Navigation Links
UCLA researchers engineer E. coli to produce record-setting amounts of alternative fuel
Date:3/17/2011

Researchers at UCLA's Henry Samueli School of Engineering and Applied Science have developed a way to produce normal butanol often proposed as a "greener" fuel alternative to diesel and gasoline from bacteria at rates significantly higher than those achieved using current production methods.

The findings, reported online in the journal Applied and Environmental Microbiology, mark an important advance in the production of normal butanol, or n-butanol, a four-carbon chain alcohol that has been shown to work well with existing energy infrastructure, including in vehicles designed for gasoline, without modifications that would be required with other biofuels.

The UCLA team, led by James C. Liao, UCLA's Chancellor's Professor of Chemical and Biomolecular Engineering, demonstrated success in producing 15 to 30 grams of n-butanol per liter of culture medium using genetically engineered Escherichia coli a record-setting increase over the typical one to four grams produced per liter in the past.

For the study, Liao and his team initially constructed an n-butanol biochemical pathway in E. coli, a microbe that doesn't naturally produce n-butanol, but found that production levels were limited. However, after adding metabolic driving forces to the pathway, the researchers witnessed a tenfold increase in the production of n-butanol. The metabolic driving forces pushed the carbon flux to n-butanol.

"Like human beings, microbes need an incentive to work," said Liao, the study's senior author.

"We created driving forces by genetically engineering the metabolism," said Claire R. Shen, a UCLA Engineering graduate student and lead author of the study.

While certain microbes, including species of the bacteria Clostridium, naturally produce n-butanol, Liao's team used E. coli because it is easier to manipulate and has been used industrially in producing various chemicals.

"By using E. coli, we can make it produce only the compound with no other byproducts," Liao said. "With native producing organisms like Clostridium, which naturally produces n-butanol, there are other byproducts that would add cost to the separation process."

The next step in the research, the researchers say, will be to transfer the study to industry for the development of a more robust industrial process.


'/>"/>

Contact: Wileen Wong Kromhout
wwkromhout@support.ucla.edu
310-206-0540
University of California - Los Angeles
Source:Eurekalert

Related biology news :

1. Hopkins researchers use light to move molecules
2. Zoo researchers provide African sanctuaries road map
3. Brandeis researchers use lasers, custom microscope to show gene splicing process in real time
4. Synthetic biology: TUM researchers develop novel kind of fluorescent protein
5. Researchers find drug that stops progression of Parkinsons disease in mice
6. Researchers discover new wintering grounds for humpback whales using sound
7. Berkeley Lab researchers illuminate laminins role in cancer formation
8. Researchers discover new shapes of microcompartments
9. Researchers find possible new treatment strategies for pancreatic cancer
10. Scripps oceanography researchers discover arctic blooms occurring earlier
11. Nature study: Jefferson researchers unravel proteins elusive role in embryo and disease development
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/22/2016)... LOS ANGELES , June 22, 2016 ... of identity management and verification solutions, has ... cutting edge software solutions for Visitor Management, ... ® provides products that add functional ... The partnership provides corporations and venues with ...
(Date:6/21/2016)... June 21, 2016 NuData Security announced today ... role of principal product architect and that ... of customer development. Both will report directly to ... The moves reflect NuData,s strategic growth in its ... high customer demand and customer focus values. ...
(Date:6/16/2016)... The global Biometric ... USD 1.83 billion by 2024, according to a ... proliferation and increasing demand in commercial buildings, consumer ... the market growth.      (Logo: ... of advanced multimodal techniques for biometric authentication and ...
Breaking Biology News(10 mins):
(Date:12/2/2016)... , Dec 2, 2016 Research and ... "Nanobiotechnology Applications, Markets and Companies" to their offering. ... , , ... by the pharmaceutical and biotechnology industries is anticipated. Nanotechnology will ... formulations for optimal delivery to diagnostic applications in clinical trials. ...
(Date:12/2/2016)... ... December 01, 2016 , ... Aerocom Healthcare ... American hospitals, will present its chain-of-custody solution for tracking and securing medications at ... Nev., Dec. 4-8, 2016. , Aerocom has a proven solution for tracking medications ...
(Date:12/2/2016)... (PRWEB) , ... December 01, 2016 , ... DrugDev ... industry-wide collaboration, standardization and a beautiful technology experience. All three tenets were on display ... 100 clinical trial leaders from over 40 sponsor, CRO and site organizations to discuss ...
(Date:12/2/2016)... ... December 02, 2016 , ... Robots will storm the Prudential Center in Boston, ... The event, which is held on the United Nations International Day of Persons with ... into the workplace. Suitable Technologies is partnering with NTI to showcase how technology can ...
Breaking Biology Technology: