Navigation Links
UF researchers develop improved gene therapy agent
Date:5/19/2008

rt of its makeup a group of molecules called a hydroxyl group, which attracts phosphates in the cell. When a phosphate binds to the hydroxyl group, it sends a signal to the proteasome the cellular equivalent of taking the trash out to the curb.

So Srivastava and his UF College of Medicine colleagues decided to test what would happen if they took the phosphate out of the equation.

To do that, the researchers replaced tyrosine with another amino acid, phenylalanine. The two amino acids are identical except for one thing phenylalanine lacks the part that attracts phosphate.

We didnt change anything except the amino acid that does not allow phosphorylation to occur, he said. It was very simple. You can buy a kit from a company and can mutate any amino acid you want.

Tyrosine is found at seven spots on the surface of AAV, Srivastava said. The scientists created seven new vectors, replacing a different tyrosine in each one and inserting in them the gene that triggers production of the blood-clotting protein Factor IX. Patients with hemophilia B, a common form of the disease, do not naturally produce this protein.

In tissue samples and in mice, all the new vectors worked better than a commonly used version of AAV. One of the versions in particular worked 11 times better in tissue samples after 48 hours. In mice, the results were staggering. Two weeks after the mice were injected with the corrective gene, one of the new AAV-gene combos was working 29 times better than the standard vector was at incorporating the new gene into cells, at a 10-fold lower dose.

We were very surprised, Srivastava said. Its amazing to think that changing one amino acid could produce these results.

Now the virus actually completely avoids being phosphorylated, so it doesnt become degraded and it goes into the nucleus, and we get therapeutic levels of proteins. We can generate therapeutic levels of Factor IX.

The researche
'/>"/>

Contact: April Frawley Birdwell
afrawley@ufl.edu
352-273-5817
University of Florida
Source:Eurekalert

0

GOOD

Page: 1 2 3

Related biology news :

1. Researchers document rapid, dramatic reverse evolution in the threespine stickleback fish
2. Stowers Institute researchers identify gene linked to vertebral defects in patient populations
3. Researchers discover architecture for fundamental processes of life
4. ASU researchers synthesize molecule with self-control
5. Researchers uncover mechanism of action of antibiotic able to reduce neuronal cell death in brain
6. Berkeley researchers identify photosynthetic dimmer switch
7. Magnet Lab researchers make observing cell functions easier
8. Researchers find way to make tumor cells easier to destroy
9. UC Davis stem researchers demonstrate safety of gene therapy using adult stem cells
10. Researchers report the cloning of a key group of human genes, the protein kinases
11. UT researchers find link between advertising and increased tobacco use among Indias youth
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/21/2013)... ANN ARBORThe new University of Michigan Water Center ... to support Great Lakes restoration and protection efforts. ... will support diverse projects, including efforts to track ... effectiveness of techniques to control non-native weedy plant ... and monitor fish responses to restoration activities. , ...
(Date:5/21/2013)... scientists have developed many devices that can reopen clogged ... effective, each of these treatments has drawbacks, including the ... MIT analyzes the potential usefulness of a new treatment ... stents, but may pose fewer risks. With this new ... only a brief period, during which it releases a ...
(Date:5/20/2013)... are also commonly resistant to antimicrobial substances made ... in mBio , the online open-access journal ... colistin and host antimicrobials LL-37 and lysozyme, which ... mean that patients with life-threatening multi-drug resistant infections ... Colistin is a last-line drug for treating several ...
Breaking Biology News(10 mins):U-M Water Center awards $570K in Great Lakes restoration grants 2U-M Water Center awards $570K in Great Lakes restoration grants 3U-M Water Center awards $570K in Great Lakes restoration grants 4Evaluating a new way to open clogged arteries 2Evaluating a new way to open clogged arteries 3Resistance to last-line antibiotic makes bacteria resistant to immune system 2Resistance to last-line antibiotic makes bacteria resistant to immune system 3
... Montral, June 23, 2009 On June 18, the ... $9.16 million for the creation of a national technology ... platform, headed by Dr. Benoit Coulombe from the Institut ... provide Canadian researchers with new state-of-the-art equipment in proteomics, ...
... 2009) University of Minnesota Medical School researchers have discovered an ... to treat those infected by a parasitic flatworm. ... The Schistosomasis parasite infects about 200 million people in ... where people become infected simply by bathing, drinking, or cooking water ...
... whips up more powerful and frequent hurricanes and storms, the ... breed and recover from damage. That,s one of the ... corals in the wake of large climate-driven bleaching and storm ... the regrowth of young corals drops sharply in ...
Cached Biology News:A Canada-wide technology platform for mapping the human interactome 2U of M study finds new insight on therapy for a devastating parasitic disease 2Coral face 'a stormy future' 2
(Date:5/22/2013)... NEW YORK , May 22, 2013 /PRNewswire/ ... pioneering the development of disease modifying therapeutics for ... today the completion of its Series B financing, ... shares and warrants that would represent approximately $2.8M ... investors and new investors supported the raise. ...
(Date:5/22/2013)... N.H. , May 22, 2013  Superior ... the Year 2013" by Business NH Magazine.  For ... Granite State organizations that distinguish themselves with extraordinary ... very proud to receive this honor," said ... Controls.  "As with any recognition, this award is ...
(Date:5/22/2013)... (PRWEB) May 22, 2013 Genedata, ... industrial biotechnology, drug discovery, and life science research, ... Selector ™ as its bioinformatics platform for ... organisms. Genedata will provide AB Enzymes, one of ... a hosted solution of Genedata Selector accompanied by ...
(Date:5/22/2013)... 22, 2013 AudioNotch ... for tinnitus treatment. AudioNotch is pleased to announce ... clinic locations in both British Columbia and Alberta, ... and hearing services. AudioNotch's tinnitus treatment program is ... entering a Destination Hearing clinic, patients have the ...
Breaking Biology Technology:Oligomerix, Inc. Completes Series B Financing 2Oligomerix, Inc. Completes Series B Financing 3Superior Controls of Seabrook, NH named Business of the Year for 2013 by Business NH Magazine 2AB Enzymes Chooses Genedata Selector for Production Strain Development and Optimization 2AB Enzymes Chooses Genedata Selector for Production Strain Development and Optimization 3AB Enzymes Chooses Genedata Selector for Production Strain Development and Optimization 4AudioNotch Tinnitus Treatment Launches Partnership With Destination Hearing 2
... on QuatRx clinical development programs, including review of positive results ... ... ANN ARBOR, Mich., Jan. 4 QuatRx Pharmaceuticals Company,today announced that ... presenter at the 26th Annual JP Morgan Healthcare,Conference on Tuesday, January ...
... the Company has been invited and has accepted to ... January 7-10, 2008 at,the Westin St. Francis Hotel in ... Chief Executive Officer will provide an,overview of the Company ... About JPMorgan 26th Annual Healthcare Conference, JPMorgan,s premier ...
... safety and efficacy in treatment of multi-drug ... resistant infections ... or the "Company"), a biopharmaceutical company focused on the,development of novel ... the first patients in two,separate Phase 2 clinical trials. The studies ...
Cached Biology Technology:QuatRx Pharmaceuticals to Present at 26th Annual JP Morgan Healthcare Conference 2Rib-X Pharmaceuticals Initiates Two Phase 2 Studies for Novel Antibiotic Compound RX-1741 2Rib-X Pharmaceuticals Initiates Two Phase 2 Studies for Novel Antibiotic Compound RX-1741 3
... The IEC Centra CL3 series ... in typical research and clinical applications ... broad accessory package includes quick connect ... An advanced yet intuitive interface allows ...
PP2B-Abeta (C-20)...
DNA Polymerase I Large (Klenow) Fragment is a DNA polymerase that lacks the 5'Cut Site3' exodeoxyribonuclease activity of intact DNA Polymerase I but it contains the 3'Cut Site5' exodeoxyribonuclease...
... H2OBIT™ is a fully licensed, high throughput, water ... of up to 24 microplates. This equates to ... A robotic arm rapidly transfers a ... in temperature ramping times that are considerably faster ...
Biology Products: