Navigation Links
UCLA researchers develop way to strengthen proteins with polymers
Date:5/21/2012

Proteins are widely used as drugs insulin for diabetics is the best known example and as reagents in research laboratories, but they react poorly to fluctuations in temperature and are known to degrade in storage.

Because of this instability, proteins must be shipped and stored at regulated temperatures, resulting in increased costs, and sometimes must be discarded because their "active" properties have been lost. Manufacturers of protein drugs will generally add substances known as excipients, like polyethylene glycol, to the proteins to prolong their activity.

In a new study published in the Journal of the American Society of Chemistry (DOI: 10.1021/ja2120234), investigators from the UCLA Department of Chemistry and Biochemistry and the California NanoSystems Institute at UCLA (CNSI) describe how they synthesized polymers to attach to proteins in order to stabilize them during shipping, storage and other activities. The study findings suggest that these polymers could be useful in stabilizing protein formulations.

The polymers consist of a polystyrene backbone and side chains of trehalose, a disaccharide found various plants and animals that can live for long periods with very little or no water. An example many people will recognize is Sea- Monkeys the 'novelty aquarium pet' introduced in 1962. SeaMonkeys can be purchased as kits that contain a white powder; when water is added, the powder becomes small shrimp whose long tails are said to resemble those of monkeys.

Trehalose is known to stabilize proteins when water is removed, and as a result, it is an additive in several protein drug formulations approved by the Food and Drug Administration (FDA) to treat cancer and other conditions.

"Our polymers were synthesized by a controlled radical polymerization technique called reversible addition-fragmentation chain transfer (RAFT) polymerization in order to have end groups that can attach to proteins to form what is called a protein-polymer conjugate," said Heather Maynard, a UCLA associate professor of chemistry and biochemistry and a member of the CNSI. "We found that the polymers significantly stabilized the protein we used lysozyme better to lyophilization (freeze-drying, in which water is removed from the protein) and to heat than did the protein with no additives."

The research team found that attaching the polymer covalently to the protein that is, forming a protein-polymer conjugate stabilized the protein to lyophilization better than adding the non-conjugated polymer at the same concentration.

The team also found that the polymers stabilized lysozyme significantly better than the currently used excipients trehalose and polyethylene glycol, depending on the stress and conditions used.

The Maynard research group is currently exploring the use of their polymer as a stabilizer by attaching it or adding it to FDAapproved protein therapeutics. In addition, they are investigating the mechanism of how the polymer stabilizes proteins.

The research team included Rock J. Mancini and Juneyoung Lee, both graduate students of chemistry and biochemistry in the Maynard research group.


'/>"/>

Contact: Jennifer Marcus
jmarcus@cnsi.ucla.edu
310-267-4839
University of California - Los Angeles
Source:Eurekalert

Related biology news :

1. Tufts Medical Center researchers finds marker in premies saliva predicts readiness to feed by mouth
2. Researchers aim to assemble the tree of life for all 2 million named species
3. Dartmouth researchers are learning how exercise affects the brain
4. Nottingham researchers lead worlds largest study into pre-eclampsia
5. Begin early: Researchers say water with meals may encourage wiser choices
6. Researchers look to relatives for clues in quest to develop sources of bioenergy
7. WSU researchers say genes and vascular risk modify effects of aging on brain and cognition
8. KIT researchers succeed in realizing a new material class
9. Sloppy shipping of human retina leads IU researchers to discover new treatment path for eye disease
10. UCSB researchers find that less is more, for female cowbirds
11. Researchers discover first gene linked to missing spleen in newborns
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/20/2016)... -- VoiceIt is excited to announce its new marketing ... working together, VoiceIt and VoicePass will offer an ... slightly different approaches to voice biometrics, collaboration between ... Both companies ... "This marketing and technology partnership allows VoiceIt ...
(Date:5/3/2016)... , May 3, 2016  Neurotechnology, a ... the MegaMatcher Automated Biometric Identification System (ABIS) ... large-scale multi-biometric projects. MegaMatcher ABIS can process multiple ... using any combination of fingerprint, face or iris ... MegaMatcher SDK and MegaMatcher Accelerator , ...
(Date:4/26/2016)... -- Research and Markets has announced the ...  report to their offering.  , ,     (Logo: ... forecast the global multimodal biometrics market to grow ... 2016-2020.  Multimodal biometrics is being implemented ... healthcare, BFSI, transportation, automotive, and government for controlling ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... , June 24, 2016 Epic Sciences ... detects cancers susceptible to PARP inhibitors by targeting ... cells (CTCs). The new test has already been ... in multiple cancer types. Over 230 ... damage response pathways, including PARP, ATM, ATR, DNA-PK ...
(Date:6/23/2016)... , ... June 23, 2016 , ... ... release of its second eBook, “Clinical Trials Patient Recruitment and Retention Tips.” Partnering ... retention in this eBook by providing practical tips, tools, and strategies for clinical ...
(Date:6/23/2016)... June 23, 2016 /PRNewswire/ - FACIT has announced ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" ... commercialization of a portfolio of first-in-class WDR5 inhibitors ... such as WDR5 represent an exciting class of ... precision medicine for cancer patients. Substantial advances have ...
(Date:6/23/2016)... Lawrence, MA (PRWEB) , ... June 23, 2016 ... ... the Peel Plate® YM (Yeast and Mold) microbial test has received AOAC Research ... test platform of microbial tests introduced last year,” stated Bob Salter, Vice President ...
Breaking Biology Technology: