Navigation Links
UC San Diego and Genentech scientists develop potentially disruptive antibody sequencing technology
Date:12/19/2008

it2). Bandeira's co-authors on the Nature Biotechnology paper include UC San Diego computer science and engineering professor Pavel Pevzner, director of the Calit2-based Center for Algorithmic and Systems Biology (CASB); and three researchers from the Protein Chemistry Department of San Francisco-based Genentech: Victoria Pham, David Arnott, and Jennie R. Lill.

Shotgun protein sequencing will be particularly useful when complementary DNA (cDNA) or the original cell line is not available, or if there is a need to verify the integrity and effectiveness of an antibody after the cell has undergone changes subsequent to the original sequencing.

"Antibodies are indispensable in biomedical research and they are widely used as diagnostic and therapeutic agents," said Genentech's Jennie Lill. "DNA sequencing is routinely used in the initial characterization of monoclonal antibodies, but subsequent mutations and other changes mean that further protein level analysis is needed. So it is critical to sequence the antibodies for a variety of reasons, from monitoring the integrity of the molecule, to troubleshooting performance in pre-clinical assays."

Until now, the only viable option for sequencing an antibody has been a process known as Edman degradation, named for Swedish chemist Pehr Edman. (The technique was used in the sequencing of insulin, for which the Nobel Prize in Chemistry was awarded in 1958.) While Edman degradation remains a low-throughput and time-consuming approach, no fast substitute for this technique was found in the last half-century.

Bandeira and his colleagues proposed to substitute this ancient technique with protein sequencing based on mass spectrometry.

While mass spectrometry routinely is used to sequence short fragments of proteins (called peptides), no techniques for sequencing entire proteins were available until recently. The key bottleneck has been computing rather than experiment, since the challenge o
'/>"/>

Contact: Doug Ramsey
dramsey@ucsd.edu
858-822-5825
University of California - San Diego
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. UC San Diego physicists tackle knotty puzzle
2. UC-San Diego Engineering Honor Society wins most outstanding chapter award
3. Experimental Biology 2008 meets in San Diego April 5-9
4. UC San Diego begins trading greenhouse gas credits on Chicago Climate Exchange
5. San Diego Harbor Police Deploy BIO-key(R) Automated Vehicle Location System
6. Biologists at UC San Diego identify key protein in cells self-eating function
7. UC San Diego researchers eliminate drug discovery bottleneck
8. Glowing films developed by UC San Diego chemists reveal traces of explosives
9. UC San Diego researchers identify potential new drug candidates to combat bird flu
10. UC San Diego launches Institute of Engineering in Medicine to accelerate health care tech
11. UC San Diego researchers could help US military thwart explosive threats
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
UC San Diego and Genentech scientists develop potentially disruptive antibody sequencing technology
(Date:7/11/2014)... new report from the Wildlife Conservation Society shows ... help economically valuable species such as lobster, conch, ... help re-colonize nearby reef areas. , The reporttitled ... systematic review of research literature from no-take areas ... Dr. Craig Dahlgren, a recognized expert in marine ...
(Date:7/11/2014)... were many fires burning in eastern New South Wales, ... 11. , At 03:35 UTC on July 11 (12:35 ... Aqua passed over eastern New South Wales (NSW), the ... visible image of the region and spotted smoke (light ... MODIS,s thermal bands, are outlined in red. , The ...
(Date:7/11/2014)... and Mineral Research awarded Michael F. Holick, PhD, MD, ... 2014 Louis V. Avioli Award. Holick, a professor of ... for revolutionizing the understanding of vitamin D and its ... a member of the American Society for Bone and ... mineral basic research. It is named for ASBMR,s first ...
Breaking Biology News(10 mins):Belize's lobster, conch, and fish populations rebuild in no-take zones 2BUSM's Michael F. Holick receives American Society for Bone and Mineral Research award 2
... from the Buck Institute for Age Research offers a ... neurodegenerative disorder which afflicts 24 million people worldwide. ... a protein implicated in AD, scientists in California and ... a new therapeutic target for the disease. The finding ...
... fish do it. But Prof. Yossi Loya from Tel Aviv ... to discover that Japanese sea corals engage in "sex switching" ... survival of fragile sea corals ― essential to all life ... In times of stress like extreme hot spells, the ...
... group of scientists is renewing calls for policymakers to ... eutrophication or nutrient pollution problems in fresh ... Science , the researchers argue that dual-nutrient reduction ... complex interactions between nitrogen and phosphorus in fresh and ...
Cached Biology News:A revolutionary new model for Alzheimer's disease 2Changing sexes on the sea floor 2
(Date:7/10/2014)... Ouagadougou, Burkina Faso (PRWEB) July 10, 2014 ... Burkina Faso, today have access to an additional ... to increase access to contraception at all levels ... combining a lower-dose formulation of a widely used ... injection system. , Burkina Faso is the first ...
(Date:7/10/2014)... Understanding the need for better quality and ... gap that currently exists in this area, ChanTest has ... of validated ion channel and GPCR cell lines. ... ion channels control many critical physiological functions throughout the ... potential to treat a variety of human diseases. ...
(Date:7/10/2014)... (PRWEB) July 10, 2014 Robert Harman, DVM, ... Medicine company, is proud to announce the relaunch of his ... with a new series called “ What are Stem Cells ... an honest and straightforward foundation in the basics of stem ... decision on the right type of treatment when considering regenerative ...
(Date:7/10/2014)... CA (PRWEB) July 10, 2014 Senior ... institutions and professional associations, addressed the challenges of “Reducing ... Medical Device Industries” through strategic sourcing. Describing the partnership ... with the Supply Chain Management Institute ( SCMI) ... event on June 24 at the Kroc Institute of ...
Breaking Biology Technology:Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 2Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 3Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 4Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 5Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 6Leader in Ion Channel Research Announces Newly Validated and Re-Engineered Cells 2Leader in Ion Channel Research Announces Newly Validated and Re-Engineered Cells 3Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 2Robert Harman, DVM Talks About What Stem Cells are in His Latest Blog Series for Vet-Stem, Inc. 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 2Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 4
... Nev., Sept. 30, 2011 Lifeline Biotechnologies, Inc. (Pink Sheets: ... efforts to reestablish DTC services. Jim Holmes, Lifeline,s ... services to the Company we have not been successful.  We ... The value of Lifeline is not reflected in ...
... , SALT LAKE CITY, Sept. 29, 2011 University of ... catalysts, which are substances that speed chemical reactions and are ... the new method, the chemists also made a discovery that ... discovery: the sizes and electronic properties of catalysts interact to ...
... BioSpace , the leading life sciences employment and ... marking the 14th edition. (Logo: http://photos.prnewswire.com/prnh/20040907/CLTU006LOGO) ... Each company,s brand is added to an electronic and ... as well as at industry events and career fairs. ...
Cached Biology Technology:Lifeline Biotechnologies and Depository Trust Company 2Building better catalysts 2Building better catalysts 3BioSpace Spotlights Southern California's Life Science Community 2
... Andrew J. Davison and ... • Contents include: information ... viruses, paramyxoviruses, influenza and ... covers analysis of viral ...
... U. Desselberger (1995) • This book ... together classical and molecular techniques currently ... infections and analysis of virus-specific antibodies ... are presented covering immunoassays, blotting and ...
...
... provide rapid and efficient removal of short ... salts from PCR products (Figure 1). The ... Binding Buffer options for fragments >100 bp ... in either single column or 96-well plate ...
Biology Products: