Navigation Links
UF researchers develop blood test for devastating disease of boas and pythons
Date:1/29/2014

GAINESVILLE, Fla. University of Florida researchers have developed a simple immune-based screening test to identify the presence of a debilitating and usually fatal disease that strikes boas and pythons in captivity as well as those sold to the pet trade worldwide.

Known as inclusion body disease, or IBD, the highly infectious disease most commonly affects boa constrictors but pythons and other snake species in the boid family are also occasionally infected with the virus that causes the disease. IBD was first seen in snakes in the late 1970s, said Elliott Jacobson, D.V.M., Ph.D., a professor emeritus of zoological medicine at the UF College of Veterinary Medicine and co-author of a study that appeared in December in PLOS ONE.

"We don't know the prevalence, but we see more of IBD in the United States because there are some 2 million boas being kept as pets in this country," Jacobson said. "This simple blood test will help determine whether or not an animal has this disease and potentially will help clean up colonies of snakes that will ultimately be disease-free."

Although snakes infected with IBD may display neurological signs, such as head-tilting, chronic regurgitation or disequilibrium, there is also a population of snakes that are subclinical, meaning they are infected but otherwise appear healthy.

"That's a big problem, because healthy-seeming animals that are affected with IBD are being sold and sent around the world," he said. "However, they may develop the disease sometime later and may be the source of infection for other snakes."

On Jacobson's research team at the UF veterinary college were his former graduate student, Li-Wen Chang, B.V.M., Ph.D., the principal investigator in the study, and Jorge Hernandez, D.V.M., Ph.D., a veterinary epidemiologist.

To develop the test, the researchers studied a monoclonal antibody produced in response to a unique protein that accumulates in cells of snakes having IBD. They then sequenced the protein in an effort to further understand the nature and cause of the disease. Although the cause of IBD is unclear, the UF team found genetic links of this unique protein are associated with a family of viruses that primarily infect rodents but may infect humans. However, there is no evidence to indicate that the virus that causes IBD can infect people.

When Chang joined the study in 2008, she realized the limited availability of snake databases and potential causative agents of the disease presented additional challenges.

"It took us almost a year to finally produce this antibody, and three more years to validate its performance for immuno-based diagnostic tests," Chang said.

University of California-San Francisco researchers identified the Golden Gate virus in 2012 and scientists now consider it to be a potential cause of IBD.

UF's findings supplement that theory, although more studies of disease transmission need to be conducted to confirm the role of Golden Gate virus in the development of IBD, Jacobson said.

The research was performed at the UF's Interdisciplinary Center for Biotechnology Research through the university's veterinary diagnostic laboratories, where the new test is now offered. It will supplement existing molecular and histological tests, which are more widely available but also more expensive, Jacobson added. In addition, the test's ease of use and simplicity will offer veterinary practitioners a good first-line diagnostic tool to screen for IBD in snake species that show signs of the disease, or even before these signs occur.

"We know now that this disease exists in multiple collections and populations," Jacobson said. "It is important to determine why some snakes are not showing clinical signs of the disease. Could there be another agent operating synergistically? Perhaps one virus needs to be present but another virus needs to be present also, or perhaps the subclinical cases only have one of those agents, not both."

Only strict quarantine of new arrivals to snake populations and the culling of infected snakes, as well as mite control, can mitigate the spread of the disease, according to a 2013 fact sheet prepared by the American Association of Zoo Veterinarians' infectious disease committee.

"It's a situation of management," Jacobson said. "You'll never completely eradicate this disease."


'/>"/>

Contact: Sarah Carey
careysk@ufl.edu
352-294-4242
University of Florida
Source:Eurekalert  

Related biology news :

1. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
2. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
3. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
4. UNH researchers find African farmers need better climate change data to improve farming practices
5. Ottawa researchers to lead world-first clinical trial of stem cell therapy for septic shock
6. Researchers uncover molecular pathway through which common yeast becomes fungal pathogen
7. Researchers print live cells with a standard inkjet printer
8. Columbia Engineering and Penn researchers increase speed of single-molecule measurements
9. Researchers reveal how a single gene mutation leads to uncontrolled obesity
10. Researchers discover novel therapy for Crohns disease
11. New paper by Notre Dame researchers describes method for cleaning up nuclear waste
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
UF researchers develop blood test for devastating disease of boas and pythons
(Date:3/18/2016)... LONDON , March 18, 2016 ... Established Suppliers of Biometrics, ICT, Manned & Unmanned Vehicles, Physical ... & security companies in the border security market and ... and Europe has led ... your companies improved success. --> defence & ...
(Date:3/14/2016)... 2016 http://www.apimages.com ) - ... - Renvoi : image disponible via AP Images ... --> DERMALOG, le leader de ... lecteurs d,empreintes digitales pour l,enregistrement des réfugiés en ... pour produire des cartes d,identité aux réfugiés. DERMALOG ...
(Date:3/9/2016)... March 9, 2016 This BCC Research report ... of the RNA Sequencing (RNA Seq) market for the ... instruments, tools and reagents, data analysis, and services. ... of the RNA-Sequencing market such as RNA-Sequencing tools and ... main factors affecting each segment and forecast their market ...
Breaking Biology News(10 mins):
(Date:5/25/2016)... ... 25, 2016 , ... Scientists at the University of Athens say they have ... be hampering the research that could lead to one good one. Surviving Mesothelioma has ... now. , The team evaluated 98 mesothelioma patients who got a ...
(Date:5/24/2016)... 2016   MedyMatch Technology Ltd ., the data analytics ... decision support tools in the emergency room, announced today that ... Advanced Technology Industries (IATI) BioMed Conference. The ... 15th National Life Sciences and Technology Week, and is being ... in Tel Aviv, Israel . Gene ...
(Date:5/23/2016)... -- Zimmer Biomet Holdings, Inc. (NYSE and SIX: ZBH), a ... of Directors has approved the payment of a quarterly cash ... The cash dividend of $0.24 per share will ... of record as of the close of business on June ... of the Board of Directors and may be adjusted as ...
(Date:5/22/2016)... ... ... Doctors in Rome say micronutrients found in certain foods have the potential to ... posted an article on the new research. Click here to read it now. ... Medicine evaluated more than 150 studies on polyphenols in cancer for their report recently ...
Breaking Biology Technology: