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UCI scientists decode genomes of sexually precocious fruit flies
Date:9/16/2010

Irvine, Calif., Sept. 16, 2010 UC Irvine researchers have deciphered how lowly fruit flies bred to rapidly develop and reproduce actually evolve over time. The findings, reported in the Sept. 15 online issue of Nature, contradict the long-held belief that sexual beings evolve the same way simpler organisms do and could fundamentally alter the direction of genetic research for new pharmaceuticals and other products.

"This is actually decoding the key DNA in the evolution of aging, development and fertility," said ecology & evolutionary biology professor Michael Rose, whose laboratory began breeding the "super flies" used in the current study in 1991 or 600 generations ago. He joked that they "live fast and die young."

Lead author and doctoral student Molly Burke compared the super flies to a control group on a genome-wide basis, the first time such a study of a sexually reproducing species has been done. The work married DNA "soup" gathered from the adapted flies with cheap, efficient technology that uses cutting-edge informatics tools to analyze the DNA of entire organisms. Burke found evidence of evolution in more than 500 genes that could be linked to a variety of traits, including size, sexual maturation and life span, indicating a gradual, widespread network of selective adaptation.

"It's really exciting," she said. "This is a new way of identifying genes that are important for traits we're interested in as opposed to the old hunting and pecking, looking at one gene at a time."

For decades, most researchers have assumed that sexual species evolve the same way single-cell bacteria do: A genetic mutation sweeps through a population and quickly becomes "fixated" on a particular portion of DNA. But the UCI work shows that when sex is involved, it's far more complicated.

"This research really upends the dominant paradigm about how species evolve," said ecology & evolutionary biology professor Anthony Long, t
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Contact: Janet Wilson
janethw@uci.edu
949-824-3969
University of California -- Irvine
Source:Eurekalert

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Size & Applications: 100 ul (2 mM). Recommend to use at 1000X dilutions for cell culture (e.g. Add 1 ul to 1 ml of culture medium.) Storage Temp.: -20C Shelf Life: 6-12 month...
NM522 contains an F episome, which is required for production of ssDNA and for blue/white color selection. To maintain the F, NM522 should be grown on minimal (M-9) medium....
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