Navigation Links
UCLA engineers develop new metabolic pathway to more efficiently convert sugars into biofuels

UCLA chemical engineering researchers have created a new synthetic metabolic pathway for breaking down glucose that could lead to a 50 percent increase in the production of biofuels.

The new pathway is intended to replace the natural metabolic pathway known as glycolysis, a series of chemical reactions that nearly all organisms use to convert sugars into the molecular precursors that cells need. Glycolysis converts four of the six carbon atoms found in glucose into two-carbon molecules known acetyl-CoA, a precursor to biofuels like ethanol and butanol, as well as fatty acids, amino acids and pharmaceuticals. However, the two remaining glucose carbons are lost as carbon dioxide.

Glycolysis is currently used in biorefinies to convert sugars derived from plant biomass into biofuels, but the loss of two carbon atoms for every six that are input is seen as a major gap in the efficiency of the process. The UCLA research team's synthetic glycolytic pathway converts all six glucose carbon atoms into three molecules of acetyl-CoA without losing any as carbon dioxide.

The research is published online Sept. 29 in the peer-reviewed journal Nature.

The principal investigator on the research is James Liao, UCLA's Ralph M. Parsons Foundation Professor of Chemical Engineering and chair of the chemical and biomolecular engineering department. Igor Bogorad, a graduate student in Liao's laboratory, is the lead author.

"This pathway solved one of the most significant limitations in biofuel production and biorefining: losing one-third of carbon from carbohydrate raw materials," Liao said. "This limitation was previously thought to be insurmountable because of the way glycolysis evolved."

This synthetic pathway uses enzymes found in several distinct pathways in nature.

The team first tested and confirmed that the new pathway worked in vitro. Then, they genetically engineered E. coli bacteria to use the synthetic pathway and demonstrated complete carbon conservation. The resulting acetyl-CoA molecules can be used to produce a desired chemical with higher carbon efficiency. The researchers dubbed their new hybrid pathway non-oxidative glycolysis, or NOG.

"This is a fundamentally new cycle," Bogorad said. "We rerouted the most central metabolic pathway and found a way to increase the production of acetyl-CoA. Instead of losing carbon atoms to CO2, you can now conserve them and improve your yields and produce even more product."

The researchers also noted that this new synthetic pathway could be used with many kinds of sugars, which in each case have different numbers of carbon atoms per molecule, and no carbon would be wasted.

"For biorefining, a 50 percent improvement in yield would be a huge increase," Bogorad said. "NOG can be a nice platform with different sugars for a 100 percent conversion to acetyl-CoA. We envision that NOG will have wide-reaching applications and will open up many new possibilities because of the way we can conserve carbon."

The researchers also suggest this new pathway could be used in biofuel production using photosynthetic microbes.

The paper's other author is Tzu-Shyang Lin, who recently received a bachelor's degree from UCLA in chemical engineering.


Contact: Matthew Chin
University of California - Los Angeles

Related biology news :

1. Stanford engineers monitor heart health using paper-thin flexible skin
2. Natures phenomena might teach Virginia Tech engineers new tricks
3. Cornell engineers solve a biological mystery and boost artificial intelligence
4. Iowa State computer, electrical engineers working to help biologists cope with big data
5. Maintaining Earths sustainability: Scientists, engineers, educators take coordinated approach
6. Nanoengineers can print 3D microstructures in mere seconds
7. WHOI scientists/engineers partner with companies to market revolutionary new instruments
8. Medusa reimagined: Caltech-led team reverse engineers a jellyfish with the ability to swim
9. BYU engineers conceive disc replacement to treat chronic low back pain
10. Civil engineers find savings where the rubber meets the road
11. Engineers use droplet microfluidics to create glucose-sensing microbeads
Post Your Comments:
(Date:11/12/2015)... 11, 2015   Growing need for low-cost, ... has been paving the way for use of ... discrete analytes in clinical, agricultural, environmental, food and ... used in medical applications, however, their adoption is ... to continuous emphasis on improving product quality and ...
(Date:11/10/2015)... , Nov. 10, 2015 ... behavioral biometrics that helps to identify and verify ... Signature is considered as the secure and accurate ... identification of a particular individual because each individual,s ... accurate results especially when dynamic signature of an ...
(Date:11/4/2015)... 4, 2015 --> ... published by Transparency Market Research "Home Security Solutions Market - ... 2015 - 2022", the global home security solutions market is expected ... 2022. The market is estimated to expand at a ... to 2022. Rising security needs among customers at homes, ...
Breaking Biology News(10 mins):
(Date:11/24/2015)... , Nov. 24, 2015 /CNW/ - iCo Therapeutics ("iCo" ... reported financial results for the quarter ended September ... in Canadian dollars and presented under International Financial ... States ," said Andrew Rae , ... regarding iCo-008 are not only value enriching for ...
(Date:11/24/2015)... (PRWEB) , ... November 24, 2015 , ... ... event of the year and one of the premier annual events for pharmaceutical ... ran from 8–11 November 2015, where ISPE hosted the largest number of attendees ...
(Date:11/24/2015)... Nov. 24, 2015  Clintrax Global, Inc., a worldwide provider of ... , today announced that the company has set a new quarterly ... quarter on quarter growth posted for Q3 of 2014 to Q3 ... Mexico , with the establishment of an ... --> United Kingdom and ...
(Date:11/24/2015)... ... ... This fall, global software solutions leader SAP and AdVenture Capital brought together ... their BIG ideas to improve health and wellness in their schools. , Now, the ... title of SAP's Teen Innovator, an all-expenses paid trip to Super Bowl 50, and ...
Breaking Biology Technology: