OAKS, Calif., June 22 ... that the U.S. Food and Drug Administration (FDA) has asked ... Health Drugs Advisory Committee (RHDAC) on Aug. 13, 2009. The ... for denosumab, a RANK Ligand inhibitor for which Amgen is ...
... Pharmaceutical Research and Manufacturers of America ... statement regarding today,s AARP-White House press conference on health care ... we believe this agreement will be looked back in time ... our troubled health care system. Our $80 billion pledge ...
... COLUMBUS, Ohio, June 22 Mettler-Toledo International Inc. (NYSE: ... has been appointed to its Board of Directors, effective July ... and Chief Executive Officer of Millipore Corporation (NYSE: ... research and biopharmaceutical manufacturing industry. Prior to joining Millipore ...
Cached Biology Technology:Amgen to Participate in Reproductive Health Drugs Advisory Committee Meeting in August 2Amgen to Participate in Reproductive Health Drugs Advisory Committee Meeting in August 3Amgen to Participate in Reproductive Health Drugs Advisory Committee Meeting in August 4Amgen to Participate in Reproductive Health Drugs Advisory Committee Meeting in August 5Amgen to Participate in Reproductive Health Drugs Advisory Committee Meeting in August 6Amgen to Participate in Reproductive Health Drugs Advisory Committee Meeting in August 7PhRMA Statement on AARP-White House Press Conference 2Martin Madaus Joins Mettler Toledo Board of Directors 2
MHC. Sections include: the isolation and functional properties of antigen-presenting cells analysis of MHC class II endocytosis and peptide turnover analysis of signalling via MHC class II molecu...
Supplemental reagent for annexin V assay....
Large-fragment, single-stranded carrier DNA of the highest quality, perfectly suited for all types of yeast transformation....
Qa-1 (L-12)...
Biology Products: