Two University of British Columbia researchers are part of an international team recommending standards for the DNA barcoding of land plants, a step they hope will lead to a universal system for identifying over 400,000 species, and ultimately boost conservation efforts.
Barcodes based on portions of DNA the taxonomical equivalent to UPC barcodes on products have already emerged as a viable solution for uniquely identifying species in many animal groups. However, because DNA varies less between plant species, determining which portions of plant DNA to use as a unique identifier has been a thorny issue.
The research team, which included scientists from more than 20 institutions around the world, selected two genomic regions genes referred to as rbcL and matK as the best candidates from which to generate barcode data.
Results of the four-year study are published this week in the Proceedings of the National Academy of Sciences.
"It's a pragmatic first step in solving a complex issue," says UBC botanist and Associate Professor Sean Graham, who conducted research on the project and helped author the study. "We've selected areas of DNA that are available in the vast majority of plants, could easily and accurately be sequenced, and when combined, provide a near-unique signature for barcoding."
Limiting the barcode to information generated from two DNA sites should help cut costs associated with sequencing and retrieving the correct information.
The researchers used 400 land plant samples to test the two-site solution. In 72% of cases they were immediately able to determine the correct species of plant, and in the rest of the cases were able to place the plant in a group of congeneric species.
"There's no doubt this will be refined in the future, but there is a need for a core barcoding standard now," says Graham, with the UBC Botanical Garden and Centre for Plant Research, and the Department o
|Contact: Brian Lin|
University of British Columbia